Abstract:Aim To obtain the whole gene represented by the expressed sequence tag (EST)AW918640 and analyse its elementary characteristic. Methods Alignment, assembling and other bioinformatics programs were used to perform in silicon cloning form GeneBank data-base. RT-PCR and gene sequencing were performed to identify it. Localization in chromosome, sequence of amino acid and functional domain were identified by map viewer, ExPASy and SMART software respectively. Results AW918640 is an EST sequence that expressed highly at 3 h after myocardial ischemic preconditioning in rat. We got the whole nucleotides sequence represented by AW918640 using in silicon cloning. The result from gene BLAST in GeneBank suggested that the whole nucleotides sequence express a new gene. We named this novel gene myocardial ischemic preconditioning associated gene-5 (MIP5, GenBank accession number: AY553870). Furthermore, the nucleotide sequence of MIP5 was confirmed by RT-PCR and sequencing. MIP5 contained a single open reading frame (ORF)of 909 base pairs with the potential to encode a protein of 302 residues containing 6 kelch repeat motif predicted by map viewer, ExPASy and SMART software respectively. Conclusion MIP5 is a novel gene which is correlative with myocardial ischemic preconditioning in rat.

Abstract:Aim To study the change of cellular growth factor receptor and its effect on the proliferation and migration of vascular smooth muscle cells (VSMC) under electrical field. Methods An electrophoresis apparatus was designed for applying the direct current electric fields to the cultured VSMC, which got from the aorta of rat. Interval photographs were used to analyse the direction and distance of VSMC migration. The cellular growth factors such as platelet derived growth factor receptor (PDGFR), angiotensin Ⅱ type 1 receptor (AT1R) and its type 2 receptor (AT2R) were studied by immunohistochemistry or immunofluorescence to show the mechanism relation to the migration of VSMC. Results The longer time of exposure to the 150 V/m electric field, the more electrotaxis the cells showed, that is, the distance of cellular migration increased obviously with the time of exposure to the electric field compared with control group. Under the direct current electric field, the expression of PDGFR were increased obviously and distributed asymmetricly on the membrane of VSMC. This receptor were mainly focused on the cathodal side of cellular membrane in electric field. The AT1R were also enhanced in the cells after exposure to the electric field, and the expression of AT2R had showed the changeless. Conclusion Our study indicates that electric fields applied to the rat VSMC can change the growth characteristic and increase the directly migration of VSMC. These effects maybe relate to the up-regulated expression and re-distribution of some cellular growth factors such as PDGFR, AT1R and AT2R.

Abstract:Aim To explore the effect of coixan on endothelium-1 (ET-1) gene expression of type 2 diabetic model on rat arota. Methods The experimental type 2 diabetic rats were yielded by i.p streptozotocin (75 mg/kg) and feeding hyperotrophic foods,then to observe ET-1 gene expression by RT-PCR. Results After 6 months of i.p streptozotocin, the ET-1 gene expression 0.72±0.10 on rat arotic enhanced obviously, the comparison between normal group 0.39±0.01 (p<0.01), ET-1 mRNA gene expression of administered groups downregulate (p<0.05), and the ET-1 gene expression of coixan i.p group 0.49±0.12 showed more significant difference than the model control group (p<0.01). Conclusion Diabetic vascular complication may be related to upregulation of ET-1 mRNA gene expression,Coixan decreased ET-1 mRNA gene expression.

Abstract:Aim To observe the effect of vitamin C on blood lipid and aorta expreeion of heparin sulfate proteoglycan protein (HSPG) in streptozotocin-induced diabetic rats. Methods Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were allocated to normal control group, model group,and vitamin C group for 16 weeks. During and after the treatment, during and after the treatment, Its general state, blood suger (BS), triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDLC), high density lipoprotein (HDLC), glycosylated hemoglobin (HbA1c) and glycosylated low density lipoprotein (G-LDL) Alteration of tissue morphology via HE staining was observed after the aorta was taken out from the rats executed by narcosis; heparin sulfate proteoglycan protein, were determined by immunohistochemistoche. Results (1) Diabetes mellitus and aortalesion occurred in the four model groups. (2)Vitamin C have no effect on the blood gloucoselevels,while they could improve the general state, improve the general state, decreasetriglyceride (p<0.05), serum cholesterol (p<0.05), low density lipoprotein (p<0.05), glycosylated hemoglobin (p<0.05), and glycated low density lipoprotein (p<0.05), increase high density lipoprotein (p<0.05), Theexpreeion of HSPG in the kidney were significantly inhibited (p<0.05) at the same time. Conclusion Vitamin C have no effect in decreasing the blood sugar level but have a certain in preventing renal.

