Aim To explore whether adipophilin interact with acyl-coenzyme A:cholesterol acyltransferse 1 （ACAT1） and neutral cholesterol ester hydrolase （NCEH） in lipid-loaded RAW264.7 cell induced by oxidized low density lipoprotein （ox-LDL）.Methods RAW264.7 cells were incubated with 50 mg/L ox-LDL for different time.The expression of mRNA and proteins of adipophilin,ACAT1 and NCEH were detected by semi-quantitative reverse transcription-polymerase chain reaction and western blot respectively.Interactions between adipophilin and ACAT1 or NCEH were detected by co-immunoprecipitation.Results As the incubation time of ox-LDL was extended in RAW264.7 cells,the expression of mRNA and proteins of adipophilin,ACAT1 and NCEH were significantly increased compared with 0 h group （P<0.05,n＝3）.Co-immunoprecipitation showed that there were interactions between adipophilin and ACAT1 in RAW264.7 macrophages with ox-LDL treatment at 0,0.5,1 h and 3 h,and no interactions at 6 h.There were not interactions between adipophilin and NCEH in RAW264.7 macrophages with ox-LDL treatment at 0,0.5 h and 1 h,and interactions at 3 h and 6 h.Conclusions There were interactions between adipophilin and ACAT1 or NCEH in RAW264.7 macrophages with ox-LDL treatment.It suggests that adipophilin may have synergistic effects with ACAT1 and NCEH in lipid-loaded RAW264.7 cell.