Aim To explore the effect of probucol on function of diatetic mice adipose tissue-derived stem cells （ADSC）.Methods ADSC were isolated by mechanical separation and enzymatic digestion from nondiabetic and diabetic mice.The expression profiles of CD34,CD45,CD90 and CD105 were examined by flow cytometry.The ADSC were divided into 6 groups： control normal group,probucol normal group,control diabetic group,probucol diabetic group，high glucose normal group and probucol high glucose normal group.The proliferation and migration of ADSC was determined by WST-8 assay and transwell assay respectively.In addition,the mRNA and protein expression of vascular endothelial growth factor （VEGF）,hepatocyte growth factor （HGF）,and insulin-like growth factor-1（IGF-1） were determined by real-time PCR and ELISA analysis.The content of reactivated oxygen species （ROS） was also measured.Results The morphological feature of ADSC displayed fibroblast-like phenotype.The cells were positive for stem cells markers,including CD90 and CD105,and negative for CD34 and CD45,as shown by flow cytometry.Diabetic ADSC showed decreased proliferative potential and migration.In addition,the mRNA expression of VEGF,HGF and IGF-1 in diabetetic control group was obviously lower than that in nondiabetic control group.The contents of VEGF,HGF and IGF-1 on ADSC -conditioned medium in diabetic control group was also obviously lower than that in nondiabetic control group.Probucol promoted proliferation and migration of diabetic ADSC,and increase the mRNA expression of VEGF,HGF and IGF-1 in diabetetic ADSC.In addition,Probucol could increase contents of VEGF,HGF and IGF-1 in ADSC -conditioned medium in diabetic mice.Conclusion Our data indicate that diabetes alters ADSC intrinsic properties and impairs their function.Probucol can protect diabetic ADSC function of proliferation and migration,as well as releasing growth factors.