Aim To investigate the influences of 3 kinds of traditional Chinese herbal compound drug serum Sijunzi decoction （SD）, Xuefu Zhuyu decoction （XZD） and Buyang Huanwu decoction （BHD） on the expression of lectin-like oxidized low density lipoprotein receptor-1 （LOX-1）, tumor necrosis factor-α （TNF-α）, vascular cell adhesion molecule-1 （VCAM-1） and intercellular adhesion molecule-1 （ICAM-1） induced by lipopolysaccharide （LPS） in human umbilical vein endothelial cell （HUVEC）, and to explore the mechanism of traditional Chinese medicine compound preventing and treating atherosclerosis. Methods For cell experiment, SD, XZD, BHD containing drug serums and normal blank serum were prepared by using drug serological method. HUVECs were cultured in vitro and divided randomly into 6 groups: blank control group, model group, atorvastatin group, SD group, XZD group and BHD group. HUVECs were stimulated with LPS for 2 h, then treated separately with atorvastatin and traditional Chinese herbal compound drug serum for 24 h. The mRNA and protein expressions of LOX-1, TNF-α, VCAM-1, and ICAM-1 were detectd by fluorescence quantitative PCR and Western blot. Results mRNA and protein expressions of LOX-1, TNF-α, VCAM-1 and ICAM-1 were significantly increased after HUVEC stimulated by LPS （P<0.01）. mRNA and protein expressions of LOX-1, TNF-α, VCAM-1 and ICAM-1 were significantly inhibited, after adding respectively XZD, BHD containing drug serums and atorvastatin to intervene （P<0.05）. But SD containing drug serum had no significant effect （P>0.05）. Conclusions Traditional Chinese herbal compound XZD and BHD can inhibit the expressions of inflammatory factors LOX-1, TNF-α, VCAM-1 and ICAM-1, and this may be one of the mechanisms of them to prevent and treat atherosclerosis. While SD has no such role.