Abstract:Aim To investigate the effects of C-type natriuretic peptide (CNP) gene transduction on vascular smooth muscle cell proliferation after angioplasty. Methods Eighty-four rabbits were divided into 3 groups equally:normal,control and test group. The control and test group had been given high-cholesterol diet from 7 d pre-experiment to being killed. All rabbits were fed by high-cholesterol diet. Restenosis models were established by injured iliac artery in control group and test group. In control group, alkaline phosphatase gene that retroviral vector carried was transferred at injured site. In test group,CNP gene that retroviral vector carried was transferred at injured site. Iliac arteries injured were harvested for ~3H-TdR incorporation, immunohistochemistry analysis of proliferating cell nuclear antigen (PCNA). Lumen area, neointimal thickness, neointimal area, neointimal area/media area were measured by image analysing system. Results Iliac arteries injured by balloon, 3 days later, neointimal thickness, neointimal area and neointimal area/media area were increased in control group and test group, and the index was markedly increased after 2 weeks later. The index of test group was less than control group (p<0.05 or p<0.01). Incorporation of ~3 H-TdR became higher 3 days later after balloon angioplasty in control group and test group, but there was no significant difference in two groups (p<0.05). ~3H-TdR incorporation of test group was less than control group 1 week later after balloon angioplasty (p<0.05). ~3H-TdR incorporation of test group was near to normal level 4 weeks later after balloon angioplasty (p<0.05), but there was still higher in control group (p<0.01). Immunohistochemistry staining of PCNA suggested that large numbers of PCNA-positive cells were seen at intima layer 2 weeks later after balloon angioplasty in control group, but there were no conspicuous express in test group. Conclusion Local CNP gene transduction could effectively inhibit vascular smooth muscle cell (VSMC) proliferation and intimal hyperplasia after angioplasty.

Abstract:Aim To study traditional Chinese herbal compound reverse myocardial fibrosis after acute myocardial infarction (AMI) in rats. Methods AMI model was established by ligature of left anterior descending coronary(LAD). All of the models were divided into 6 groups at random with ten in every group: group M_ 14 (without any treatments, killed at the 14 th day after infarction), group M_ 56 (without any treatments, killed at the 56th day after infarction), group T_ 14 (treated with herbal compound for 14 days before kill), group T_ 56 (treated with herbal compound for 56 days before kill), S_ 14 (sham operation, killed at the 14th day after surgery), and group S_ 56 (sham operation, killed at the 56th day after surgery) in 60 male SD rats. 24 hours after operation, the herbal compound (10 mL/kg·day) was given for 14 days or 56 days. Results Body weight (BW) of all the rats had non-significant difference (p<0.05). There were not significant differences in heart weight (HW), left ventricle weight (LVW), HW/BW, LVW/BW between group M_ 14 and S_ 14 (p<0.05), but these indexes in group M_ 56 (separate 1 054±22 mg, 850±21 mg, 2.77±0.08, 2.23±0.04) were higher than those in group S_ 56 (990±14 mg, 786±14 mg, 2.58±0.04, 2.05±0.03)(p<0.01). HW, LVW, HW/BW, LVW/BW in T groups were lower at the 14 th and the 56th day (separate T_ 14 778±13 mg,607±11 mg, 2.54±0.04, 1.98±0.03; T_ 56 1 031±14 mg, 822±14 mg, 2.65±0.04, 2.11±0.02) than those in M groups at corresponding time (p<0.01). Collagen contents of T groups (separate T_ 14 4.37±0.57 mg/g, T_ 56 6.04±0.69 mg/g) were lower at the 14th and 56th day than those of M groups (separate M_ 14 5.22±0.52 mg/g, M_ 56 4.37±0.57 mg/g) (p<0.01), but were still higher than those of S group (separate S_ 14 3.65±0.41 mg/g, S_ 56 4.33±0.51 mg/g)(p< 0.01 ). A great deal of collagen fibers deposit in infarct zone and non-infarct zone and collagen deposition declined obviously after treatment of herbal compound under histopathologic observation. Conclusion Herbal compound can reverse myocardial fibrosis to some extent.

