Aim To investigate the association between polymorphism of Klotho G-395A and susceptibility of coronary heart disease （CHD） in East-Asia population. Methods PubMed, Embase, Cochrane Library, Sciencedirect, Springer, CBM disc, Wanfang database were searched for published case-control studies investigating the association between Klotho G-395A and CHD that were available before Dec.2013. Fixed or random effect models were selected for odds ratio （OR）calculation. A meta-analysis was performed to estimate heterogeneity and the pooled OR to evaluate the relationship between Klotho G-395A polymorphism and CHD. The sensitivity analysis was also assessed. Results A total of 6 case-control studies involving 1560 patients and 1459 controls were analyzed in the study. There were no significant heterogeneity found （dominant genetic model: P0.2, I²30.8%）. We observed a significant statistical association between Klotho G-395A polymorphism and CHD （dominant genetic model: OR1.24，95%CI: 1.06～1.45, P0.009）. After stratification by case selection criteria based on complete coronary angiographic data or not, no significant heterogeneity was observed in both groups. A stronger association was observed in the group which all the patients underwent coronary angiography（OR1.38，95%CI: 1.12～1.70, P0.003）, but negative association was found in the other group（OR1.07，95%CI：0.84～1.37,P0.587）. There was no significant heterogeneity among the remaining trial after using random effect model or excluding the trial with the largest weight or the trial with larger frequencies of the allele A, respectively. And the pooled OR （95%CI） value of the frequencies of the genotype（GA+AA）/GG were similar. Publication bias was not found by Begg’s test. Conclusion Klotho G-395A polymorphism may be a susceptible factor of CHD in East-Asia population.