AimTo study the effect and mechanism of rhynchophylla total alkaloids, sinapine cyanide sulfonate and its component compatibility on change of vascular endothelial cells induced by the tumor necrosis factor-α(TNF-α).MethodsRat vascular endothelial cells were cultured in vitro, and vascular endothelial cell injury model was established by TNF-α.Then changes of cell morphology were observed before and after treatment; intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1) and rat endothelin-1 (ET-1) were determined by Elisa method, mRNA of ICAM-1, VCAM-1 and ET-1 by RT-PCR method and the level change of endothelium-derived relaxing factor (NO) by nitrate reductase method.ResultsThe vascular endothelial cell injury model could be established in vitro successfully by TNF-α (5 μg/L).Compared with TNF-α group, treated groups could improve vascular endothelial cell morphology, lower the secretion of ICAM-1,VCAM-1 and ET-1.Compared with rhynchophylla total alkaloids group and sinapine cyanide sulfonate group, the component compatibility group was better on heightening the secretion of NO, down-regulating the expression of ICAM-1 mRNA(P<0.05).On down-regulating the expression of mRNA of VCAM-1