Aim To observe the effect of Cyclophilin A on the expression of nuclear factor-κB (NF-κB) protein in RAW264.7 cells induced by oxidized low density lipoprotein (ox-LDL). Methods Western blot was applied to detect the expression of Cyclophilin A and NF-κB protein in RAW264.7 cells induced by ox-LDL. Western blot was applied to detect the expression of Cyclophilin A and NF-κB protein in Cyclophilin A siRNA RAW264.7 cells induced by ox-LDL. Results The expression of Cyclophilin A and NF-κB protein in RAW264.7 cells was up-regulated by ox-LDL. Cyclophilin A siRNA could down-regulate the expression of NF-κB protein. Conclusion The change of the CypA protein expression could down-regulate the protein expression of NF-κB in RAW264.7 cells induced by ox-LDL.