AimTo investigate the change of plasma tissue factor (TF) level in the ischemic smoker and the healthy smoker, and discuss the influence of cigarette smoking on cerebral thrombosis.Methods Plasma TF levels of 45 smoking brain ischemic patients, 43 non-smoking brain ischemic patients, 42 healthy smokers and 45 healthy non-smokers were detected with two-layer antibody sandwich enzyme-linked immunosorbent assay (LWISA). ResultsThe plasma TF level of smoking brain ischemic group was obviously higher than that of non-smoking brain ischemic group (P<0.01) and healthy non-smoking group (P<0.01); TF level of healthy smoking group was obviously higher than that of non-smoking healthy group (P<0.01), and heavy smoking group was obviously higher than middle smoking group (P<0.05), little smoking group (P<0.01)and healthy group (P<0.01).The smoking index was positively correlated with the plasma TF value（r=0.43，P<0.01）.ConclusionThe improvement of plasma TF level induced by smoking may be a pathway through which smoking induce brain atherosclerosis and cerebral thrombosis.