Abstract:Aim To study development coronary artery Atherosclerosis and coronary artery spasm model in Chinese mini-swine mediated by inflammatory factor.Methods Chinese mini-swine were randomly divided into three groups.For establishing coronary artery atherosclerosis and coronary artery spasm model,chest operation was done by isolation of left-anterior descending banch and circumflex branch proximate.Coronary artery membranna externa in swine was packed by paper towel absorbing saline agarose particle suspension in control group.Coronary artery membranna externa in swine was packed by paper towel absorbing IL-1β(2.5 μg) agarose particle suspension in model 1 group.Coronary artery membranna externa was packed by paper towel absorbing IL-1β(6.0 μg)agarose particle suspension in model 2 group.After 2 weeks the state of coronary artery spasm wass induced by injecting 5-serotonin(3 μg/kg,10 μg/kg) and histamine(3 μg/kg,10 μg/kg)into coronary artery via conduct.Lumens narrow was observed by coronary arteriogramphy.Results Lumens narrow and CAS in model 1 group were observed.Positive rate of coronary artery lumens narrow was 20～30%,positive rate of CAS was 82%.CAS in model 2 group was not observed.The pathological examination showed that the macrophage accumulation could be found at different degrees in both groups.Conclusions 5-Hydroxytryptamine and histamine can induce CAS,Atherosclerosis and lumens narrow produce in coronary artery membrana externa packed by IL-1β.

Abstract:Aim To investigate the relationship between cytokines expression and inflammatory response after myocardial infarction in mice.Methods Develope mice model of myocardial infarction by coronary artery ligation.Levels of serum tumor necrosis factor-α,interleukin-6 and interleukin-10 were assayed by ELISA,and pathology of myocardium was detected by histochemistry.Results Expressions of tumor necrosis factor-α(82.4±27.6 ng/L) and interleukin-10(47.7±22.3 ng/L) were found to be significantly higher in 1 h after myocardial infarction than that of sham operated groups(40.4±9.7 ng/L and 23.4±4.3 ng/L),and they decreased gradually after that;Expressions of interleukin-6(202.7±89.5 ng/L,578.9±198.0 ng/L,696.8±104.7 ng/L,325.1±131.7 ng/L,218.5±16.0 ng/L,and 195.8±93.1 ng/L) were also found to be significantly higher in 1 h,2 h,4 h,1 d,2 d,and 5 d after myocardial infarction than that of sham operated groups(54.0±3.0 ng/L).Leucocytes recruited in the infarcted myocardium after myocardial infarction.Inflammatory cytokines decreased during 2 weeks and 4 weeks after myocardial infarction,and Leucocytes in the infarcted myocardium decreased gradually.Myocardium fibrosis was found.Conclusion The changes of tumor necrosis factor-α,interleukin-6 and interleukin-10 take part in the inflammatory response after myocardial infarction,and play an important role in the development of myocardial infarction diseases.

Abstract:Aim To investigate the effect of combined gene transfer of angiopoietin-1(Ang-1) gene and vascular endothelial growth factor(VEGF) gene,with different proportions,on angiogenesis,arteriogenesis and neovascular structure and function in a rat model of hindlimb ischemia.Methods Expression plasmid,pcD2(control plasmid),pcD2/Ang-1,pcD2/VEGF,or pcD2/Ang-1+pcD2/VEGF at different proportions,was injected intramuscularly into ischemic hindlimb rat model,followed by electroporated.VEGF and Ang-1 expressions were evaluated by RT-PCR.Angiogenesis and arteriogenesis were assessed by capillary density,arteriole density and angiography.Vascular permeability was evaluated by Evans blue uptake assays.Results Collateral vessel development was assessed at 14 days after treatment.Vessel numbers increased slightly in the rat transfected with pcD2/Ang-1 or pcD2/VEGF compared with that in the rats transfected with pcD2 alone(p<0.05).In the rats transfected with pcD2/Ang-1+pcD2/VEGF,with the increase of pcD2/Ang-1 during the pcD2/VEGF at 100 μg,the number of artery increased and was 1.9,2.4 and 2.45 times higher than that of control rats after gene delivery.With the increase of pcD2/VEGF during the pcD2/Ang-1 at 100 μg,the number of artery increased and was 2.9,2.07 and 2.39 times higher than that of control.Evans blue amount in skeletal muscle increased companied with the dosages of pcD2/VEGF.Combination with pcD2/Ang-1 decreased the vessel leakage.Evans blue uptake in group of pcD2/VEGF 100 μg+pcD2/Ang-1 200 μg was the closest to that of the normal group(p>0.05).Conclusions The current study demonstrated that co-expression of Ang-1 and VEGF genes in the ischemic muscle effectively develop leakage-resistant vessels in rat model.At a ratio of pcD2/VEGF to pcD2/Ang-1 by 1:2 is the optimal dosage.Therefore,this approach may provide a more appropriate therapeutic strategy in ischemic vascular diseases.

