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All-trans Retinoic Acid Combined with Collagen Type Ⅳ Promoted Embryonic Stem Cells to the Differentiation of Smooth Muscle Cell
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1.Department of Cardiology, the Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550000, China;2.Department of Cardiology, General Hospital of Jinan Millitary Region of PLA, Jinan, Shandong 250000, China;3.Department of Cardiology, General Hospital of Shenyang Millitary Region of PLA, Shenyang, Liaoning 110016, China)

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R363

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    Abstract:

    Aim To investigate whether all-trans retinoic acid(atRA) combined with collagen Ⅳ can promote mouse embryonic stem cells(mESC)to differentiate into smooth muscle cell. Methods The totipotency and chromosome of ES cells were examined by ALP staining, SSEA-1 staining, and in-vivo teratoma formation test. After forming EBs by suspending in EB media for 3 days, EBs were divided into four inducted groups:none induction(Control group); atRA group; collagen Ⅳ group; atRA+collagenⅣgroup. They were differentiated for 7 d and 14 d. Then, they were harvested at 7 d and 14 d to analysie by Western blot and immunostaining. Results ①The successful culture of the ESCs was identified by the totipotent function. ②Compared with the other three groups, the expression of SM-α actin in group atRA+collagenⅣ significantly increased(P<0.05). Meanwhile, the proportion of SM-α actin+/SM-MHC+ cells in group atRA+collagen Ⅳ was higher than the other three groups. Conclusion atRA combined with collagen Ⅳ could do better in inducing the differentiation of mouse embryonic stem cells to smooth muscle cell than both alone.

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SHAO Xiao-Ping, PENG Cheng-Fei, TIAN Xiao-Xiang, YAN Cheng-Hui, HAN Ya-Ling, ZHANG Hong-Ming, LIU Da-Nan. All-trans Retinoic Acid Combined with Collagen Type Ⅳ Promoted Embryonic Stem Cells to the Differentiation of Smooth Muscle Cell[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2016,24(2):119-123.

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History
  • Received:June 04,2015
  • Revised:September 15,2015
  • Online: June 30,2016
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