Abstract:Aim To investigate the characteristic of chloride currents and Ca 2+ entry induced by adrenaline (Adr) in A10 vascular smooth muscle cells (VSMC). Methods Cell attached patch clamp and Fura 2 fluorescence techniques were used to observe the chloride currents and the changes in [Ca 2+ ]i. Results The open probability (Po)and mean open time(τo) of chloride channels were increased by 10 μmol/L Adr; the Po were increased from 0.061 to 0.69; the τo were increased from 1.08 ms to 6.44 ms. The activatoion of chloride channels were decreased by 10 μmol/L nifedipine and nearly completely inhibited by subsequent addition of 1.5 mmol/L EGTA. Application of Adr activated Ca 2+ influx from the extracellular space. Both of the chloride currents and Ca 2+ influx could be inhibited by the chloride channels blockers niflumic acid (NFA) in a concentration dependent manner. Conclusion The Adr can increase the activation of chloride channels in VSMC and the action is dependent on the extracelluar Ca 2+ influx. The chloride channels play an important role in the regulation of Calcium movement in VSMCs.

Abstract:Aim To study the inhibitory action of Oyster Extract (OE) on atherosderosis and it's mechanism. Methods The model of experimental atherosclerosis in quail was established by hyper lipoidal feed. The content of serum lipid and apolipoprotein was detected with enzyme method and immunization photoextinction. The atherosclerotic plaque lesion of aortic and coronary artery were observed by eyes and through light microscope.The serum superoxide dismutase (SOD) and malondiadehyde (MDA) in quail were observed with microcontent fast detecting method. Results Oral administration OE 10 g/(kg·d) for an 8 weeks period significantly reduced the degree of atherosclerotic lesion in aortic and coronary arterious endothelium in OE group. The serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDLC) and apolipoprotein B (Apo B) are 17.47±0.78, 1.16±0.35, 10.94±3.17 mmol/L and 2.47±0.78 g/L respectively in OE group at 4 weeks,and were less than model group (p<0.05,p<0.01). At 8 weeks OE marked reduced the TC, TG, LDLC, and ApoB in serum and TC, TG in cardial muscle and aorta (p<0.05,p<0.01). At the same time and serum SOD in serum was 415.55±72.48 k u/L in OE group.It was higher than model group (p<0.05). Conclusions OE has an inhibitory effect on the development of experimental atherosclerosis lesions in quails, the mechanism may be related to regulation of lipid metabilism and antioxidation action.

Abstract:Aim To investigate whether homocysteine (HCY) could induce human umbilical vein endothelial cells (hUVEC) to express macrophage inflammatory protein 1α (MIP 1α). Methods After exposure of the cultured hUVEC to HCY either at a same concentration for different incubation time or at increasing concentrations but for a same incubation time, the MIP 1α mRNA and protein expression in the cells were determined by reverse transcriptase polymerase chain reaction (RT PCR) and immunocytochemistry, respectively. Results Cultured hUVEC could express MIP 1α mRNA and protein. RT PCR showed that exposure of hUVEC to HCY at a concentration of 0.1 mmol/L for 4, 8 and 16 h, resulted in a 2.08 fold, a 3.26 fold and a 3.35 fold increase in the expression of MIP 1α mRNA in hUVEC, compared with the control group; meanwhile, exposure of hUVEC to HCY at different concentrations (0.1 mmol/L, 0.5 mmol/L and 1 mmol/L) for 8 h, resulted in a 2.08 fold, a 4.35 fold and a 4.57 fold increase in the expression of MIP 1α mRNA in hUVEC as much as the control group, respectively. Immunocytochemistry showed that the absorbance values of MIP 1α protein expression in hUVEC exposed to HCY at a same concentration but for different incubation time mentioned above were 0.071±0.006, 0.081±0.006 and 0.128±0.009 (control group 0.049±0.005), respectively. There was a significantly statistical difference between groups (F=946.70, p<0.01). The absorbance values of MIP 1α protein in hUVEC exposed to HCY at different concentrations but for a same incubation time mentioned above were 0.081±0.006, 0.110±0.009 and 0.118±0.008 (control group 0.049±0.005), respectively, which also showeol significantly statistical difference between groups (F=546.89, p<0.01). Conclusion HCY was able to induce a high expression of MIP 1α mRNA and protein in cultured hUVEC.