Abstract:Aim To investigate the regulation of X-box binding protein-1 (XBP-1) and acyl-CoA cholesterol acyltransferase-2 (ACAT-2) expression by cholesterol in hepatic cell and enterocyte. Methods HepG2 cell and Caco2 cell were incubated with different concentration of free cholesterol and oxidative cholesterol. The mRNA level of XBP-1 and ACAT-2 in cells were detected by semi-quantitative RT-PCR. Results In cells incubated with free cholesterol and oxidative cholesterol, XBP-1 and ACAT-2 mRNA levels in HepG2 cell and Caco2 cell were up-regulated, and were dependent on the concentration increase. Conclusions Both of cholesterol and oxidative cholesterol can induce UPR, and ACAT-2 mRNA level can be up-regulated, the results imply the possibility that ACAT-2 may be regulated by UPR; in view of ACAT-2 playing an important role in the cholesterol esterification and absorption, UPR may have important significance in cholesterol absorption.

Abstract:Aim To investigate the effect of angiotensin Ⅱ(AngⅡ) and its receptor antagonist on the matrix metalloproteinases 2(MMP-2) mRNA and protein expression in double stable AT2R expression cell lines of VSMC.Methods To establish the double stable cells of rat vascular smooth muscle cells(VSMC),in which the expression levels of AT2R is tightly controlled by doxycycline(Dox).The VSMC were treated with AngⅡ,Dox,CV-11974,PD123319.The effect of overexpression of AT2R on the mRNA and protein expression of MMP-2 in VSMC were detected and the effects of AT1R antagonist(CV-11974) and AT2R antagonist(PD123319) on aforementioned target were studied.Results The doxcycline(Dox) on gene expression system can generate high level and regulable expression of AT2R which tightly controled by addition/removal Dox.The expression of AT2R were induced obviously in double stable VSMC within 48 h by addition Dox and further,which enhanced after 72 h(p<0.01).In the well established double stable VSMC lines,addition of AngⅡ resulted in an increase of the expression of the MMP-2(p<0.01).The MMP-2 expression was decreased by treatment with Dox(p<0.01).Dox-induced decreased expression of MMP-2 were further enhanced by CV-11974(p<0.01),and were removed by PD123319.There are no difference in expression of MMP-2 between CV-11974,PD123319 simultaneously group with control group.Conclusion The enhancement effect of AngⅡ on the expression of MMP-2 is mediated by AT1R and inhibited by AT2R expression.It is suggestded that the regulable expression of AT2R can control the synthesis and degradation of Extracellular matrix effectively.

Abstract:Aim To study the effects on the proliferation of ramification of Curcumin hydrolyzed by HCl(RCH) on the bovine vascular smooth muscle cell(VSMC) and the expression of low density lipoprotein receptor(LDLR).Method Curcumin was hydrolyzed by HCl,the proliferation models of the bovine VSMC were stimulated by ox-LDL,MTT was used to observe its effects on proliferation of the bovine VSMC.FCM was used to observe its effects of expression of LDLR.The c-myc mRNA was measured by RT-PCR.Results Ramification of Curcumin Hydrolyzed have obvious effect on anti-proliferation of VSMC stimulated by 10 mg/L ox-LDL(p<0.01).And it can obviously increase the expression of LDLR of VSMC and down-regulate the expression of c-myc.Conclusion Ramification of Curcumin hydrolyzed have obvious effect on anti-proliferation of VSMC,and it can increase the expression of LDLR.That may be the mechanism of delaying the development of arteriosclerosis.

