Aim To investigate the effect of profilin-1 on the process of endothelial cell damage mediated by advanced glycation end product(AGE)in cultured human umbilical vein endothelial cell(HUVEC）. Methods The endothelial cells were incubated by AGE with different concentrations(100, 200, 400 mg/L)and times(6, 12, 24, 48 h）. The protein expression of profilin-1 were determined by Western blot. The morphology and distribution of F-actin was detected by immunofluorescent staining. The levels of asymmetric dimethylargnine(ADMA）, intercellular adhesion molecule-1(ICAM-1）, nitric oxide(NO)in cultured condition and reactive oxygen species(ROS)were detected by related kits. Results Compared with control, profilin-1 protein expression was significantly up-regulated after treatment with 200 mg/L AGE for 24 h, concomitantly with the markedly increased levels of ICAM-1, ADMA and ROS and the significantly decreased levels of NO. Compared with control, the morphology and distribution of F-actin was significantly altered by AGE. Interfering with the profilin-1 gene expression can protect the extent of cell damage induced by AGE by down-regulating the protein expression of profilin-1, improving the distribution of F-actin, decreasing the levels of ICAM-1 and ADMA and increasing the levels of NO. Conclusion AGE induced endothelial cell damage by upregulating the expression of profilin-1