Abstract:Aim Elevated plasma homocysteine impairs vasomotor regulation. Hyperhomocysteinemia (HH) is an independent risk factor for atherosclerosis, which causes peripheral arterial occlusive disease. Thus, we examined whether HH induced by oral methionine load modulated in vivo angiogenesis in a rat model of hindlimb ischemia. Methods Male Sprague-Dawley rats were divided into 2 groups: the control group was given tap water, and the other group was made HH by daily intake of L-methionine (1 g/kg body weight) in drinking water. At the 14th day of the dietary modification, the left femoral artery and vein were excised, and the extents of angiogenesis and collateral vessel formation in the ischemic hindlimb were examined for subsequent 4 weeks. Results Plasma homocysteine levels significantly increased, and plasma levels of nitric oxide and tissue contents of nitric oxide and cyclic guanosine 3, 5-monophosphate significantly decreased in the HH group. Serial laser Doppler blood perfusion analysis revealed significant decrease in the ratio of ischemic/normal hindlimb blood perfusion at postoperative days 7,14,21 and 28. Postmortem angiography of the ischemic limb revealed a significant decrease in the angiographic score at postoperative day 14 in the HH group. Finally, immunohistochemical examination of the ischemic tissue sections showed a significant decrease in capillary density in the HH group. Conclusions In summary, oral methionine-induced HH impairs ischemia-induced angiogenesis and collateral vessel formation in a rat model of chronic hindlimb ischemia. The mechanism appears to be mediated at least in part by decreased bioavailability of endothelium-derived nitric oxide (EDNO) in the HH state.

Abstract:Aim To investigate difference expression of peroxisome proliferator-activated receptor α (PPAR α) during aging and explore the molecular mechanism of perturbation in lipid metabolism during aging. Methods We used two-group SD rats to estimate the lipid level of young (6～8 weeks) and aged (24 months) groups. The levels of triglyceride (TG) and total cholesterol (TC) were examined. The levels of liver PPAR α and target genes mRNA were measured by reverse transcription-polymerse chain reaction (RT-PCR) and PPAR α protein were determined by western blotting respectively. Results The TG and TC level of the aged group increased significantly. The PPAR α mRNA and protein level of the aged group significantly decreased compared with the young group. Target gene levels were changed during aging. Conclusion The mechanism of lipid dysfunction during aging is probably associated with the decreased expression of PPAR α.

Abstract:Aim To investigate the mechanism of the change in the reactivity of the remodeling vessels of hypertension to Angiotensin Ⅱ(AngⅡ). Methods Use the method of Video-Microscope to observe the contractility of the third embranchment of the mesentery artery of the normotensive Sprague-Dawley (SD) rats and the two-kideny one-clip (2K1C) renal hypertension(RH) rats to AngⅡ. Use the method of isolation vascular ring perfusion to exploration the activity to AngⅡ of the aortic rings either group rats and the effect of losartan on the actibity. Use RT-PCR to measure AT1R mRNA and AT2R mRNA expression in the thoracic aorta of either group rats. Results All of the contractility of the aortic rings and the third embranchment of the mesentery artery to AngⅡ increased with dose-dependented, and the constractility of the remodeling vessels of the 2K1C RH rats to AngⅡ toned up more evidently at every dose of angiotensionⅡ. Loarstan block the the contractility of the aortic rings to AngⅡ completely. AT1R mRNA expression in the thoracic aorta of 2K1C RH rats is more than which in the the thoracic aorta of normotensive rats and AT2R mRNA express only in the thoracic aorta of 2K1C RH rats. Conclusions (1) The response of the remodeling vessel in hypertension to AngⅡtone up . (2) The mechanism of change in the reactivity of remodeling vessel of renal hypertension rats to Angiotensin Ⅱ may due to AT1R mRNA up-regulated.

