Aim To observe the effect of sphingosine 1-phosphate receptor 1(S1PR1) on vascular smooth muscle cell proliferation and migration induced by advanced glycation end products (AGE). Methods Human umbilical artery smooth muscle cells (HUASMC) were cultured, and AGE-BSA was obtained by incubating with glucose and albumin. Cells were divided to control, BSA and AGE-BSA treated group. CCK-8 kit was used to detect the proliferation of smooth muscle cells, cell scratching and Transwell assays were applied to study the migration of smooth muscle cells. S1PR1 antagonist VPC23019 or agonist SEW2871 were used to investigate the role of S1PR1 in the proliferation and migration of human umbilical artery smooth muscle cells with BSA or AGE-BSA treatment. Results Compared with control group, BSA and AGE-BSA induced proliferation and migration of human umbilical artery smooth muscle cells, and the effect of AGE-BSA was more significant than that of BSA (P＜0.05). VPC23019, an antagonist of S1PR1, significantly attenuated the proliferation and migration of human umbilical artery smooth muscle cells induced by BSA and AGE-BSA. The S1PR1 agonist SEW2871 itself promoted the proliferation and migration of human umbilical artery smooth muscle cells, and further enhanced the proliferation and migration of human umbilical artery smooth muscle cells induced by BSA, but had no further promotion effect on the proliferation and migration of human umbilical artery smooth muscle cells induced by AGE-BSA. Conclusions Plasma albumin itself can promote the proliferation and migration of smooth muscle cells, and the effect of glycosylated albumin is more remarkable. The activation of S1PR1 is involved in BSA and AGE-BSA-induced proliferation and migration of human umbilical artery smooth muscle cells, and the activation of S1PR1 by AGE-BSA was more significant.