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Changes of plasma soluble Sema4D levels during cardiopulmonary bypass
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1.Cyrus Tang Hematology Center of Soochow University, Suzhou, Jiangsu 215123, China;2.The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China;3.Institute for Cardiovascular Science of Soochow University, Suzhou, Jiangsu 215006, China;4.Jilin Hospital of Chinese Armed Police Force, Changchun, Jilin 130000, China)

Clc Number:

R654.1

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    Abstract:

    Aim To measure and analyze the changes of plasma soluble Sema4D levels during cardiopulmonary bypass(CPB)with ELISA, and analyze possible mechanisms. Methods Recombinant extracellular Sema4D was used as antigen, and antibodies against Sema4D were used to establish ELISA method. Samples from patients during CPB were collected at three time points: before surgery, 10 min after heparinization, and 10 min after neutralization after the surgery.Soluble Sema4D levels in the plasma were measured in these samples with ELISA. Sema4D expression on the surface of T cells and platelets were detected by flow cytometry. Results Sandwich ELISA method was established to detect soluble Sema4D levels in human plasma. Soluble Sema4D levels increased during CPB and remained at high levels until the end of the surgery(P＜0.01). Compared with before surgery, percentage of Sema4D high platelets increased 10 min after heparinization in 75% patients(P＜0.05). There is no significant change of Sema4D expression on the surface of T cells during CPB.Conclusions Platelets activation and release of Sema4D may be one source of the increases of plasma soluble Sema4D during CPB. The change of plasma soluble Sema4D levels may become a prognostic indicator of CPB.

    Reference
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LI Ling, DING Yinglong, WANG Yuxin, XU Linru, YI Wenxiu, SHEN Zhenya, ZHU Li, LU Qiongyu. Changes of plasma soluble Sema4D levels during cardiopulmonary bypass[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2020,28(9):767-772.

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History
  • Received:April 15,2019
  • Revised:April 10,2020
  • Online: October 28,2020
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