Aim To study the effect of basic fibroblast growth factor(bFGF) and platelet-derived growth factor-BB(PDGF-BB) on the proliferation and migration of endothelial progenitor cells(EPC)-derived endothelial-like cells and smooth muscle-like cells.Methods EPC isolated and cultured for 5 days were stained by immunofluorescence and observed with laser confocal microscopy,the cells of which both AC133 and vWF are positive are the differentiating EPC.After 5 days of EPC culture,the medium was replaced by either control medium(DMEM,supplemented with 20% fetal bovine serum) or control medium containing rat bFGF(30 μg/L) or PDGF-BB(40 μg/L).The cultures were incubated up to 14th day.Then,endothelial cell marker(CD31 and vWF) of bFGF-induced cells(FIC) and non-induced cells(NIC) or smooth muscle cell marker(α-SMA and calponin) of PDGF-BB-induced cells(PIC) and non-induced cells(NIC) were measured by confocal microscopy.The proliferation and migration of the endothelial-like cells and smooth muscle-like cells-derived EPC were detected by MTT assays and Transwell.Results Compared with control group,EPCs induced by bFGF(named as endothelial-like cells) or EPC induced by PDGF-BB(named as smooth muscle-like cells) showed respectively more significant endothelial mark(CD31,vWF) or smooth muscle mark(α-SMA,calponin);In the period of 0～48 h,bFGF and PDGF-BB respectively promoted endothelial-like cells and smooth muscle-like cells proliferation at the corresponding concentration and there were time-dependent and dose-dependent manner.Conclusion bFGF and PDGF-BB may promote EPC to differentiate into endothelial-like cells and smooth muscle-like cells,and these endothelial-like cells and smooth muscle-like cells have the obvious ability of proliferation and migration.