Abstract:Aim To observe the effects of Sini decoction on atherosclerosis and oxidative injury in rabbits fed with cholesterol rich diet. Methods 24 rabbits were randomly divided into three groups. Normal group was fed with normal diet; Model group was fed 1% cholesterol and 5% fat diet; Sini decoction group was fed 1% cholesterol and 5% fat diet plus Sini decoction [2.1 g/(kg·d)]. At the end of study, the plaque area were measured, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of blood and aorta were detected. SOD protein and gene expression were observed with immunohistochemistry and hybridization in situ method. Results Compared with model group, Sini decoction could diminish lipid plaque area, intimal plaque area and intimal thickness, reduce the levels of aorta and serum MDA, increase aorta and serum SOD activity, increase the expressions of SOD protein and SOD gene in aorta. Conclusions The experimental results suggested that Sini decoction had antiatherogenic effects. The possible mechanism might be that Sini decoction could reduce oxidative injury in rabbits.

Abstract:Aim To study the effect of apolipoprotein CⅡ promoter T→A mutation at position -190 on the transcription of the apolipoprotein CⅡ gene and its relationship to the decrease of apolipoprotein CⅡ concentration in plasma. Methods Firstly, the expression vector pGL3 with -190 T→A mutant promoter was constructed using site-directed mutagenesis; Secondly, the expression vector pGL3 with mutant promoter and normal promoter respectively was co-transfected with pRL-TK as an internal control into HepG2 cells by liposome-mediated methods, and expressed transiently. Thirdly, Both firefly luciferase activity drived by normal/mutant apolipoprotein CⅡ promoter from pGL3 and renilla luciferase activity drived by TK promoter from pRL-TK were measured by dual luciferase reporter assay system. Results The results showed that the T→A mutation at position -190 in apolipoprotein CⅡ promoter caused a decrease of 18.96% in luciferase activity compared with the normal apolipoprotein CⅡ promoter. Conclusions The T→A mutation of -190 base in apolipoprotein CⅡ promoter region could debase transcription activity of apolipoprotein CⅡ promoter.

Abstract:Aim To investigate the effect of angiotensin converting enzyme (ACE) antisense cDNA on the synthesis of collagen of the cardiac fibroblasts (Fbs) from spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY). Methods In vitro, Fbs from SHR and WKY rats was transferred with target ACE antisense cDNA (AS),ACE tuncated cDNA (TS) and empty vector (control) mediated by liposome. Two days after transferation, the target gene expression and the effect on ACE expression was evaluated with reverse transcription polymerase chain reaction, and the target ACE activity of the cells was tested with test reagent. The collagen synthsis of Fbs was tested with 3 H-proline incorporation. Results The expression of ACE antisense cDNA could be detected in Fbs,and the expression peak was in the fourth day after transfection, and it was concomitant with the decrease of ACE expression. This was associated with a significant decrease in ACE activity (14.23±1.62 vs 26.53±2.50 u/10 5 cells,p<0.05) level in target cells and culture medium. However, there was not same effect on Fbs from WKY. AS group had significantly lower 3 H-proline incorporation rate in Fbs from SHR than TS and control group (4 355.7±75.9 vs 6 310.3 ±98.9, 6 529.5±192.5 cpm/10 5 cells, p<0.01). Conclusions ACE antisense cDNA can inhibit the higher basal collagen synthesis in Fbs. Present finding suggests that ACE antisense cDNA will be useful and possible in the gene therapy of higher collagen synthesis related disease and cardiovascular remodeling.

Abstract:Aim To clarify the relation of expression change of tumor necrosis factor-α (TNF-α) and angiotensin Ⅱ (AngⅡ)in hypertrophic mayocardial on stress load. Methods Sampling blood and extirpate heart after 42 days in the cardiac hypertrophy model of exceed stress load were made by abdomen aorta-constriction; determining hypertrophic exponent and the concentration of AngⅡin the plasma and myocardium by Ratio-immunity; determining TNF-α in serum and left ventricle by enzyme-linked immunoadsordent assay. Results Left ventricle showed obvious hypertrophy after operation 42 days; the concentration of TNF-α and AngⅡ in myocardium heighten 6 folds and 1 fold than controls (p<0.01); Being treated by Captopril can restrain the progress of left ventricle hypertrophy after operation and descend the content of TNF-α in myocardium 64.14% (p<0.01), but can not descend to the level of sham-operated group (p<0.01). Being the content of AngⅡ descend 45.73% (p<0.01)and had no marked significance to the level of sham-operated group. Conclusions The activation of cardiac renin-angiotensin system (RAS) by excess stress load result to heighten TNF-α in myocardium and it is probably one of the major adjusting path of myocardial hypertrophy.

