Aim To seek the association between plasma osteopontin （OPN） levels and severity of coronary heart disease in non-diabetic subjects. Methods A total of 166 stable angina patients free of diabetes were enrolled in the study. Clinical characteristic of patients was recorded. Plasma OPN levels were measured by an enzyme-linked immunosorbent assay method. Coronary heart disease was determined by coronary artery angiography. The extent of coronary artery stenosis was represented as the numbers of >50% stenotic vessels. Coronary artery clacification was evaluated by 64 slice spiral CT. The coronary artery clacification scores were classified into 4 categories on the basis of their severity: no calcification （≤10）, mild calcification （11～100）, moderate （101～400）, severe （> 400）. Results As the severity of coronary artery calcification and coronary stenosis, plasma OPN levels were increased. Plasma OPN levels were positively associated with coronary artery calcification and coronary stenosis. Conclusion Plasma OPN levels can be considered as an independent factor of coronary artery calcification and coronary stenosis in coronary heart disease patient without diabetes.