Abstract:Aim Take coronary heart disease patient's peripheral blood mononuclear cells (PBMC)as the study object, observing the effect of niacin to the expression of cyclic adenosine monophotosphate (cAMP)and adenosine triphosphate binding cassette transporter A1 (ABCA1) in PBMC which incubated in vitro, analysing the correlation of niacin adjusts the expression of ABCA1 and cAMP/ protein kinase A (PKA) pathway. Methods To detect the expression of ABCA1 mRNA and protein by reverse trancriptase-polymerase chain reaction (RT-PCR) and western blot, to determine the level of cAMP in PBMC by low pH EIA kit. Analysing the expression of ABCA1 change and cAMP level change, discuss the correlation on the expression of ABCA1 and cAMP in PBMC by the role of niacin. Results (1)Each group of the expression of ABCA1 and the cAMP level changes in PBMC in 3 h and 48 h does not have the obvious difference (p<0.05). (2)The expression of ABCA1 of coronary heart disease high blood fats patient in PBMC compares the normal person obviously to reduce (p<0.05), but in the normal blood fats group the expression of ABCA1 in PBMC compares the normal person not to have the obvious change (p<0.05). (3)The niacin alone affects obviously increase in coronary heart disease person uninuclear cell the ABCA1 expression (p<0.05), at the same time makes the cAMP content in the PBMC to increase (p<0.05). (4)PKA inhibitor Ro 31-8220 alone affects can obviously reduce in coronary disease person he expression of ABCA1 in PBMCs (p<0.05),at the same time causes the cAMP content in PBMCs to reduce (p<0.05). (5)In the niacin +PKA inhibitor group were preincubated for 30 minutes in the presence of PKA inhibitor Ro 31-8220 (900 nmol/L), before niacin (1 000μmol/L)was added. then the niacin increases the expression of ABCA1 and the cAMP content in PBMCs was suppressed completely (p<0.05). Conclusion According to the result analysis of the experiment, the effect that niacin affect the expression of ABCA1 through the pathway of cAMP/ PKA, and there is an explicit correlation that niacin increase the expression of ABCA1 in PBMCs with the pathway of cAMP/PKA.

Abstract:Aim To investigate the effect of testosterone on endothelial cells in male rabbits atherosclerotic models. Methods Male cholesterol-fed rabbits were divided into four groups at random: placebo group (castrated rabbits treated with placebo), group with low level testosterone (castrated rabbits treated with testosterone 0.25 mg/kg·d), group with high level testosterone (castrated rabbits treated with testosterone 2.5 mg/kg·d), sham operation group. Three months later, the rabbits were sacrificed. Testosterone level, lipids, plasminogen activator inhibitor activity (PAI), nitric oxide (NO), endothelin (ET) and angiotensinⅡ (AngⅡ) concentration were tested. And the ratio of aortic plaque area to total intima area was examined. Results It showed that testosterone level of placebo group was lower than those of the other groups (2.56±0.28 μg/L vs 7.67±0.61 μg/L, 40.16±0.93 μg/L, 7.79±0.61 μg/L, p<0.01); NO content of placebo group was lower than those of the other groups (39.0±9.5 μmol/L vs 62.8±8.9 μmol/L, 56.7±10.3 μmol/L, 51.5±9.3 μmol/L, p<0.05 or p<0.01); PAI of placebo group was higher than that of group with low level testosterone (567.3±53.4 u/L vs 334.4±51.3 u/L, p<0.01); ET of placebo group was higher than that of group with low level testosterone (911.9±15.4 ng/L vs 846.1±43.0 ng/L, p<0.05); and AngⅡ of placebo group was higher than that of group with low level testosterone (687.2±36.5 ng/L vs 624.5±54.5 ng/L, p<0.05); the ratio of aortic plaque area to total intima area of placebo group was higher than others (46.4%±9.3% vs 20.6% ±5.3%, 22.7%±4.6%, 28.5%±13.8%, p<0.01). Conclusions Testosterone is a protective factor for endothelial cells, thus to alleviate atherosclerotic lesion for male rabbits fed with high-cholesterol stuff.

