Aim To probe the effect of Intermittent aerobic exercise on Adrenomedullin (ADM) secretion of artery of spontaneous hypertensive rat (SHR), and investigate the mechanism of artery of exercise in the prevention and treatment of hypertension. Methods 30 SHR are separated into two groups:intermittent exercise group (IAE Group) and control group. The IAE group performs 8 weeks intermittent treadmill exercise and their blood pressure are measured before and after exercise by tail pressure of SHR, serum ADM is detected by enzyme linked immunosorbent assay. ADM is expressed in artery by immunohistochemistry. Quantification of specific-receptor activity modifying protein (RAMP2) and calcitonin receptor like receptor(CRLR) in artery ADM are detected by Westernblotting. Results Quantification of specific receptor ADM were significantly lower than control group(P<0.05) and serum ADM in IAE group were significantly higher than control group(P<0.05). The expression of ADM in artery was obviously stronger than control group. The quantity of RAMP2 and CRLR in artery were increased obviously (P<0.05). Conclusion Intermittent aerobic exercise increases the expression of ADM in artery and their specific receptor RAMP2 and CRLR up regulation may be one of the mechanisms of lower SHR blood pressure.