Abstract:Aim To study the effects of probucol on ATP binding cassette transporter A1(ABCA1) expression induced by oxidized low density lipoprotein(ox-LDL) in THP-1 macrophages.Methods Reverse transcription polymerase chain reaction(RT-PCR) and immunofluorescence were used to detect ABCA1 mRNA and protein expression respectively.Results ABCA1 mRNA levels in THP-1 macrophages increased in time-dependence pattern after cells incubated for 0 h,12 h,24 h and 48 h with 100 mg/L ox-LDL.After pretreation for 4 h with 50 μmol/L probucol,ABCA1 protein expression induced by ox-LDL for 48 h attenuated,with no change of ABCA1 mRNA levels in THP-1 macrophages.Conclusion Probucol downregulated ABCA1 protein expression induced by ox-LDL.

Abstract:Aim To investigate dynamic changes of water content and neuropetie Y(NPY) in intracerebral hemorrhagic(ICH) rats and the role that neuropetie Y plays in cerebral edema after hemorrage,and to evaluate the effect of aprotinin in reducing the content of cerebral edema and neuropetie Y.Methods Animal model was made by injecting self arterial blood into the caudate nucleus of rats.75 rats were randomly assigned to control group,cerebral hemorrhage group and aprotinin treated group.After the injection,the rats were sacrificed at 6 h,1 d,3 d,5 d,7 d respectively.Brain water content was determined by wet-dry weight,as the expression of neuropetie Y was detected by radioimmunoassay method over a time course ranging from 6 hours to 7 days.Results Brain water content and neuropetie Y increased after 6 hours of cerebral hemorrhage and reached its climax at the 3 days,then decreased at the 5 to the 7 days;Administration of aprotinin could reduce the content of brain edema and neuropetie Y.Conclusions Aprotinin can reduce neuropetie Y expression,thus can reduce cerebral edema after ICH.Aprotinin antagonist might be available for the therapy of cerebral edema after ICH.

Abstract:Aim To observe the effect of testosterone undecanoate(TU) on intima restoration of iliac artery after injury in male rabbit.Methods Twenty-five male Japanese rabbits were randomized into 5 groups(5 in each):control group,castrated group,small dose group(3 mg/kg),middle dose group(6 mg/kg) and large dose group(12 mg/kg).All five groups of rabbits were castrated except the control group.At the fourteenth day after castration all animals were performed with endothelium denudation at the right iliac artery.At the seventh,fourteenth and twenty-first day after castration,the last three groups of rabbits were intramuscularly injected with testosterone undecanoate,whereas control group and castrated group were intramuscularly injected with 0.9% saline.Blood sample were collected for determination of total testosterone(TT) at different time.At the twenty-eighth day after castration,all animals were killed by air embolism.The endothelial structure and morphological changes were observed with scanning electron microscope and HE staining.Results The hyperplasia of endometrium was serious in castrated group,small dose group and middle dose group at the sight of light microscope,whereas in large dose group this hyperplasia is as light as that in control group.The intima-to-media ratio of iliac artery in castrated group,small dose group and middle dose group(respectively 1.25±0.10,0.90±0.10,and 0.74±0.11) were obviously larger than those in control group(0.22±0.05)(p<0.01),whereas no significant difference was found between large dose group(0.26±0.07) and control group(p>0.05).Scanning electron microscopy showed that the endothelium of control group was smooth and continuous,and the cells were in good order.Whereas in castrated group almost no endothelial cell was observed.There were a few isolated areas having endothelial cells in small dose and middle dose groups.In large dose group,the arrangement of endothelial cells got close to normal state and have small deletion occasionally.At the twenty-eighth day after castration,serum level of total testosterone(TT) in large dose group was similar to that before castration and in control group.Serum level of TT in other groups was obviously lower than that in control group.Conclusion Testosterone,at physiological level,can protect vascular endothelium and inhibit intima hyperplasia in male rabbit.

