Aim The aim of this study is to explore the correlation between serum vitamin D level and atherogenic index of plasma （AIP） in male.Methods From January to December in 2013,male 934 cases were selected in physical examination center of the 306th hospital of PLA. Fasting venous blood was collected from cases. Serum 25-hydroxy vitamin D ［25（OH）D］ level was determinated. Serum triglyceride and high density lipoprotein cholesterol were detected,and AIP was calculated. The relationship between 25（OH）D and AIP was analyzed.Results The average level of 25（OH）D and AIP in entire subjects were 52.23±21.59 nmol/L and 0.10±0.30 respectively. There was a negative correlation between the level of 25（OH）D and AIP （r＝－0.10,P＜0.01）. The value of AIP was significantly higher in vitamin D deficient group than that in vitamin D normal group （0.13±0.30 vs 0.08±0.29,P＜0.05）. The serum 25（OH）D concentration in the AIP abnormal group was significantly lower than that in the AIP normal group （51.35±21.06 nmol/L vs 54.66±21.90 nmol/L,P＜0.05）.Conclusions The serum vitamin D level is closely related to the AIP in male. Thereby,maintaining the body’s normal level of vitamin D may have benefits on the prevention of the atherosclerosis and atherosclerosis related diseases.