Effects of Insulin-like Growth Factor Binding Protein-4 on Vascular Endothelial Cell Senescence
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    Abstract:

    Aim To investigate the effects of insulin-like growth factor binding protein-4 (IGFBP4) on vascular endothelial cell senescence.Methods Human umbilical vein endothelial cells (HUVEC) were cultured in vitro. A HUVEC aging model was established through cell passaging. Lentivirus expressing IGFBP4 or si-IGFBP4,and empty vector transfected HUVEC,then the effects of IGFBP4 on the aging process of these endothelial cells were studied by using light microscopy,senescence-associated (SA) β-galactosidase staining,and cell cycle analysis.Results After lentivirus transfection,the HUVEC passage number of the overexpressing IGFBP4(+) group (IGFBP(+) group) was higher than that of the empty vector group (control group) and IGFBP4 silenced groups (IGFBP4(-) group) (14.33±0.67 vs 9.67±0.33 and 10.67±0.33,P<0.05) The cell senescence rate of the IGFBP4(+) group was lower than that of the control group and IGFBP4(-) group in every passage (take the 6th passage for example,24.60%±0.90% vs 34.92%±0.92% and 33.74%±0.62%,P<0.05) Along with degenerate,the decreased cell proliferation index (△PI) from young passage (2nd passage) to old passage (6th passage) of IGFBP4(+) was smaller than control group and IGFBP4(-) group (10.35%±0.57% vs 14.90%±0.85% and 13.99%±0.65%,P<0.05).Conclusions IGFBP4 overexpression could inhibit HUVEC senescence.

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CHEN Chun-Yan, GUO Jin-Cun, and HUANG Wei-Bin. Effects of Insulin-like Growth Factor Binding Protein-4 on Vascular Endothelial Cell Senescence[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2015,23(05):490-494.

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  • Received:July 31,2014
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