Aim To investigate the effect of selective retrograde coronary venous bypass (SRCVB) on nitric oxide (NO) and endothelin (ET) of myocardial ischemia model in dogs. Methods 16 dogs were divided into ischemia group (n=8) and SRCVB group (n=8). The levels of plasma NO and ET were measured in each group. In SRCVB group, 10 mL ink were utilized to perfuse the heart through SVG graft in vivo. Results The differences of NO and ET were not statistically significant before ligation of PDA in both groups (p>0.05), but changed significantly in SRCVB group after the ligation (p<0.01). The ink perfused retrogradely from graft distributed the myocardium tissue uniformly. Conclusions SRCVB could guarantee effective perfusion of myocardium and ameliorate the abnormal concentration of NO and ET in ischemic myocardium, decrease injury to endothelial cell and protect the heart.