Abstract:Aim To explore the existence of specific oxidized low density lipoprotein(ox LDL) receptor and assay its molecular weight. Method Cross competition studies and ligand blotting were performed for analyzing specific ox LDL receptor. Results As an effective competitor, acetyl LDL(ac LDL) could block the uptake of nonspecific ox LDL. Studies indicated that excess ac LDL(200 mg/L)could only inhibited 24.8% of the association and 77.1% of the degradation of ox LDL. Ligand blotting studies showed that 125 I ox LDL could bind to a protein of 84～97 kDa. The binding of ox LDL to this membrane protein was inhibited completely by the addition of excess unlabeled ox LDL. In contrast, 125 I ac LDL couldn't bind to the 84～97 kDa band. Conclusion Studies showed the existence of specific ox LDL receptor and the molecular weight of this receptor was 84～97 kDa.

Abstract:Aim To observe whether lipid peroxidation injury to endothelial cells (EC) induces intercellular adhesion molecule 1(ICAM 1) expression. Methods The lipid peroxidation injury to cultured human umbilical vein endothelial cells (hUVEC) was induced by exposure of the cells to diamide either at a same concentration for different incubation time or at different concentrations, but for a same incubation times. The ICAM 1 mRNA expression in hUVEC was determined by reverse transcriptase polymerase chain reaction (RT PCR), and was normalized with β actin mRNA. The ICAM 1 protein expression in hUVEC was determined by cell enzyme linked immunosorbent essy (ELISA). Results RT PCR showed that the ratios of the absorbance (A) values of ICAM 1 mRNA to that of the β actin mRNA expressed in hUVEC exposed to diamide at different concentrations (1 μmol/L, 5 μmol/L and 10 μmol/L) for 8 h was 0.535, 0.908 and 1.186, respectively. Whereas the ratio of the A values of ICAM 1 mRNA to that of the β actin mRNA expressed in hUVEC exposed to diamide at a concentration of 5 μmol/L for 0, 4 h and 24 h was 0.185, 0.598 and 1.292, respectively. Cell ELISA showed that the A values of ICAM 1 protein expressed in hUVEC exposed to diamide at different concentrations (1 μmol/L, 5 μmol/L and 10 μmol/L) for 4 h were 0.1387, 0.1775 and 0.2326, which were 1.34 fold, 1.71 fold and 2.25 fold as much as that of the control group (0.1035), respectively. There was a significant statistical difference between groups (F=38.89, p<0.01). Taken together, these results suggest that ICAM 1 expression in hUVEC induced by diamide was in a dose and time dependent manner. Conclusions Lipid peroxidation injury to EC induced by diamide may up regulate ICAM 1 mRNA and protein expression, which play an important role in the recruitment of monocytes into subendothelial space in atherogenesis.

Abstract:Aim To determine whether the extract of Buyang huanwu decoction(BHD)inhibit the pathogenetic progress of atherosclerosis induced by cholesrol rich diet and to elucidate its mechanism in rabbits. Methods Three groups of rabbits received the following different diets for 9 weeks: ordinary diet(OD) group, model of atherosclerosis (MA) group(ordinary diet plus 1% cholesterol and 3.3% fat), and MA plus BHD [5 g/(kg·d)] group. Plasma lipids, endothelin 1(ET 1), nitric oxide(NO) and factor Ⅶ procoagulant activity(FⅦ:C) were detected and the histological atherosclerotic changes in aortic, abdominal aortic and coronary artery were evaluated. Results Compared with the MA group, serum total cholesterol(12.70±6.17 vs 26.6±10.41 mmol/L, p<0.01), triglycerides (1.05±0.25 vs 2.72±1.55 mmol/L, p<0.01), FⅦ:C(135.36±34.54 vs 287.84±66.87, p<0.01), and NO (30.23±12.91 vs 112.30±23.00, p<0.01) were significantly lower in the MA plus BHD group, and this decoction inhibited theprogression of aortic, abdominal aortic and coronary intimal plaques and reducedaortic intimal thickening. Conclusions These datas suggested that BHD have antiatherogenic effects and its mechanism might associate with the decrease of plasma cholesterol, triglycerides, nitric oxide and factor Ⅶ procoagulant activity. (① A kind of traditional Chinese decoction consisted of seven kinds of Chinese medicine: astragalus; angelica; ligusticum; paeonia; earthworm; swietenia and carthamus.)

