The voltage-dependent anion channel （VDAC）, a mitochondrial membrane channel protein located in the outer of mitochondrial membrane, is the main pathway between mitochondria and cytoplasm exchanging ADP, ATP, and other metabolites, and plays an important role in mitochondrial metabolism and cell growth. A growing evidence showed that VDAC was increased in cardiovascular diseases including myocardial ischemia and reperfusion, diabetes, heart failure, hypertension and atherosclerosis. The abnormal state of VDAC will result in cell death by inducing calcium cycling dysfunction and oxidative stress. And VDAC has become a hot topic in the field of cardiovascular diseases research. In this article, we will introduce the molecular function and regulation of VDAC and its role in cardiovascular diseases.