Abstract:Aim To investigate the changes of sugar and lipid metabolism in syndrome X rats and examine the intervenient effects of rosiglitazone on them. Methods Syndrome X was produced by two-kindey, one-cliped (2K1C) rats fed with high fructose. Systolic blood pressure (SBP), blood lipid, the level of blood sugar and serum insulin were measured respectively in groups, and insulin sensitive index (ISI) was also calculated. Results At 8 weeks, compared with sham operation group, SBP, triglyceride (TG), blood total cholesterol (TC), low density lipoprotein cholesterol (LDLC), fasting blood sugar (FBS), fasting serum insulin (FSI) significantly increased; insulin sensitive index (ISI), high density lipoprotein cholesterol (HDLC) significantly reduced in groups with or without rosiglitazone treatment. At 11 weeks, in the group with rosiglitazone treatment, TG, FBS and FSI remarkably reduced, TC, HDLC and ISI significantly elevated, but LDLC only slightly increased; While other two groups did not change significantly compared with that at 8 weeks. Conclusions Rosiglitazone can reduce BP, improve insulin resistance and correct the abnormity of sugar and lipid metabolism.

Abstract:Aim To investigate the effects of platelet-derived growth factor-BB (PDGF-BB)on content of calmodulin in human vascular smooth muscle cells. Methods The human vascular smooth muscle cells were cultured and effects of PDGF-BB on content of calmodulin in human vascular smooth muscle cells were assayed at different time and different concentration by its ability to stimulating calmodulin dependent cyclic nucleotide phsphodiesterase in vitro. Results Calmodulin level of quiescent human vascular smooth muscle cells was increased in its cell cycles with a maximal response 9 h, and PDGF-BB could significantly increase content of calmodulin of human vascular smooth muscle cells in a dose-dependent manner, with a maximal response at a concentration of 30 μg/L. Conclusion PDGF-BB could significantly increase calmodulin level of quiescent human vascular smooth muscle cells in a dose-dependent manner.

Abstract:Aim To evaluate the effect of silybin on neointimal proliferation of rabbits iliac artery after balloon injury. Methods 22 rabbits were randomly separated into 3 groups including control group, low-dose treatment group and high-dose treatment group. Balloon catheter was used to induce injury to the iliac artery of rabbits in treatment group. Tranilast was given to the rabbits in low-dose treatment group [20 mg/(kg·d)] and high-dose treatment group [40 mg/(kg·d)] once a day from 3 days before operation to 28 days after operation, and then local vessel was harvested for pathological analysis and immunohistochemical staining. Automatic image analysis system was used to detect the changes of intimal thickness, medical thickness, luminal area, and the proliferation index of proliferating cell nuclear antigen (PCNA). Results No significant difference was found between low-dose treatment group and control group in intimal thickness, medial thickness, luminal area and proliferation index of PCNA (p> 0.05 ). The intimal thickness was decreased in high-dose treatment group compared with control group (p< 0.05 ). The luminal area was significantly larger in high-dose treatment group than that in control group (p<0.05). The proliferation index of PCNA was significantly reduced in high-dose treatment group compared with control group (p<0.05). Conclusions Silybin significantly inhibit neointimal hyperplasia and the drug may be used to prevent restenosis.

