Aim To explore the value of serum NLR, UCH-L1 and ABCD2 in the diagnosis of cerebral infarction in patients with isolated vertigo. Methods 258 patients with isolated vertigo were enrolled. According to the results, the patients were divided into 130 patients with cerebral infarction and 128 patients without cerebral infarction. The clinical data of the patients, ABCD2 scores, serum UCH-L1, NLR and imaging examinations were evaluated and compared between two groups. Logistic regression model was employed to produce new variables for diagnosis of cerebral infarction and assessed with ROC curve. Results ABCD2, UCH-L1 and NLR in cerebral infarction group were significantly higher than those in non-cerebral infarction group (P<0.05). Taking cerebral infarction as the dependent variable and ABCD2, UCH-L1, NLR as the independent variables, Logistic regression model was established, and ROC curve of combined diagnosis was fitted by the probability value in the model. ABCD2 (OR=1.1,5%CI:1.118～3.098), UCH-L1 (OR=1.7,5%CI:1.012～1.563) and NLR (OR=1.5,5%CI:1.005～1.149) were the risk factors of cerebral infarction in patients with isolated vertigo. ROC results showed that the critical value of ABCD2, UCH-L1 and NLR in the diagnosis of cerebral infarction was 3 points, 0.19 μg/L, 2.01, the area under ROC curve was 0.706 (0.642～0.771), 0.603 (0.533～0.672), 0.633 (0.566～0.700), the sensitivity was 0.2,0.1,0.603, and the specificity was 0.4,0.1,0.621. While the Logistic combination prediction module showed the AUC was 0.744 (0.681～0.807), the sensitivity was 0.861 and the specificity was 0.602. The sensitivity was higher than that of single diagnosis. Conclusion UCH-L1 and NLR combined with ABCD2 presents potential clinical evaluation value in the diagnosis of cerebral infarction in patients with isolated vertigo.