Aim To observe the effects of components of Xiongshao capsule on cholesterol and inflammatory factors on foam cells in vitro. Methods Oxidized low-density lipoprotein (ox-LDL) was used to induce the foaming of RAW264.7 cells. The proliferation activity of foam cells was detected by CCK-8 reagent. The total cholesterol (TC) and free cholesterol (FC) in foam cells were detected by cholesterol test kit, and the lipid distribution in cells was observed by oil red staining. The concentration of inflammatory factors, tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) was detected by ELISA kit. Results The results of CCK-8 showed that ligustrazine and paeoniflorin had no obvious effect on the proliferation of foam cells under 80 mg/L concentration. 40 mg/L ligustrazine plus 80 mg/L paeoniflorin could significantly reduce TC and FC contents in foam cells, reduce lipid deposition in foam cells, and inhibit the secretion of TNF-α and IL-1β by foam cells. Conclusion It is proved that the effective components of Xiongshao capsule, ligustrazine and paeoniflorin, can reduce the cholesterol content in foam cells derived from RAW264.7 cells and reduce the inflammatory factors, TNF-α and IL-1β produced by foam cells which have an potential ability to inhibit inflammatory reaction.