Aim To compare the activities and mRNA expression levels of Na+,K+-ATPase and Ca²⁺+-ATPase in cultured human umbilical artery smooth muscle cells (hUAMSC) isolated from neonates with hypertensive family history (FH+)or without hypertensive family history(FH-),and to compare the concentration of angiotensin Ⅱ (Ang Ⅱ) and endothelin (ET) in hUASMCs supernatant and then explore the relationships between the ion pumps and autocrine Ang Ⅱ,ET in human umbilical artery smooth muscle cells from FH+. Methods The ion pumps activities in cultured hUASMC were detected by spectrophotography. The mRNA expression of Na+,K+-ATPase α1-subunit and plasma membrane Ca²⁺+-ATPase isoform 1 (PMCA1) in both FH+ and FH-hUASMC was measured by RT-PCR. Radioimmunoassay was used to determine the content of Ang Ⅱ and ET in supernatant of cultured hUASMC. Results The activities of Na+,K+-ATPase and Ca²⁺+-ATPase from FH+ hUASMC were higher than those of FH-group (P><0.05). But the mRNA expression of Na+,K+-ATPase α1-subunit and PMCA1 had no difference between FH+ and FH-. There was no significant difference in Ang Ⅱ,ET concentration in the supernatant of the two-group hUASMC. Conclusion The activities of Na+,K+-ATPase and Ca²⁺+-ATPase are increased in FH+ hUASMC,which may not be related to the mRNA expression of α1-subunit,PMCA1 and the autocrine of Ang Ⅱ and ET.