Adipose tissue lipoprotein lipase (LPL) is regulated by angiopoietin-like protein and influences the storage and energy supply of adipose energy. Myocardial LPL is regulated by peroxisome proliferator-activated receptor and adenosine monophosphate activated protein kinase and influences the uptake of free fatty acids in the absence of cardiac energy supply. Macrophage LPL is regulated by microRNA and inhibits the formation of foam cells. The regulation of LPL in various tissues is influenced by many factors, and the effects are different. In this paper, we reviewed the regulation of LPL in adipose tissue, myocardium and macrophages, and the effects of related proteins on LPL activity, in order to further clarify the role and significance of LPL in various tissues.