Aim To explore the effects of Guanxinkang on myocardial ischemia/reperfusion injury (MI/RI) in rats with hyperlipidemia. Methods SD male rats were randomly divided into three groups:sham operation group (10 rats), model group (10 rats) and Guanxinkang group (10 rats). The hyperlipidemia rat model was established by high fat, and the MI/RI model was established by reversible left anterior descending artery ligation and given drug intervention.The biochemical analysis method was used to detect the blood lipids levels after intervention. The blood viscosity in each group was detected by viscometer. The serum levels of interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1) and malondialdehyde (MDA) were detected by ELISA. Caspase-3 activity was detected by fluorescence method, and cardiomyocyte apoptosis rate was detected by TUNEL assay, and the changes of myocardial tissues were observed by masson staining and HE staining, and the levels of myocardial apoptosis-related proteins were detected by Western blot. Results Compared with sham operation group, the triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDLC), whole blood viscosity, plasma viscosity, TNF-α, MDA, ICAM-1, apoptosis rate, caspase-3 activity and Bax protein were increased while the levels of high density lipoprotein cholesterol (HDLC), IL-10 and Bcl-2 protein were decreased in model group, and myocardial structure was disordered and the damage degree was severe. Compared with model group, the TG, TC, LDLC, whole blood viscosity, plasma viscosity, TNF-α, MDA, ICAM-1, apoptosis rate, caspase-3 activity and Bax protein level were decreased while the levels of HDLC, IL-10 and Bcl-2 protein were increased in Guanxinkang group, and the rat myocardial structure was relatively neat and the damage degree was low. Conclusion Guanxinkang has protective effects on myocardial function in rats with hyperlipidemia and myocardial ischemia/reperfusion injury, which may be related to lowering blood lipids levels and inflammatory reactions and inhibiting cardiomyocyte apoptosis.