Aim To observe the expression of FLVCR and BCRP at different time points and effects of traditional herbal medicines NTE （ extract of HuangQi,Chuan Xiong,Di Long,Jiang Can） on FLVCR and BCRP after Focal Cerebral Ischemia in rats. Methods The experiment is carried out by two steps. Firstly , SD rats were randomly divided into 2 h, 6 h, 12 h, 24 h, 72 h groups, after operation of middle cerebral artery occlusion（MCAO）, FLVCR , BCRP and FLVCR -mRNA ,BCRP -mRNA expressions were detected by immunohistochemistry and RT-PCR just at 2 h, 6 h, 12 h, 24 h, 72 h time points.