Aim To explore the effect of regional collaborative treatment network on the treatment efficiency of acute ischemic stroke (AIS). Methods With network information as the core and 120 first aid as the link, the AIS regional collaborative treatment network of 8 community hospitals under the jurisdiction of Affiliated Hospital of Jiangsu University was established. The changes in AIS treatment efficiency, treatment effects and economic indicators before the implementation of the regional collaborative treatment network (pre-implementation group, 180 cases) and after implementation (post-implementation group, 218 cases) were compared. Results Compared with the pre-implementation group, the proportion of intravenous thrombolysis in the time window of AIS patients in the post-implementation group was significantly increased (31.2% vs. 2.8%, P＜0.05), and the time from admission to intravenous thrombolysis (door-to-needle time, DNT) was significantly shortened [(58.7±11.3) min vs. (101.6±14.5) min, P＜0.05], the proportion of head CT completed within 45 min of emergency was significantly increased (61.9% vs. 32.8%, P＜0.05). The mortality rate during hospitalization was lower in the post-implementation group than the pre-implementation group (5.5% vs. 8.3%, P＜0.05). Compared with the pre-implementation group, the number of hospitalization days in the post-implementation group was significantly shortened, and hospitalization expenses were significantly reduced (P＜0.05). Compared with the pre-implementation group, the daily living ability score (Barthel index) after 1 month in the post-implementation group was significantly increased, and the NIHSS score was significantly decreased (P＜0.05). Conclusion The implementation of regional coordinated treatment network can significantly improve the treatment efficiency of AIS, improve the clinical prognosis of patients, and reduce the economic burden. It is an effective measure to enhance the treatment ability of AIS.