Abstract:Aim To study the possible mechanisms of folic acid on hyperhomocysteinemia. Methods Rabbit aorta endothelial cell (EC) were isolated and cultured, then divided into six groups after adding native rabbit low density lipoprotein (nLDL) into the EC medium: control group (without any more agents added), folic acid group, tetrahydrobiopterin (BH 4)group, homocysteine (Hcy)group, folic acid plus Hcy group and BH 4 plus Hcy group. The following items were determined that is ① the degree of lipid peroxidation, ② the amounts of NO 2ˉ/NO 3ˉ, and ③ the activity of NO synthase (NOS). Results Folic acid plus Hcy group were similar with BH4 plus Hcy group. The various effects of Hcy were antagonized in both groups.Compare with Hcy group, the degree of lipid peroxidation significantly decreased (p<0.01), the amounts of NO significantly increased (p<0.01). The activity of NOS also increased (++). Conclusion There were same effects both folic acid and BH4. They may inhibit the co-injure of Hcy and LDL on EC by protecting effects of NO system.

Abstract:Aim To investigate the effects of cerivastatin on expression of matrix metalloproteinase (MMP-9) and CD40 in THP-1 cells. Methods THP-1 cells were incubated with increasing concentration of cerivastatin. MMP-9 and CD40 mRNA were detected by RT-PCR, MMP-9 concentration in media were assayed by ELISA. Results Cerivastatin inhibited the expression of CD40 and MMP-9 concentration dependently. At 0.01 μmol/L, the mRNA levels of CD40 and MMP-9 were not significantly decreased. But expression of CD40 and MMP-9 mRNA were significant down-regulated at 1 to 10 μmol/L; Level of MMP-9 concentration in media decreased from 0.469±0.06 to 0.243±0.04 and 0.039±0.01 respectively at 1 or 10 μmol/L(p<0.05). Conclusions Cerivastatin inhibited the expression of CD40 and MMP-9 in THP-1 cells, decrease the MMP-9 concentration in media, it suggest that HMG-CoA reductase inhibitors may suppress inflammatory signaling and plaque disruption which responsible for clinical acute coronary syndrome.

Abstract:Aim To study the relationship between human cytomegalovirus (HCMV) infection and atherogenesis and the putative mechanism. Methods The HCMV IgG and C-reactive protein (CRP) of subject serum and the existence of HCMV immediate early (IE) gene in the atherosclerotic plaque were detected by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The expression of chemokines in endothelial cells after HCMV infection was also studied. Results The positive ratio of HCMV IgG was significantly higher in atherosclerosis group than that in non-atherosclerosis group (82.2% vs 61.0%, P=0.02); the serum CRP level of atherosclerosis group was significantly higher than that of non-atherosclerosis (5.912±3.795 mg/L vs 2.871±1.761 mg/L, P=0.000); HCMV-specific gene in atherosclerotic plaque was much more frequently detected than that in normal vascular tissue (13/15 vs 2/7, P=0.01). HCMV infection could upregulate the expression of chemokines monocyte chemoattractant protein-1 and fractalkine in endothelial cell ECV-304. Conclusions HCMV infection is involved in the pathogenesis of atherosclerosis, which may be mediated by up-regulated expression of chemokines in ECV-304 after HCMV infection.

Abstract:Aim To investigate the relations between Helicobacter pylori(Hp) infection and acute myocardial infarction. Methods The concentration of Hp specific IgG antibody and the optical density (OD) of Hp specific IgM antibody were measured in 51 patients with acute myocardial infarction (AMI group), 42 patients with old myocardial infarction (OMI group) and 31 normal controls (NC group) who had angiographically confirmed no coronary artery disease. Simultaneously, the changes and relationships between C-reative protein (CRP), fibrinogen (Fg) and Hp infection were determined in the three groups. Results 39 of the AMI group (76.47%) were seropositive for Hp average concentration (OD) of IgG (IgM) antibody to Hp was significantly higher in the AMI group than that of the NC group (p<0.05), but they were not significantly higher than that of the OMI group (p<0.05). The relation between the Hp infection and acute myocardial infarction (AMI) was strong (odd ratio is 3.047, 95%CI is from 1.055 to 8.796, χ 2=24.241, P=0.03). Adjustment for the major cardiovascular risk factors didn't attenuate this relation (odds ratio is 3.262, 95%CI is from 1.015 to 10.485, χ 2=23.94, P=0.047). The average concentration of IgG antibody to Hp, the levels of Fg and CRP were significantly higher in the AMI group compared with those in the NC group. Those who were Hp positive in the AMI group had not only significantly hiher levels of CRP and Fg than those who were Hp negative in the same group (p<0.05) but also than those who were Hp positive in the OMI group (p<0.001). There was still a positive relation with the average contentration of IgG to Hp in Hp positive patients of the AMI group (p<0.05). Conclusion There is a significant relation between Hp infection and acute myocardial infarction. There is a certain relation between Fg, CRP and Hp infection, too.