Abstract:Aim To investigate the responseness of the fibroblasts from the myocardium infarcted rats to angiotensinⅡ(AngⅡ). Methods With the tissue isolation, cell culture and the uptake rates of 3 H thymidine ( 3 H TdR), 14 C uridine ( 14 C UR) and 3 H proline ( 3 H Pro) representing syntheses of DNA,RNA and collagen protein of isolated fibroblasts (Fbs) from the heart of myocardial infarction (MI) rats and sham operated (SO) which were treated with losartan or captopril for 6 weeks, respectively. Results The uptake rates of 3 H TdR, 14 C UR and 3 H Pro of Fbs from MI group treated with losartan or captopril are not significantly different from those of the SO one, respectively. These effects of AngⅡon Fbs are abolished completely by 1.8 AngⅡand angiotensin antipeptide, but not completely by angiotensin receptor 1 antagonist, losartan. Conclusion The findings suggest that the responsiveness of Fbs to AngⅡ from MI rats treated with either losartan or captopril is significantly lower than that from that from the MI rats. AngⅡ acts on the Fbs from normal or hypertrophied myocardium via not only AT1 receptor, but also other mechanisms involving in the effects of AngⅡ to Fbs.

Abstract:Aim To establish a human umbilical vein endothelial cell (hUVEC) line monoclonal cells with the stable expression of vascular endothelial growth factor (VEGF) gene. Methods The eukaryotic expression vector PCD 2 VEGF 121 were transfected into hUVEC line cells mediated by lipofect AMINE. The positive clones were obtained by the screen of G 418 . The transcription and expression of VEGF gene were investigated by RT PCR and immunocytochemistry respectively. The experiment of Miles was applied for the assay of the biological activity of the protein of the VEGF produced by the hUVEC line cells which transfected PCD 2 VEGF 121 . The growth curve was made for comparison with that of non transfected hUVEC line cells. Results A positive clone cells from which transcripted the MRNA of VEGF 121 gene was obtained by RT PCR; The positive results of the immunocytochemistry were found and the highly biological activity of VEGF in the media was detected each only in the positive clone cells. The doubling time of the positive colon cells was shorter than that of the non transfected hUVEC line cells calculated from the growth curve. Conclusion The hUVEC line monoclonal cells with the stable expression of VEGF gene has been established successfully.

Abstract:Aim Investigate the Protective Effect of Shou-Gong Shui-Zhi Composition on Regional Cerebral Ischemia Reperfution Injury in Rats. Methods Acute cerebral ischimia reperfution injury model in the rat was established by introducing nylon suture into middle cerebral artery (MCA) for occlusion and then taking out the nylon suture. The behavior obstacle marks of rats was studded, and the water contents in brain,LDH and CK activity in serum,Na +, K +,Ca 2+ , Mg 2+ levels of cerebral tissue was measured. Results Shou-Gong Shui-Zhi Composition can enhance the behavior obstacle marks of rats, decrease contents of H 2O, LDH, CK activity and Na + levels after ischemia reperfution. Conclusion Shou-Gong Shui-Zhi Composition could protect brain ischemia reperfution injury

