Extracellular vesicles(EV) are nano-sized lipid bilayer vesicles, which were released by every cell type. EV can transfer DNA, RNA and protein among cells, which is a key part of signaling transduction among cells. microRNA is a small non-coding RNA molecular, which can bind to 3′UTR sequence of targeted mRNA, and facilitate mRNA degradation or block protein generation. This study focuses on the generation of microRNA and how EV transfer microRNA among cells, as well as the function of EV-miRNA in cardiovascular diseases.