Abstract:Aim To investigate the intervenient efficacy of lipid lowing therapy as well as blood pressure controling in carotid arteriosclerosis and stroke of patients with hypertension. Methods The carotid artery colordopler ultrasonography was examed and the levels of serum lipids were measured in 102 cases of hypertension using the Micronised Ferofibrate compared with 105 controls during 24 monthes. Results The intima-media thickness (IMT) of carotid artery was almost unchanged in lipid lowing intervention group after this period of treatment. The IMT/D ratio and the incidence of carotid arteriosclerosis plaque were reduced signifficantly after the therapy.The relative risk of stroke in intervention group was lowered by 34% compared with the controls. Conclusion The carotid arteriosclerosis and the stroke in hypertension could be well prevented with lipid lowing therapy while the patient's blood pressure had been controled.

Abstract:Aim To investigate the association of cholesteryl ester transfer protein (CETP) and CETP TaqⅠB polymorphism with type 2 diabetic macroangiopathy in Chinese. Methods The level of serum CETP was measured by enzyme-linked immunosorbent assay (ELISA) and CETP genotype was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 96 healthy controls and 226 patients with type 2 diabetes mellitus. Results ①The serum level of CETP in type 2 diabetes groups was significantly higher than that in controls, and its level in patients with macroangiopathy was higher than that in patients without macroangiopathy. ②The frequency of genotype B1B1 (37.3%) in type 2 diabetes with macroangiopathy significantly higher than that in patients without macroangiopathy (16.4%). Compared with genotype B₂B₂ ( 29.1% ) class, the morbidity of macroangiopathy in genotype B1B1 (68.3%) or B1B₂ (46.6%) class was significantly increased. ③Multiple logistic analysis showed that the serum CETP and its B1 allele were the important risk factors of type 2 diabetic macroangiopathy. Conclusions ①Serum CETP level rises in diabetic patients and high CETP level may be an important risk factor for type 2 diabetic macroangiopathy. ②CETP TaqⅠB polymorphism is associated with the development and progression of type 2 diabetic macroangiopathy. B1 allele may be a susceptible gene of type 2 diabetic macroangiopathy.

Abstract:Aim To explore the role of inflammation in acute coronary syndrome (ACS) and the mechanism by which statin treat ACS. Methods The serum high-sensitivity C-reactive protein (hs-CRP) and interleukin-6 (IL-6) levels were measured in 50 patients with ACS, including 30 patients with unstable angina (UA) and 20 patients with acute myocardial infarction (AMI), 34 patients with stable angina (SA) and 30 controls. 30 patients in ACS group were randomly selected and assigned to the simvastatin group (n=15) and the routine group (n=15). The simvastatin group was given simvastatin 20 mg/d and the routine group took placebo expect routine treatment (including nitrate, aspirin , β-blocker and ACEI ). After a three-week follow-up, serum hs-CRP, IL-6 levels and serum lipids concentrations were measured again. Results Both serum IL-6 and hs-CRP levels were significantly higher in ACS group than in SA and normal control group(p<0.001). After three-week treatment with simvastatin, serum IL-6, hs-CRP, total cholesterol (TC) and low density lipoprotein cholesterol (LDLC) levels decreased significantly in the simvastatin group (p<0.001), but not in the routine group. The rates of decrease of IL-6 and hs-CRP was significantly different between simvastatin group and routine group (p<0.05). No relationship was observed between the values of decrease of serum IL-6 or hs-CRP and serum lipids levels. The serum hs-CRP level showed significant correlation with coronary heart disease (CHD) in both univariate regression pattern and multivariate regression pattern (p<0.05) . Conclusions Inflammation plays an important role in the initiation of ACS. Assaying serum IL-6 and hs-CRP levels may be able to help to definite the diagnosis of ACS. Simvastatin possess anti-inflammatory effect which independent of its lipid lowering action, and the anti-inflammatory effect of simvastatin might play an important role in the early treatment of ACS.