Abstract:Aim To investigate the preventive effects of polysaccharide krestin (PSK) on myocardial ischemia reperfusion (I/R) injury. Methods Eight mongrel dogs of male, anesthetized with sodium pentobarbital, underwent 60 min of left anterior descending coronary artery occlusion, followed by 120 min reperfusion (I/R group). Another six dogs (PSK group) were subjected to the same ischemia reperfusion as I/R group, received PSK, which was administered orally, at a dose of 150 mg/kg once daily for two days before thoracotomy. Left ventricular function was evaluated by echocardiography using an echocardiogram and with measurement of left ventricular diastolic pressure (LVDP) by catheterization, and blood samples were taken from coronary venous sinus for the examination of malondialdehyde (MDA), at different time points. The ischemia reperfusion myocardium was examined pathologically. Results LVDP markedly rose before and in early reperfusion in I/R group, but only prior to reperfusion in PSK group. The systolic thickening of ischemia reperfusion myocardium region and left ventricular ejection fraction markedly declined during ischemia in both groups, but progressively improved with the time of reperfusion. In PSK group, the recovery of wall thickening was greater than in I/R group during reperfusion. Plasma MDA concentration during reperfusion in I/R group was higher than its baseline. In PSK group, MDA concentration rose only in early reperfusion, but recovered rapidly. In I/R group, myocardial cellular edema were present and, the fractures of a few myofilaments, the granule loss and swelling of mitochondrias were also seen. The ultrastructural abnormalities were much slighter in PSK group. Conclusion PSK could greatly alleviate the functional impairment and structural abnormalities of ischemia reperfusion myocardium.

Abstract:Aim To explore the cardioprotection mechanism of red wine and resveratrol, the effects of red wine and resveratrol on platelet aggregation both in vitro and in vivo were studied. Methods Hypercholesterolemia in rabbits was induced by high cholesterol diet. Platelet aggregation induced by thrombin, ADP and collagen was measured by using Born's method. Results Hypercholesterolemia induced by high cholesterol diet enhanced the platelet aggregation in rabbits. Red wine, de alcohol red wine or resveratrol were administered orally, which significantly inhibited ADP induced platelet aggregation in rabbits with hypercholesterolemia. In vitro resveratrol significantly inhibited healthy volunteers' platelet aggregation induced by thrombin, ADP or collagen in a concentration dependent manner. Conclusions Red wine and resveratrol can inhibit platelet aggregation both in vitro and in vivo. This may be one of the mechanisms by which resveratrol prevents against atherosclerosis.

Abstract:Aim To study the effects of oxidized high density lipoprotein (ox HDL) on the migration of THP 1 cells and monocyte chemoattractant protein 1 (MCP 1)in the conditioned medium. Methods Human plasma lipoproteins were separated by one step ultracentrifugation with a simple density gradient, the in vitro oxidation of high density lipoprotein (HDL) was mediated by CuSO 4. THP 1 cells (a human acute monocytic leukemia cell line) were cultured for the experiments. The migration of THP 1 cells stimulated by oxidized high density lipoprotein was assayed with reformed Boyden. Results The migrated cells in the membrane (in Boyden well) were 52.5±6.5 in control group, 93.5±10.1 in 50 mg protein/L oxidized high density lipoprotein group(p<0.05), 148.5±21.6 in 100 mg protein/L oxidized high density lipoprotein group(p<0.01), 112.8±19.9 in oxidized high density lipoprotein+E 2 (17 β estradiol) group(p<0.05, compared with 100 mg protein/L oxidized high density lipoprotein group), and without significant changes in HDL groups. MCP 1 in the conditioned medium of THP 1 cells were as follows: 76.5±11.6 ng/g protein in control group, 141.0±19.5 ng/g protein in 100 mg protein/L oxidized high density lipoprotein group(p<0.01), 117.5±15.2 ng/g protein in oxidized high density lipoprotein+E 2 group(p<0.05 compared with oxidized high density lipoprotein group), and without significant change in HDL group. Conclusions oxidized high density lipoprotein could stimulate the migration of THP 1 cells and the production of MCP 1. It suggested that oxidized high density lipoprotein may participate in the primary pathogenesis of atherosclerosis by promoting the migration of monocyte to the intima of artery and increasing MCP 1; 17 β estradiol may have protective effect.

