Establishing methods for studying intravascular lipid metabolism of zebrafish

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Establishing methods for studying intravascular lipid metabolism of zebrafish
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                        CHEN KanCHEN Kan
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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WANG Chang-QianWANG Chang-Qian
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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FAN Yu-QiFAN Yu-Qi
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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HAN Zhi-HuaHAN Zhi-Hua
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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WANG YueWANG Yue
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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GAO LinGAO Lin
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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ZENG Hua-SuZENG Hua-Su
Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China
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Affiliation:Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China)
Clc Number:R541.4
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Abstract:

Aim To establish systematic methods for studying intravascular lipid metabolism of zebrafish. Methods Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting were used to study intravascular lipid metabolism of embryo, larvae and adult zebrafish and the effect of different diet on blood lipid of zebrafish. Results Intravascular lipid of embryo zebrafish was stained by oil red O staining, and intravascular cholesterol was labeled by CholEsteryl BODIPY 542/563 C11. Increased intravascular lipid of larvae zebrafish incubated in egg yolk was stained by oil red O staining, and increased intravascular cholesterol of larvae zebrafish fed by high cholesterol diet was labeled by CholEsteryl BODIPY 542/563 C11. The serum total cholesterol and triglyceride of adult zebrafish were obviously increased after 7 weeks fat feeding. Conclusion Oil red O staining, CholEsteryl BODIPY 542/563 C11 fluorescently labelling and blood lipids detecting can be applied for zebrafish intravascular lipid metabolism research.

Key words:Zebrafish; Lipid metabolism; Oil red O staining; Fluorescent probe labeling

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CHEN Kan, WANG Chang-Qian, FAN Yu-Qi, HAN Zhi-Hua, WANG Yue, GAO Lin, ZENG Hua-Su. Establishing methods for studying intravascular lipid metabolism of zebrafish[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2017,25(4):398-403.

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Received:July 27,2016
Revised:December 08,2016
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Online: May 18,2017
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