Abstract:Aim To investigate the expression of matrix metalloproteinases-9 (MMP-9), transforming growth factor-beta1 (TGF-β1) and TGF-beta receptorⅠ (TβR-Ⅰ)in human atherosclerotic plaque and the relationship between them and plaque stability. Methods 41 specimen of human coronary artery atherosclerotic plaque were obtained from patients undergoing cardiovascular surgery, and then were routine paraffin embedded, sectioned at 4 μm, and stained with hematoxylin and eosin. They were divided into stable (with no or only little lipid core) and unstable plaques (with lipid core size >40%) according to the structure under the opticalmicroscope, the qualitive and quantitive analyses of MMP-9, TGF-β1 and TβR-Ⅰ were performed by using immunohistochemical staining technique and auto picture analysis system. Results The immunopositive stain of MMP-9, TGF-β1 and TβR-Ⅰwere all located in the vascular smooth muscle cells, endothelial cells, fibroblast, and particularly in the foam cells and macrophage in the atherosclerotic plaque tissue. The protein expression of MMP-9 in the unstable plaques was much stronger than that in the stable ones (average area density: 0.21±0.04 vs 0.16±0.02, average absorbent values: 3.48±0.65 vs 2.84± 0.27 , p<0.01), and was also stronger on the shoulder of the plaque, in the foam cells and macrophage around the lipid core, while the protein expression of TGF-β1 was much stronger in the stable plaques (average area density: 0.23±0.05 vs 0.17± 0.02 , p<0.01; average absorbent values: 3.60±0.55 vs 3.16±0.65, p<0.05). There were no significant difference for immunopositive stain of TβR-Ⅰbetween the stable and unstable plaques. Correlation analysis showed that there was a negative correlation between the expression of MMP-9 and TGF-β1 with both average area density and average absorbent values (r= -0.332 , P=0.034 for average area density; r=-0.373, P=0.016 for average absorbent values). Conclusions There was a close relationship between MMP-9,TGF-β1 and plaque stability. Enhanced production MMP-9 may participate in the formation of unstable plaque, while TGF-β1 may be an important stabilizing factor to prevent the transition into an unstable plaque phenotype.

Abstract:Aim To investigate the expression of matrix metalloproteinase-9 (MMP-9) and matrix metalloproteinase-2 (MMP-2) in rats following intracerebral hemorrhage(ICH). Methods ICH model was produced by stereotactically infusing 75 μL autologous fresh blood taken from rat femoral artery into the left caudate nucleus. The expression of MMP-2 and MMP-9 was detected by zymogram analysis over a time course ranging from 6 hours to 15 days. Results The expression of MMP-2 and MMP-9 in the hemorrhage site increased progressively over time after ICH. After 6 hours, significant (p<0.05) MMP-9 activity reached maximum levels by 24 hours (p<0.01), then persisted for 3 days at this level and returned based level (zero) by 15 days. MMP-2 was significantly (p<0.05) increased by 24 hours and was maximum after 5 days following ICH. Conclusions These results demonstrated that early increased MMP-9 expression was a significant responses to ICH; MMP-2 may contribute to brain tissue repairing.

Abstract:Aim To evaluate the effect of adrenomedullin and tumour necrosis factor-αvariation in the coronary circulation in coronary heart disease patients during percutaneous coronary intervention on restenosis. Methods Blood samples of 70 patients with coronary heart disease were obtained from both the coronary sinus and femoral artery before and after angioplasty, immediately after percutaneous transluminal coronary angioplasty. Plasma adrenomedullin, tumour necrosis factor-αlevels were measured by radioimmunoassay. Coronary angiography was performed again at 6 months after percutaneous coronary intervention or having evidents of myocardial ischemia. Results Restenosis occurred in 21.4% (15/70) of patients. Before percutaneous coronary intervention, the adrenomedullin, tumour necrosis factor-αlevels in coronary sinus were no significant differences in patients with or without restenosis. However, adrenomedullin and tumour necrosis factor-αlevels after percutaneous transluminal coronary angioplasty in patients with restenosis (40.1±1.1 pg/L and 15.0±0.4 pg/L) were higher than those without restenosis (35.8±1.6 pg/L and 10.6±0.4 pg/L, all p<0.01). Conclusions There was a high incidence of restenosis in patients with a increasing adrenomedullin、 tumour necrosis factor-αlevels in coronary sinus after percutaneous transluminal coronary angioplasty. It shows that adrenomedullin、tumour necrosis factor-αlevels in coronary sinus after percutaneous transluminal coronary angioplasty have a close relationship with restenosis and may become clinical prognostic indicator of estimating restenosis.