Abstract:Aim The pathological mechanism of high occurrence of atherosclerosis in diabetes mellitus (DM) has not been clear. The purpose of this study is to detail its possible mechanism by studying whether high glucose and LDL could synergetically injure vascular endothelial cells (ECV304). Methods Glucose (5.5, 20 and 40 mmol/L) and/or LDL (100 mg/L) was added into the medium of cultured endothelial cells and incubated for 24, 48, and 72 h respectively. The lipid peroxidation was assessed by the content of TBARS, apoptosis and cell cycle were studied with Giemsa staining, transmission electron microscopy and Coulterepics SP. Results (1)High glucose could enhance cell mediated LDL peroxidation and showed a position of dose effect relationship. (2)High glucose and LDL had synergetical effects on inducing apoptosis. This may be preferentially related to the cell mediated LDL peroxidation enhanced by high glucose and prolongation cell cycle traversal of endothelial cells through oxidative stress produced by high glucose. Conclusion High glucose and LDL could synergetically induce apoptosis in endothelial cells, which aggravate the pathogenesis of atherosclerosis in diabetes mellitus. This may at least partly attribute to the mechanism that diabetes mellitus mostly accompany high risk of atherosclerosis.

Abstract:Aim To investigate the relationship between the promoter region of Bβ fibrinogen (Fg) gene polymorphism 148C/T, 455G/A and coronary heart disease (CHD). Methods By Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) restriction enzyme digestion methods,the genotypes and allele frequencies of Bβ fibrinogen gene polymorphism 148C/T, 455G/A were determined in 148 CHD patients and 173 controls. Result Fg Bβ 148T, 455A allele frequencies are higher in CHD group than that in control group (p<0.05). Both heterozygous genotype frequencies of 148CT and 455GA are higher in CHD group than that in control group. The plasma Fg levels have differences among genotypes: 148CC, 455GG< 148CT, 455GA< 148TT, 455AA respectively. The plasma Fg levels are higher in CHD group than that in control group, when compared the same genotype, CHD group Fg levels also higher than that of in control group. Conclusion The promoter region of Bβ Fg gene polymorphism 148C/T, 455G/A are associated with the development of CHD, and at same time impact on the plasma Fg concentration. The polymorphism 148C/T and 455G/A are complete linkage disequilibrium in Southern Chinese.

Abstract:Aim To explore the effects of oral fatty loads with different ratios of saturated, monounsaturated and polyunsaturated fatty acids on the metabolism of postprandial triacylglycerol in triacylglycerol rich lipoproteins (TGRL) and to find a suitable fatty acid composition ratio used in the oral fatty load. Methods The oral fatty loads with three different fatty acids ratios (1∶1∶1, 1∶1.7∶1.2 and 1∶1.7∶2.3) were carried out in 28 type 2 diabetic patients. There were 9,9,10 patients in the group 1∶1∶1, 1∶1.7∶1.2 and 1∶1.7∶2.3, respectively. All the patients were matched for sex, body mass index and age. Plasma were obtained before and 2, 4, 6 h after the fatty load. TGRL were subfractioned to Sf～400, Sf60～400, Sf 20～60 and Sf12～20 fractions by cumulative floatation in a density gradient from plasma. The whole plasma triacylglycerol (TG) and TGRL subfractions triacylglycerol were determined. Results The postprandial TG peak value of the total plasma TG and TG in four subfractions of TGRL as well as the total TG, Sf>400 TG and Sf 60～400 TG average concentration in all groups were significantly higher than fasting TG, respectively (p<0.05). The total plasma TG in group 1∶1∶1 peaked at 2～4 h after the fatty loads, in group 1∶1.7∶1.2 at 4 h but in group 1∶1.7∶2.3 at 4～6 h. Sf>400 TG peaked at 2～4 h after the fatty loads in both group 1∶1∶1 and group 1∶1.7∶1.2 but 2～6 h in group 1∶1.7∶2.3. Increment of total plasma TG at 2 h was significantly higher in group 1∶1.7∶1.2 than in group 1∶1.7∶2.3. (p<0.05), increment of postprandial average concentration of triacylglycerol in Sf12～20 TGRL was significantly lower in group 1∶1.7∶1.2 than in group 1∶1∶1 and group 1∶1.7∶2.3 (p<0.05). MUFA/PUFA was the important factor influencing the increment of the whole plasma triacylglycerol at 2 h after the fatty load. MUFA/SFA was the important factor influencing the increment of postprandial average concentration of the whole plasma triacylgleycerol. The whole plasma triacylglycerol was determined by Sf>400 triacylglycerol rich lipoproteins triacylglycerol. Conclusions The data showed that the fatty meals with different fatty acids composition ratios led to the different patterns of the whole plasma triacylglycerol and to that of TGRL triacylglycerol. An oral fatty load with a dietary fatty acid composition ratio of 1∶1.7∶1.2 could be used in postprandial lipemia and TGRL study.

