Aim To assess the association between the length of(GT)n repeats in the heme oxygenase-1(HO-1)gene promoter and the susceptibility for coronary heart disease(CHD)of Chaoshan population.Methods 300 patients with CHD and 182 control subjects with sex and age matching were collected.Amplification and labeling of PCR products for capillary electrophoresis were performed to genotype.(GT)n repeat polymorphism was classified into short(n≤25 for S allele)and long(n>25 for L allele)alleles.Results Allelic and genotypic frequency did not differ significantly between CHD patients and control subjects.However,there were interaction between genotype and smoking(OR was 1.790,95%CI was 1.110-2.886),hypertension(OR was 1.552,95%CI was 1.045-2.304)and diabetes(OR was 1.727,95%CI was 1.018-2.928).Specially,the SL+LL genotype had the highest risk(OR was 2.517,95%CI was 1.206-5.253).Conclusion Our findings indicated that longer(GT)n repeat allele in HO-1 promoter is a susceptibility marker of CHD with risk factors in Chaoshan population.