AimTo study the expression of insulin resistance-associated gene and to elucidate the molecular mechanisms in skeletal muscle tissue between fructose-fed rats and normal rats with cDNA microarray.MethodsThe rats were divided into two groups, one was normal and the other was fructose-rich chow.The mRNA was isolated and purified from skeletal muscle tissue of two groups.The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes.The mixed probes were hybridized to cDNA microarray .The microarray was scanned , analyzed and repeated for two times.ResultsAmong the total 4096 tested genes, 140 genes were differently expressed,62 up-regulated,78 down-regulated.ConclusionsGene expression profile is different from skeletal muscle tissue between fructose-fed rats and normal rats.The differential expression of genes may be related to the pathogenesis of insulin resistance.