Abstract:Aim To investigate the changes of circulating endothelial cells (CEC), nitric oxide (NO), oxidized low density lipoprotein (ox-LDL), high-sensitivity C-reactive protein (Hs-CRP) and manifestation of acute coronary syndrome (ACS). Methods The plasma Hs-CRP levels were measured by using particle enhanced immunonephelometry and the plasma ox-LDL levels were measured by enzyme linked immunosorbent assay (ELISA) in 20 normal control subjects and 51 patients with ACS. The plasma CEC and NO levels were also determined. The linear correlation was analyzed to estimate the changes of these variables. Results The levels of ox-LDL, Hs-CRP, CEC in ACS patients were markedly higher than those in control group respectively (686±168 μg/L vs 349±172 μg/L, p<0.01), (6.15±1.75 mg/L vs 1.53±1.64 mg/L, p<0.01), [(8.9± 1.6) ×10~6 cells/L vs (2.4±0.4) ×10~6 cells/L, p<0.01]. The level of NO in ACS patients was significantly decreased compared with control group (52.2±16.5 μmol/L vs 77.3±21.0 μmol/L, p<0.05). The linear correlation analysis showed that the level of ox-LDL was correlated positively with CEC (r=0.781, p<0.001), and NO (r=0.792, p<0.001) in patients with ACS. The level of Hs-CRP was correlated positively with CEC (r=0.776, p<0.001) and NO (r=0.897, p<0.001) in patients with ACS. The level of ox-LDL was also positively correlated with CRP in patients with ACS (r=0.768, p<0.001). Furthermore, the level of CEC was positively correlated with NO in patients with ACS (r= 0.951 , p<0.001). Conclusion Ox-LDL and CRP may participate in endothelial dysfunction, which contributes to pathogenesis of ACS.

Abstract:Aim To investigate the effect and security of Cypher~ TM stent (sirolimus-eluting stent) in patients with coronary heart disease (CHD). Methods 348 CHD cases were treated with 436 Cypher~ TM stents. Among the 348 patients, 86 had ST-segment elevation acute myocardial infarction (AMI), 21 had non-ST-segment elevation AMI, 149 had unstable angina and 92 had stable angina. As for lesion characteristics, diffuse disease was found in 88 cases (25.3%), bifurcation lesions in 62 cases (17.8%), chronic total occlusion lesions in 55 cases (15.8%), in-stent restenosis in 15 cases. The immediate angiographic outcome, six months follow-up results with major cardiac event (MACE) and angiographic follow-up at 6 months had been assessed. Results A successful stenting procedure was achieved in 99.3% patients with CHD. Acute and sub-acute stent thrombosis occurred in 2 patients, late stent thrombosis with AMI occurred in 1 patient. There was 1 case death during the 6 months follow-up. The MACE rate during hospitalization was 0.6% and during 6 months follow-up was 2.9%. Angiographic follow-up at 6 months showed that in-stent restenosis rate (ISR) was 7.1% (restenosis within the stents was 1.8%), the late loss in lesion was 0.16 mm (in-stent was 0.20 mm). The target vessel revascularization (TLR) rate was 5.4%. Conclusion Cypher~ TM stent implantation in CHD is safe and effective, and the ISR rate and TLR rate are significantly lower than that of bare metal stents.

