2025年4月18日 周五
Home > Archive>Volume 26, Issue 11, 2018 >1099-1105
PDF HTML XML Export Cite reminder
The effect of interplay between endoplasmic reticulum stress and autophagy on vascular calcification
CSTR:
[cstr]
Author:
Affiliation:

1.Department of Physiology, Hebei Medical University, Shijiazhuang, Hebei 050017;2.Department of Gynecology, Hebei Hospital of Chinese Medicine, Shijiazhuang, Hebei 050011;3.Clinical Medicine Major, Hebei Medical University, Shijiazhuang, Hebei 050017;4.Public Health Major, Hebei Medical University, Shijiazhuang, Hebei 050017;5.Hebei Key Lab of Laboratory Animal Science, Hebei Medical University, Shijiazhuang, Hebei 050017, China)

Clc Number:

R363

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    Aim To determine the effect of interplay between endoplasmic reticulum stress (ERS) and autophagy on vascular calcification (VC). Methods VC model in rats was induced by vitamin D3 plus nicotine. The deposition of calcium in aorta was determined by alizarin red staining and assessment of calcium content. The protein levels in aorta were measured by Western blot. Results Compared with control group, deposition of calcium in aorta was increased in VC group. The protein levels of SM-22α and calponin in calcified vessels were decreased, whereas that of bone morphogenetic protein 2 (BMP-2) and RUNX2 was increased. ERS and autophagy was activated in calcified aorta, shown by increased levels of glucose regulated protein (GRP78), C/EBP homogous protein (CHOP), LC3Ⅱ and Beclin-1. Tunicamycin (10 μg/kg per day), an ERS inducer exacerbated VC and transdifferation of vascular smooth muscle cells from contracted-like to osteoblasts-like phenotype. It also promoted ERS and auphagy. 3-methyladenine (10 mg/kg per day), an autophagy inhibitor prevented stimulation of autophagy, but promoted ERS activation in calcified aorta, while it also exaggerated VC and transdifferation of vascular smooth muscle cells. Conclusion The interplay between ERS and autophagy plays an important role in progression of VC.

    Reference
    Related
    Cited by
Get Citation

LI Yanqing, FU Jia, LI Tong, REN Yangyang, XU Yixin, XIN Qingjie, DONG Jianlong, YANG Rui, WU Yuming, TENG Xu. The effect of interplay between endoplasmic reticulum stress and autophagy on vascular calcification[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2018,26(11):1099-1105.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 15,2018
  • Revised:September 20,2018
  • Online: December 11,2018
Article QR Code

You are the visitor

Post Code：421001 Fax：0734-8160523

Phone：0734-8160765 E-mail：dmzzbjb@163.net

Editorial Office of Chinese Journal of Arteriosclerosis ® 2025