Aim To evaluate the efficiency of endovascular stent-based gene delivery system using antibody tethered plasmid DNA. Methods Endovascular stents were formulated with a collagen coating.Anti-DNA antibodies were covalently bound to the collagen surface by a cross linking reagent and then pEGFP-C1(enhanced green fluorescent protein) was immunobound to endovascular stent.Gene transduction efficiency was evaluated in cell culture.Furthermore,inducible nitric oxide synthase(iNOS) was chosen as therapeutic gene and bound to endovascular stent.Stents with antibody-tethered iNOS were implanted in pig coronary artery to evaluate the transduction efficiency and the effect of restenosis prevention. Results Gene delivery from stents carrying antibody-tethered pEGFP-C1 demonstrated efficient and site-specific pEGFP-C1 transduction in cell culture.GFP-positive cells were only observed in the site that directly contact with the collagen matrices and the transduction efficiecny was 21.8% vs less than 5% in control group.In pig coronary artery stent deployment studies,reverse transcription polyerase chain reaction(RT-PCR) analyses showed that iNOS was only observed in the blood vessel that contact with the stent,iNOS was undetectable in distal tissues such as lung,liver and spleen,etc. Conclusion Gene delivery system based on antibody immobilized coronary stents provided localized and highly efficient gene delivery for intravascular site-specific gene therapy.