Abstract:Aim To examine carotid intima media thickness (IMT) and its determinants in elderly type 2 diabetes(DM)subjects, classified according to the new criteria of WHO 1999, in comparison with age and sex matched controled subjects with normal glucose level. Methods We compared the results of common carotid IMT and plaques from the subjects of type 2 diabetics aged 60～84 years with that of the non diabetic as controls. Results The diabetes subjects, both men and women, displayed carotid intima media thickness (p<0.05). Among the coronary risk factors, triglycerides (TG) was significantly increased and high density lipoprotein cholesterol (HDLC) decreased. In the diabetic group, IMT was correlated to triglycerides, TC/HDLC(p<0.05), HbA1c (p<0.05), blood pressure, age, sex, and duration. Conclusions The results suggest that hyperglycemia, together with a clustering of risk factors, and in particular dyslipidemia, may cause intima media thickness in the elderly type 2 diabetic patients.

Abstract:Aim To study the inflammatory reaction in the arteriosclerotic lesions and effect of it by interventions of lipid lowering in the patients of Acute cerebral infarction(ACI)with hyperlipidemia. Methods To measure the level of soluble plasma P selectin and oxidized low density lipoprotein (ox LDL) by ELISA and serum nitric oxide (NO) by colorimetric analysis method before and after treatment with simvastatin in 30 patients of ACI with hypercholesterolemia(HC group), 28 patients of ACI with hypertriglyceridemia(HTG group) and 36 normal controls. Results The patients with ACI of HC group and HTG group had higher levels of plasma P selectin and ox LDL, lower level of serum NO than control group. A direct positive correlation was observed in P selectin level with ox LDL, LDLC and ox LDL, TG in HC group and HTG group separately. A direct negative correlation was found between plasma P selectin and NO levels in patients of HC group. After administration of simvastatin (20 mg/d for 6 weeks) to patients of HC group and HTG group, plasma P selectin and ox LDL levels reduced significantly and serum NO level elevated significantly. Conclusions The inflammatory reaction in the arteriosclerotic lesions exists both in the patients of ACI with hypercholesterolemia and hypertriglyceridemia, the development of it is synchronized with the levels of plasma P selectin, ox LDL,TG and serum NO, which can be blocked by intervention of lipid lowering.

Abstract:Aim To evaluate the clinical and coronary angiographic features in elderly patients with type 2 diabetes mellitus combined with coronary heart disease. Methods A total of 133 cases confirmed by coronary angiograph were divided into two groups according to the status if they had combined with type 2 diabetes mellitus or not. Then the study was carried out by case control analysis of the clinical and the data from angiograph. Results High triglyceride, low high density lipoprotein cholesterol and low left ventricular election fraction levels were found in the group of type 2 diabetes mellitus combined with coronary heart disease. Also in this group, the lesions were mainly found in left main coronary artery and all three vessels. The abnormalities of wall motion was more common in this group and the collateral circulation was more abundant than group without typE₂ diabetes mellitus. Through Logistic regression analysis, advanced age, high triglyceride and low high density lipoprotein cholesterol levels were confirmed to be independent risk factors. Conclusions In elderly patients with type 2 diabetes mellitus combined with coronary heart disease, the lesions were mainly found in left main coronary artery and all three vessels. Advanced age, high triglyceride and low high density lipoprotein cholesterol levels played an important role in the severity of coronary artery lesions in this kind of patients.

