Aim To study the protective effect of fructose-1,6-diphosphate(FDP) on adriamycin(ADR)-induced myocardial damage. Methods 30 SD rats were divided into three groups randomly: control group,ADR group and ADR+FDP group.The changes of heart rate and dry and wet ratio of pulmonary and liver were observed,and the level of creative kinase isoenzyme MB(CK-MB) were measured.The active level of CuZn SOD was examined and its protein level was measured by immunohistochemistry,its gene level expression was measured by half-quantitatire polymerase chain reaction. Result Compared with ADR group,the heart rate change of ADR+FDP group was decreased(p<0.05),the dry and wet ratio was increased(p<0.05),and CK-MB was decreased(p<0.01) obviously.The active level of Cn-Zn SOD was up(p<0.05),the gene and protein expression of Cu-Zn SOD was down(p<0.05). Conclusion FDP has a protective effect on adriamycin-induced myocardial damage.Its mechanism is possibly related with inhibiting oxidation.