Abstract:Aim To evaluate the effect of aspirin time-release on the incidence of cerebro-cardiovascular accidents in hypertensive patients. Methods This study was an opening, randomized, and controlled clinical prospective research, participated by 13 “the 3rd degree and grade A” hospitals and “the 2nd degree and grade A” hospitals which were organized by the cerebro-cardiovascular disease interventional net of hunan province coordinating center. 2 274 patients were randomly assigned to treatment group and control group. There are 1 286 patients in treated group assigned to aspirin 50～100 mg/d besides anti-hypertensive treatment, while 1 088 patients in control group received only routine anti-hypertensive therapy. The average follow-up time was 3.2 years (range 1～4 years). Finally 2 180 patients had completed the study which included 1 151 treated group patients and 1 029 control group patients. Results In treated group, the cumulative incidence risk of total cerebro-cardiovascular accidents, cerebral infarction, cerebral hemorrhage, myocardial infarction, total death, death caused by cerebro-cardiovascular accidents was 3.6%, 2.1%, 0.7%, 0.2%, 0.4%, 0.2%, but in control group the cumulative incidence risk of each event was 5.2%, 3.6%, 1.3%, 0.9%, 0.4%, 0.3%. The study observed that active treatment obviously reduced the incidence of fatal and non-fatal acute myocardial infarctions (p<0.05), compared with the controll group, the relative risk (95% confidence interval,95% CI) was 0.204 (0.044～0.943). But the relative risk of cerebro-cardiovascular accidents, cerebral infarction, cerebral hemorrhage was 0.701 (0.469～1.048), 0.815 (0.470～1.412), 0.567 (0.235～1.368), respectively, which are nonsignificant. Furthermore, the relative risk of death caused by cerebro-cardiovascular accidents was 3.645 (0.407～32.614). Conclusions The active therapy with low-dose aspirin could significantly reduce the incidence of fatal and non-fatal acute myocardial infarctions in hypertensive patients. And there was no increased risk of stroke or bleeding. So it has effect on the primary prevention.

Abstract:Aim To investigate the effect of fluvastatin on expression of matrix metalloproteinase-9 (MMP-9), tissue factor (TF) from the peripheral blood monocytes in patients with acute myocardial infarction (AMI). Methods Monocytes from 30 AMI patients and 30 control subjects were separated and added into two bores, one cultivated directly, one cultivated with fluvastatin (360 μg/L). The contents of MMP-9, TF were assayed by enzyme linked immunosorbent assay (ELISA). Results Compared with control noninterfering group, the productions of MMP-9, TF in AMI noninterfering group were increased significantly (351±141 μg/L vs 121±10 μg/L, p<0.01; 41.4±13.6 ng/L vs 25.5±5.4 ng/L, p<0.01). In AMI group, compared with the noninterfering group, the productions of MMP-9, TF in fluvastatin group have been decreased significantly (226±89 μg/L vs 351±141 μg/L, p<0.01; 31.4±11.2 ng/L vs 41.4±13.6 ng/L, p<0.05). Conclusion The productions of MMP-9, TF in monocytes from patients with AMI were increased. Fluvastatin inhibited the expression of MMP-9 and TF.

Abstract:Aim To study the clinical effect of Shuxuening injection on senile cerebral infarction. Methods 118 cases of senile cerebral infarction were randomly divided into two groups: 58 cases in treatment group were treated with Shuxuening injection, 60 cases in control group were treated with compound Salvia injection. Hemorrheological indexes were checked before and after therapy, the neurological deficit scale and curative effect of the two groups were compared. Results Hemorrhelogical indexes were significantly different in treatment group in using Shuxuening injection before and after therapy. In comparison with control group, hemorrheological indexes such as plasma viscosity, fibrinogen concentration of plasma, deformation index of red blood cell (RBC) and aggregation index of RBC were significantly different(p<0.05). The neurological deficit scale in treatment group were significantly lower than those in control group on the 14 th day after treatment, and more on the 21th day (p<0.01). On the 14 th day after treatment, the total effective rate of treatment group and control group was 86.2% and 68.3%. There is obvious difference in two groups of curative effect(p<0.05). Conclusions Shuxuening injection is efficient in treating senile celebral infarction just with very slight adverse effects, and provides selectable chance in the treatment of senile cerebral infarction.

Abstract:Aim To study the dynamic change of postprandial lipid after a fat meal in patients of normotriglyceridemic type 2 diabete. Methods Subjects were assigned to two groups:the observed group of 25 fasting normotriglyceridemic type 2 diabetic patients; the healthy control group of 20 normal subjects. Postprandial lipids were measured after a fatty test meal. Results Postprandial TG concentrations of the two groups are all raised. TG peak times were all approximate 4 h in the two groups. TG concentrations at 8 h were significantly higher than fasting TG levels in case group (p<0.05), but were no significantly different in control group (p<0.05). A slight postprandial decrease in HDL-C was observed in both groups with no significant difference between groups nevertheless, and there were no difference between diabetics and controls. Postprandial changes in total cholesterol and LDL cholesterol were weak after the test meal. The correlative coefficients of TG-AUC, TG-IAUC and TG levels at 4 h were the biggest (p<0.05). Conclusions The postprandial dyslipidemia usually appears before fasting dyslipidemia in patients with type 2 diabetes mellitus. It is necessary to measure fasting TG level and 4 hour TG level to see if the triglyceride metabolism is abnormal.