Abstract:Aim To investigate the sCD40L levels in acute coronary syndrome and stable coronary heart disease and its relationship with serum lipids. Methods Enzyme linked Immunosorbent Assay (ELIA) was used to measure the serum soluble CD40 ligand (sCD40L). Results sCD40L level was significantly higher in patients with acute coronary syndrome (3.17±2.84 μg/L) than in control (1.19±1.05 μg/L, p<0.01) and in stable coronary heart disease patients (1.61±1.46 μg/L, p<0.05). Soluble CD40L level was significantly related to TG (r=0.23, p<0.05), apoB (r=0.248, p<0.05), and HDLC (r=-0.253, p<0.05). Conclusions This study showed that sCD40L level was elevated in ACS, suggesting the possible relation of CD40L to the pathogenesis of ACS and it may save as a potential marker of plaque stability.

Abstract:Aim To investigate the effect of hypertension and insulin resistance on mononuclear cell binding to cultured endothelium. Methods Insulin sensitivity was estimated by modified insulin suppression test and endothelial adhesiveness for mononuclear cell was assessed by functional binding assay in 33 hypertensive patients and 32 normal controls. Results Mononuclear cell binding to endothelium increased in patients with hypertension compared to normal controls (35±9 vs 28±10,p<0.01). There was a positive relationship between mononuclear cell binding and mean arterial pressure (r=0.43,p<0.001). A positive relationship also existed between steady state plasma glucose (SSPG) concentration and mononuclear cell binding in both the hypertensive (r=0.61,p<0.001)and normotensive (r=0.71,p<0.001)groups. Multiple regression analysis demonstrated an independent relationship between mononuclear cell binding and both SSPG (p<0.001) and insulin (p<0.01). Conclusions Hypertension and insulin resistance enhance mononuclear cell adherence to cultured endothelium. It may be the linkage between atherosclerosis and both hypertension and insulin resistance.

Abstract:Aim To detect the early diagnosis value of thrombus precursor protein (TpP) in acute coronary artery syndrome. Methods 51 cases of coronary artery disease were divided into two subgroups of unstable pectoris angina and acute myocardial infarction. Thrombus precursor protein and creatine kinase (CK and CKMB )concentration were determined in all patients. Resluts Thrombus precursor protein concentration of the acute myocardial infarction subgroup increased significantly than the unstable pectoris angina subgroup(9.9±3.9 mg/L vs 2.6±1.8 mg/L,p<0.01);also,CK and CKMB concentration of the acute myocardial infarction subgroup increased significantly than the unstable pectoris angina subgroup (595±432 u/L vs 137±40 u/L,p<0.05; 101±74 u/L vs 10±7 u/L,p<0.01,respectively). Conclusion Thrombus precursor protein concentration of the acute myocardial infarction subgroup increases significantly,but not in the unstable pectoris angina subgroup. Thrombus precursor protein has differentiated and early diagnostic value for the acute myocardial infarction in patients with acute coronary artery syndrome.

Abstract:Aim To study the hemodynamic effects of fructose 1,6 diphosphate (FDP) in patients with coronary artery disease (CAD) complicated with chronic heart failure (CHF). Methods 10 g FDP was intravenously infused in 47 cases of CAD with CHF. Heart rate (HR), mean arterial pressure (MAP), ejection fraction (EF), stroke volume (SV), stroke index (SI), cardiac output (CO), cardiac index (CI) and fractional shortening (FS) were measured before, 30 min and 60 min after the infusions. Results The results indicated that apart from EF 30 min after versus before the infusions, all of the cardiac function parameters dramatically increased after the treatment while the augmentations were larger in 120 min than in 30 min after the treatment. Conclusions FDP intravenous administration in patients with CAD complicated with CHF could improve the hemodynamic status and cardiac function. The drug possessed positive inotropic property but would not increase O 2 consumption.