Aim To investigate the value of serum vascular endothelial growth factor-B (VEGF-B) in the prognosis evaluation of patients with acute myocardial infarction(AMI). Methods Sixty-two patients with AMI were selected as case group in the department of cardiology from March 2014 to October 2015, and the elbow venous blood of AMI patients were immediately drawn before discharge, and enzyme-linked immunosorbent assay (ELISA) method was used for determining the concentrations of serum VEGF-B. The increased values (ΔEDV, ΔM) of the left ventricular end diastolic volume(LVEDV), left ventricular mass(LVM) and the occurrence of major adverse cardiac events(MACE) of the AMI patients were followed up within sixty days after discharge. The AMI patients were divided into ΔEDV≤0 group and ΔEDV>0 group, ΔM≤0 group and ΔM>0 group respectively according to the ΔEDV level and ΔM level. According to MACE occurrence, the AMI patients were divided into the MACE group and non-MACE group. The predictive value of VEGF-B concentration before discharge was analysed to left ventricular remodeling(LVR) and MACE after discharge. The relationship between MACE and LVR in AMI patients after discharge was also analysed. Results According to the results of the follow-up, the concentration of serum VEGF-B of ΔEDV≤0 group ((110.61±38.29) μg/L, n=34) was significantly higher than that of ΔEDV>0 group ((67.74±24.32) μg/L, n=26), the concentration of serum VEGF-B of ΔM≤0 group ((112.46±39.91) μg/L, n=32) was significantly higher than that of ΔM>0 group ((70.24±23.17) μg/L, n=28), the concentration of serum VEGF-B of the MACE group ((53.73±15.70) μg/L, n=25) was significantly lower than that of non-MACE group ((111.95±33.16) μg/L, n=37), the differences were statistically significant (P<0.01). The incidence of MACE in LVR group (100%) was higher than that of non-LVR group (5.26%), the difference was statistically significant (P<0.01). Conclusion For AMI patients, the lower concentration of serum VEGF-B before discharge, the more LVR obviously after discharge. Low level of VEGF-B predicts LVR after AMI. For AMI patients, the lower concentration of serum VEGF-B before discharge, the higher incidence of MACE after discharge. The concentration of serum VEGF-B before discharge can be used as a prediction index of MACE occurrence after discharge.