Aim To prepare the no-reflow model after myocardial ischemia-reperfusion injury in rats and to explore the key points in the establishment of the model, so as to provide reference for the successful replication of the disease model. Methods Twenty SD rats were randomly divided into no-reflow group (n＝15) and sham group (n＝5). In the no-reflow group, the left anterior descending artery (LAD) was ligated 45 min and then perfused 120 min. The sham group rats underwent the same surgical procedures except that LAD was not ligated. At the end of reperfusion, thioflavin S staining was performed to determine the area of no-reflow (ANR), while monitoring the changes of respiration and electrocardiogram (ECG) in rats. Results Twenty rats were all survived, at the selected condition of ischemia and reperfusion time, all the rats in the no-reflow group showed obviously myocardial no-reflow phenomenon. After no-reflow model was established, the heart rate accelerated and ST segment elevation increased in the ischemic stage. Along with a progress of reperfusion, the heart rate and amplitude increased first and then decreased, while the increase values of ST segment drop less than 30%. Conclusion The method can successfully replicate the animal model of no-reflow and the method has high success rate and high animal survival rate.