Aim To study the effects of endothelin-1(ET-1) and BQ-123 on activity and mRNA expression of adenosine triphosphatase(ATPase) in Aortic Smooth muscle cells(ASMC) from spontaneously hypertensive rats(SHR)and Wistar-Kyoto(WKY) rat. Methods The ASMC were isolated from SHR and WKY rats.ATPase activity in cultured ASMCs were determined by spectrophotography and mRNA level of Na+,K+-ATPase α1-subunit,PMCA1 were measured by semiquantitative reverse transcription polymerase chain reaction(RT-PCR). Results ET-1 significantly attenuated activity of two kinds of ATPase(P><0.01)and PMCA1 mRNA expression(P><0.01) in ASMC from SHR.BQ-123 increased two kinds of ATPase activity(P><0.01) and upregulated expression of PMCA1 mRNA(P><0.01),which treated by ET-1.The level of Na+,K+-ATPase α1-subunit mRNA expression had no alteration after intervened by ET-1(P>0.05). Conclusion ET-1 suppressed Na+,K+-ATPase,Ca²⁺+-ATPase activity,which may be mediated by ETA receptor.The influence of ET-1 Ca²⁺+-ATPase activity may play in the transcriptional level.BQ-123 reversed the effect of ET-1 on the activities of SHR ASMCs Na+,K+-ATPase and Ca²⁺+-ATPase by blocking the ETA receptor.