Aim The purpose of this study was to observe the effect and mechanism of angiotensin(1-7)(Ang(1-7)) on angiotensin Ⅱ(AngⅡ)-induced senescence of human umbilical vein endothelial cell(HUVEC). MethodsHUVEC were cultured in vitro with DMEM high glucose medium containing 10%fetal bovine serum (FBS) for 48 hours and randomly divided into control group, Ang(1-7) group (1 μmol/L), AngⅡ group (1 μmol/L), and AngⅡ＋Ang(1-7) group. Cell senescence β-galactosidase(SA-β-Gal) staining kits were used to detect the number of senescent cell in each group (observed under an optical microscope); reactive oxygen species (ROS) levels were measured using reactive oxygen detection kits, and p53 and dynamin related protein-1(Drp1) expression in each group were detected by Western blot.Results Compared with the control group, both SA-β-Galpositive cell and ROS levels in the AngⅡ group were significantly increased(P＜0.001), and p53 and Drp1 protein expression in the cell were significantly increased (P＜0.01). Compared with AngⅡ group, SA-β-Galpositive cell(P＜0.01), ROS levels(P＜0.001), p53 and Drp1 protein expression(P＜0.05) were decreased in AngⅡ＋Ang(1-7) group. Conclusion Ang(1-7) may inhibit the ROS generation in HUVEC by affecting the p53/Drp1 pathway, and reverse the aging of HUVEC induced by AngⅡ.