Abstract:Aim To investigate the clinical applicative value of multislice spiral CT angiography (MSCTA) in the diagnosis of aortic dissections (AD). Methods MSCTA was performed on 21 patients with AD. The computed tomography was carried out with scanning thickness of 2.5 mm, reestablishing interval of 1.6 mm, pitch of 1.25, control reagent of 100 mL in an infusing speed of 3 mL/s, and a delay time of 18～20 s. All data were imported into the workstation and analyzed by maximum intensity projection (MIP), multiplanner reformation (MPR), shadow surface display (SSD) and volume rendering (VR). Results Among the 21 patients, there were 5 cases for Debakey type Ⅰ, 3 for type Ⅱ and 13 for type Ⅲ. All the images by MSCTA showed the whole length of aorta, real and false cavities, endothelium layer and aortic aneurysm with a very good quality. Conclusion MSCTA is of important clinical value in the diagnosis and treatment of AD.

Abstract:Aim To study the characteristics of lipid level phenotyping of Shenyang professional populations. Method 3 919 cases (M/F=2 134/1 785, aged 40～89)were enrolled in the study. Lipid levels were classified according to the “recommended guidelines for prevention and treatment of dyslipidemia" of China (1997)and NCEP ATP Ⅲ(2001)of US. Results High prevalence of lipid abnormality was found in Shenyang populations,only 40% had desirable total cholesterol (TC)and triglyceride (TG)level in the group with age above 50. There are 46.7% of the 40～49 aged men and 34.2% 40～49 aged women, 45.1% of the 50～59 aged men and 53.1% 50～59 aged women, 51.1% men above 60 and 63.0% women above 60 who have TC levels above 5.2 mmol/L. And more than 10% of the men have TC levels above 6.2 mmol/L. 18.2% of the 50～59 aged women and 25.8% of the women above 60 also have TC levels above 6.2 mmol/L. Hyperlipoproteinemia high TC was about twice more than high TG. Decreased high density lipoprotein cholesterol (HDLC)was found in 15% male subjects, but only 5% in females. The prevalence of high level HDLC was found in 31.9%～47.3% of female groups, and 16.5%～21.9% in male groups. Conclusion The prevalence of lipid abnormality was more than 50% of the studied subjects above age 50, which means that the atherosclerosis disease risk is high in this population. Lipid modification may be indispensable.

Abstract:Aim To investigate the prevalence and distribution of metabolic syndromein the elder male community population of Beijing. Methods The medical check-up data in 1 736 elder male cases,which sampled randomly from the 136 communities in Beijing,were acquired and analyzed. Results The proportions of theelder male people with metabolic syndrome, obesity, essential hypertension, glucose metabolic disorder, triglycerodes were 30.7%, 39.6%, 56.4%, 62.0% and 37.0% respectively. In the old patient's groups, the prevalence rates of MS and essential hypertension were similar to that of very old patients', while the glucose disorder werelower, obesity and triglycerodes were significantly higherthan that of very old patients' group. The prevalence of MS risk population in the very old patients' group was also significantly higher than that of the old patients' group. Conclusion The prevalence rate of MS is very high in theelder male community population of Beijing. The rates of MS and its components withthe middle aged patient's groupdiffered from those ofthe old and very old patient's groups.

Abstract:Aim To observe the changes of hemorheology in the patients with cerebral infarction after hyperbaric oxygen (HBO) therapy. Methods 64 patients with cerebral infarction were randomly divided into the control group (drugs treatment only in 32 cases) and hyperbaric oxygen therapy group (hyperbaric oxygen plus drugs treatment in 32 cases), the hemorheological indexes were dectected before treatment and 12 days after treatment. Results The hemorheological indexes in hyperbaric oxygen therapy groups were all improved, those in hyperbaric oxygen therapy group after treatment were close to the normal level. While the hemorheological indexes in the control group were partly improved. Which were not more prominent than those in hyperbaric oxygen therapy group. Conclusion Hyperbaric oxygen therapy can improve the hemorheological indexes, according to the hemorheology to judge the curative effect of hyperbaric oxygen therapy.