Abstract:Aim To study the association of interleukin-6 (IL-6) gene -572C/G polymorphism with coronary heart disease (CHD). Methods IL-6/-572C/G polymorphism was examined in 139 CHD patients and 168 healthy adults by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results -572CC, CG and GG genotype frequencies were 51.80%, 40.29% and 7.91% in CHD group, 63.10%, 35.71% and 1.19% in control group respectively. -572 G and C allele frequencies were 28.06%, 71.94% and 19.05%, 81.95% in two groups respectively. GG genotype and G allele were more frequent in patients than in controls (p<0.01). Compared with CC genotype, relative risk (RR) for CHD were 1.37 (95%CI: 0.86～2.20, p>0.05) for CG genotype and 2.85 (95%CI: 1.32～6.13, p<0.01) for GG genotype. After adjusting for classical risk factors, the effect of GG genotype remained statistically significant (Rr=2.85, 95%CI: 1.22～6.65,p<0.05). But the distribution of IL-6/-572C/G polymorphism had no significant difference among patients with stable angina, unstable angina and myocardial infarction, among patients with single-vessel,two-vessel and there-vessel lesions too(p>0.05). Conclusions IL-6/-572C/G polymorphism was associated with susceptibility to CHD and GG genotypes might be a risk factor for CHD in Chinese Hans population.

Abstract:Aim To demonstrate the relationship between stenosis degree as well as plaque morphology and cerebral ischemia. Methods The inner carotid arteries of 10 patients with cerebral ischemia before death were removed en block in autopsy. After fixation, the arteries were serially cut into blocks in an interval of 4 mm and HE sections were made from every block. The HE sections were microscopically observed and the lumen area and lipid core area were measured through computer image analysis system. 10 control cases without cerebral ischemia before death were also studied, the study method and measured parameters were similar to the cerebral ischemia groups. Results Among the total 151 blocks in cerebral ischemia group, the lumen area was 6.2 mm 2 in maximum and 3.2 mm 2 in minimum, the average was 4.7±0.3 mm 2. In control group, the maximum and mimimum lumen area was 6.5 and 3.6 mm 2, with an average of 4.8±0.4 mm 2. There was no significant difference between the two groups. While the average lipid core area in cerebral ischemia group and control group was 3.4±0.4 and 1.8±0.2 mm 2 respectively. The difference was significant (p<0.01). In addition, 11 thromboli were found in the cerebral ischemia group, but no thrombus was found in the control group. Conclusion there was no significant difference of stenosis between cerebral ischemia and control groups, but the lipid core area in cerebral ischemia group was larger than that of control group. The stenosis degree may not take a more great role in the occurrence of cerebral ischemia than the lipid core size does.

Abstract:Aim To study the association between the ratio of fasting blood triglyceride to high density lipoprotein cholesterol (TG/HDLC) and macrovascular complications in aged type 2 diabetes in Guangxi. Methods To investigate some marks such as fasting blood glucose (FBG), posty prandial blood glucose (PBG), fasting TG, HDLC, low density lipoprotein cholesterol (LDLC), TC, blood pressure (BP) and body mass index (BMI) of 551 above-60-aged type 2 diabetes in the survey of chronic complications of The Diabetes Society of Chinese Medical Association. Results PBG, SBP, TC and BMI of high TG/HDLC ratio group (R>1.33) is significantly higher than that of normal TG/HDLC ratio group (p<0.05); So does the prevalence rate of macrovascular complications (p<0.05); single-factor linear correlation analysis show that FBG, PBG, TC, LDLC, duration of DM and BMI, etc have positive correlation with TG/HDLC ratio (p<0.05); Multiple factor stepwise regression analysis show that the main factors affecting TG/HDLC ratio are FBG, TC, LDLC, BMI and duration of DM (p<0.05). Conclusion Dyslipidosis has association with the prevalence rate of macrovascular complications in aged type 2 diabetes. TG/HDLC ratio can indicate reliably the occurrence of macrovascular complications.

