Aim To investigate the relationship between cytokines expression and inflammatory response after myocardial infarction in mice.Methods Develope mice model of myocardial infarction by coronary artery ligation.Levels of serum tumor necrosis factor-α,interleukin-6 and interleukin-10 were assayed by ELISA,and pathology of myocardium was detected by histochemistry.Results Expressions of tumor necrosis factor-α(82.4±27.6 ng/L) and interleukin-10(47.7±22.3 ng/L) were found to be significantly higher in 1 h after myocardial infarction than that of sham operated groups(40.4±9.7 ng/L and 23.4±4.3 ng/L),and they decreased gradually after that;Expressions of interleukin-6(202.7±89.5 ng/L,578.9±198.0 ng/L,696.8±104.7 ng/L,325.1±131.7 ng/L,218.5±16.0 ng/L,and 195.8±93.1 ng/L) were also found to be significantly higher in 1 h,2 h,4 h,1 d,2 d,and 5 d after myocardial infarction than that of sham operated groups(54.0±3.0 ng/L).Leucocytes recruited in the infarcted myocardium after myocardial infarction.Inflammatory cytokines decreased during 2 weeks and 4 weeks after myocardial infarction,and Leucocytes in the infarcted myocardium decreased gradually.Myocardium fibrosis was found.Conclusion The changes of tumor necrosis factor-α,interleukin-6 and interleukin-10 take part in the inflammatory response after myocardial infarction,and play an important role in the development of myocardial infarction diseases.