AimTo observe the regulatory effect of RhoA/Rho kinase in expression of tissure factor(TF) protein influenced by fluvastatin, so as to explore the noval target point of fluvastatin in anti-artherosclerosis and anti-thrombosis.MethodsThe same human umbilical vein endothelial cells (HUVEC) was cultured and passage cultured, and was then divided into control group, TNF-α group, Flu group, and TNF-α+Flu group, TNF-α+AngⅡ group, AngⅡ group and TNF-α+Y27632 group.RT-PCR method was used to examine the expression of TF mRNA.Western blot method was used to examine the TF protein expression and RhoA activity. TF protein expression of HUVECs in control group, TNF-α+AngⅡ group, AngⅡ group, TNF-α group and TNF-α+Y27632 group was examined using Western blot method. ResultsIn the meanwhile of inducing the HUVECS TF expression, TNF-α also activated RhoA.Fluvastatin inhibited the activation of RhoA.AngⅡ could enhance the TF expression in HUVECS which induced by TNF-α, while Y27632 could inhibit the TF expression in HUVECS which induced by TNF-α.ConclusionsThe inhibitive effect of fluvastatin on the TF expression might be achieved through the RhoA/ Rho signal regulatory pathway at small G protein level.