Aim To study the influence of plasma advanced oxidation protein products （AOPPs） level on the contents of high density lipoprotein （HDL） subclasses. Methods Contents of AOPPs were detected by the chloramine-T method and the composition of HDL subclasses were measured with two-dimensional electrophoresis immunoblot in 346 subjects. According to the concentration of AOPPs,subjects were divided into three layers amount: low AOPPs （AOPPs≤60 μmol/L） group,middle AOPPs （60 μmol/L＜AOPPs＜90 μmol/L） group,high AOPPs （AOPPs≥90 μmol/L） group. Results Compared with the low AOPPs group,preβ₁-HDL and HDL_3a were significantly increased （P<0.001）,but lecithin-cholesterol acyltransferase （LCAT）,HDL_2a and HDL_2b were significantly decreased （P<0.001） in high AOPPs group. Cholesterol ester transfer protein （CETP） did not change in subjects. Correlation analysis showed AOPPs effect on changes in plasma HDL subclass. Conclusion With elevated plasma AOPPs concentration,the particle size of HDL shows a shift toward smaller size,AOPPs may hinder mature metabolism of HDL.