Abstract:Aim To explore the correlation between incidence of coronary heart disease (CHD) and the changes of markers of coagulation, fibrinolysis in patients with CHD. This may help to diagnosis, anticoagulation, thrombolysis and prognosis for patients with CHD. Methods Plasma platelet granule membrane protein 140 (GMP-140), von Willebrand factor (vWF), D-dimer (D-D) were detected by enzyme linked immunosorbent assay (ELISA) in 100 patients with CHD, and compared with 42 healthy subjects. Results Plasma GMP-140, vWF, D-D were significantly elevated in patients with CHD and differed significantly compared with control group (p<0.01). The changes of molecular markers in patients with acute myocardial infarction (AMI) were elevated significantly than those with old myocardial infarction (OMI), unstable angina (UA) and stable angina (SA) (p<0.01). The levels of plasma GMP-140 in patients with UA were elevated more significantly than those with SA (p<0.05). There were obvious correlations between the molecular markers in patients with CHD(r=0.857～0.301, p<0.01). Conclusions An obvious hypercoagulable state existed in patients with CHD. There were correlations between the changes of molecular markers of hypercoagulable state and patients, condition.

Abstract:Aim To screen the lipoprotein lipase gene for mutations in Chinese population and to study the possible effects of the mutations on the lipoprotein metabolism. Methods The lipoprotein lipase gene was examined by polymerase chain reaction-single strand conformation polymorphism analysis, and the polymerase chain reaction products showing abnormal pattern on single strand conformation polymorphism were sequenced using dideoxy-mediated chain-termination method. Results Two mutations were found in 140 Chinese people and both were heterozygous C→T transitions at 6 bp upstream from acceptor splicing site of intron 3. Conclusion The mutation in intron 3 of the lipoprotein lipase gene may be the genetic risk of hyperglyceridemia in Chinese population.

Abstract:Aim To study lipoprotein lipase (LPL) gene espression in vitro and observe the mouse LPL activity in post-heparin plasma and various tissue samples we adopted a standard method using radioactive emultion as substrate. Methods 3H-labeled triolein emulsion was prepared and used for subsequent incubation with different enzyme sources. Results There was high LPL activity expression in cultured 293A cells infected with recombinant adenovirus encoding human LPL. The LPL activity of post-heparin plasma,cardiac muscle,and adipose tissue of mice were 314.3 U/L, 349 mU/g and 61 mU/g respectively. Conclusions The method of determination for LPL activity was sensitive,accurate and stable. The results of LPL activity of post-heparin plasma and various tissue samples of mice provided helpful data for further research.

Abstract:Aim To examine the effect of implantation of intracoronary stents on the serum levels of C-creative protein (CRP). Methods This study included 105 patients who underwent implantation of intracoronary stents and 242 patients who received coronary angiography (CAG). Serum CRP was measured in the early morning before the procedure and the following morning after the procedure. Results In patients with implantation of stents, CRP levels after the procedure were significantly higher than that before procedure (6.7±0.5 vs 17.6±1.0, p<0.01). In CAG group, however, CRP levels after angiography were not significantly higher than that before angiography (3.5±0.7 vs 3.6±0.8, p>0.05). Conclusions Implantation of intracoronary stents leads to a detectable increase in serum levels of CRP, which is probably related with plaque rupture, the intrinsic inflammatory or tissue damage within arterial intima treated with stents implantation. It is suspected that increased-CRP may be a manifestation of acute coronary syndrome.