Aim To investigate the effect of microRNA-223 (miR-223) on apoptosis and oxidative stress of human adipose tissue-derived mesenchymal stem cell (hADSC) induced by advanced glycosylation end product (AGE). Methods The hADSCs were isolated and cultured by enzymatic digestion, and flow cytometry was used to detect the surface antigen (CD14, CD34, CD45, CD90, CD105 and HLA-DR) of hADSC. The hADSCs were divided into 6 groups:bovine serum albumin (BSA) group, AGE modified BSA (AGE-BSA) treatment group, miR-223 mimic transfection group, miR-223 mimic transfection＋AGE-BSA group, miR-223 inhibitor transfection group, and miR-223 inhibitor transfection＋AGE-BSA group. Cell viability and apoptosis rate were evaluated by CCK-8 assay and TUNEL assay respectively.Western blot was used to assess the expression of Cleaved Caspase3 protein in hADSC. The content of reactive oxygen species (ROS) in hADSC was determined by 2’,7’-dichlorfluorescein-diacetate (DCFH-DA) reagent kit. Results The hADSCs were positive for stem cell markers, including CD14, CD90 and CD105, and negative for CD34, CD45 and HLA-DR, as shown by flow cytometry. CCK-8 method, TUNEL staining, Western blot and DCFH-DA test results showed that:Compared with the BSA control group, the hADSC apoptosis rate, miR-223 expression, Cleaved Caspase3 protein expression and ROS production increased significantly in AGE-BSA treatment group, but hADSC survival rate decreased (P＜0.05); Compared with AGE-BSA treatment group, miR-223 mimic transfection could further increase the hADSC apoptosis rate, Cleaved Caspase3 protein expression and ROS production induced by AGE-BSA, and could further reduce hADSC survival rate (P＜0.05); Compared with AGE-BSA treatment group, miR-223 inhibitor transfection could inhibit the increases of hADSC apoptosis rate, Cleaved Caspase3 protein expression and ROS production, and could prevent the decrease of hADSC survival rate induced by AGE-BSA (P＜0.05). Conclusion High expression of miR-223 can promote the hADSC apoptosis and ROS production, and miR-223 may serve as a new target for the regulation of hADSC in promoting the healing of diabetic wound.