AimTo investigate the effect of tail interacting protein of 47 kDa (TIP47) on lipid-droplets (LDs) formation and triglyceride accumulation in Macrophages.MethodsWestern blot was performed to detect the effects of TIP47 siRNA on the expression of TIP47 and adipose differentiation-related protein (ADRP).Oil-red O staining and lipid analysis were employed to measure the formation of LDs and accumulation of triglycerides in Macrophages.Results TIP47 siRNA clearly inhibited TIP47 protein expression, but did not affect the expression of ADRP protein.Furthermore, TIP47 siRNA suppressed ox-LDL induced LDs formation and triglycerides accumulation in macrophages.Conclusions The results indicates that TIP47 should be preventive or therapeutic target for diseases caused by excessive lipid accumulation through suppression of foam cells formation, such as atherosclerosis.