AimTo investigate the possible signaling pathway in angiopoietin-1 (Ang-1) regulated inflammatory factors of endothelial progenitor cells.MethodsWe transformated Ang-1 into tumor necrosis factor-α (TNF-α) induced endothelial progenitor cell (EPC), used the specific inhibitor pyrrolidine dithio carbamate (PDTC) to inhibit nuclear factor-kappa B (NF-κB).Western blot was used to detect the protein expression of Ang-1 and NF-κB, real-time polymerase chain reaction (real-time PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the mRNA and protein expression of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) in EPC.ResultsAng-1 protein expressed while no significant NF-κB protein expressed in EPC.Real-time PCR and ELISA show that compared to the TNF-α group, the expressions of ICAM-1 and VCAM-1 in Ang-1 group, PDTC group and Ang-1＋PDTC group significantly decreased (p<0.05), but there were no significant differences among the three groups (p＞0.05).ConclusionsAng-1 maybe affects adhesion molecules expression in TNF-α induced EPC through NF-κB signaling pathway.