Aim To investigate the effect of β-nerve growth factor （β-NGF） gene transfected human umbilical vein endothelial cells （HUVEC） on cell function related to proliferation,monoclonal formation,cell cycle,apoptosis,migration. Methods The recombination human β-NGF gene expression eukaryotic plasmid with EGFP （pEGFP-N1） was transfected into HUVEC,HUVEC without transfected plasmid as the normal control group,HUVEC with empty vector （pEGFP-N1） as the negative control group,and-NGF gene transfection with the 1∶1000 K252a as the tyrosine receptor kinase A （TrkA） inhibition group.