Aim To investigate the effects and mechanisms of quantum lipid-lowering instrument on lipid regulation. Methods High fat diet and 10% fructose were used to establish a rat model of hyperlipidemia. The output power of the quantum lipid-lowering instrument was 70 μW/cm², and the treatment time was 5~10 minutes for each time,1~2 times a day. Elisa kit was used to detect serum lipid levels and Western blot was used to measure the expressions of key protein in lipid metabolic and inflammatory pathways. Results Quantum lipid-lowering instrument can improve the disorder of blood lipid metabolism, regulate blood lipid balance, reduce inflammation, alleviate liver function damage, alleviate oxidative damage, increase the activity of lipid metabolism-related enzymes, and inhibit the activity of fatty acid synthase which is caused by hyperlipidemia. Western blot showed that Quantum lipid-lowering instrument could alleviate inflammation by regulating the expression levels of nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6);modulate the expression of peroxisome proliferators-activated receptors α (PPARα) which could improve lipid metabolism;regulate the sterol-regulatory element binding proteins 2 (SREBP-2) pathway to increase low-density lipoprotein receptor (LDLR) protein expression, alleviate lipid metabolism disorders, and regulate cholesterol and triglyceride levels. Conclusion Quantum lipid-lowering instrument has obvious effect of lipid regulation, and can be used as an assisted clinical instrument for lowering lipid.