Abstract:Aim To investigate whether atorvastatin dose-dependently affects endothelial progenitor cells(EPC) mobilization from peripheral blood,EPC numbers in bone marrow culture in vitro and endothelial function after myocardial injury rats.Methods Rats with myocardial injury,induced by isoprenaline,were randomized to treatment groups with vehicle or atorvastatin [5,10,20,40 and 80 mg/(kg·d)] for 4 weeks starting on one day after Myocardial injury.Putative precursor populations [CD34+/vascular endothelial growth factor receptor 2(VEGFR-2)+ haematopoietic stem cells] were measured by flow cytometric analysis.Total mononuclear cells(MNC) were isolated from peripheral blood by Ficoll density gradient centrifugation,and the cells were plated on gelatin coated culture dishes.Double-stained cells for both FITC BS-1 lectin and acLDL-DiI were counted as EPC in at least 3 randomly selected HPF.Serum concentration of nitric oxide(NO) was measured after atorvastatin use.Results Atorvastatin increased EPC numbers both in bone marrow and peripheral blood culture in vitro,maximum at 40 mg/(kg·d)(p<0.05).EPC numbers at the dose of 80 mg/(kg·d) were slightly reduced comparing with 40 mg/(kg·d),but there was no statistical difference;circulating EPC(double positive CD34+/VEGFR-2+) were significantly elevated after myocardial injury(p<0.05);EPC mobilization was markedly further augmented by atorvastatin treatment,maximum at 80 mg/(kg·d)(4.18-fold increase,p<0.05).There was no statistical difference between 40 mg/(kg·d) and 80 mg/(kg·d);Atorvastatin induced a dose-dependent increase in serum concentration of NO generation,with maximal effect at 80 mg/(kg·d).Conclusions Atorvastatin dose-dependently improve EPC mobilization,EPC numbers in vitro culture and endothelial function.

Abstract:Aim To investigate the changes of biomechanical properties of carotid atherosclerosis in rabbits and its connection with the content of collagen.Methods 30 male Japanese White rabbits were randomly divided into control group(n=6) and the surgery group(n=24).The control group was fed by normal diet,but the surgery group was fed by high-cholesterol diet and treated in left carotid artery by air-drying.Meanwhile right carotid artery was treated as sham group.At 2,4,8 and 12 week after operation,the biomechanical properties were measured on a tensile-testing machine.The Elastic modulus,the strength failure and the peak load was measured,meanwhile the degree of artery stenosis was showed by DSA.The stenosis rate,mean integra optical densities(IOD) of media collagen were calculated.Results The typical atherosclerotic plaque showed in surgery group.Following development in degrees of the atherosclerosis,the intima showed proliferation and media showed atrophy and became thin,a mass of collagen tissue proliferated and filled in the smooth muscle cell.Atery elastic modulus and stiffness was increased and atery compliance was decreased.Statistic analysis showed significant difference at the 12 week group,compared with sham group and 2,4 and 8 week group after operation,but it showed no such significant difference at 2 week group after operation,compared with sham group.Conclusions At the early carotid atherosclerosis,vessel showed positive remodeling.Elastic modulus decrease to adapt to the new mechanical condition.But following development in degrees of the atherosclerosis,the content of the artery wall collagen increases,the vessel wall elasticity decrease and stiffness increases.The vessel occurs negative remodeling.It suggests that the interventional treatment be in difficulty.

Abstract:Aim To evaluate the relationship between inflammation and coagulation in the pathogenesis of atherosclerosis.Methods The expression and distribution of tissue factor(TF) and interleukin-18(IL-18) in the human normal coronary arteries and atherosclerotic plaques were studied using HE staining and immunohistochemistry.Results The expression of TF was not detected in the endothelial cells of normal arteries.But the endothelial cells overlying atherosclerotic plaques as early as in the type Ⅰlesions began to express TF(3/6) with 5/8 in the type Ⅱlesions.In the type Ⅲ～Ⅵ lesions almost all endothelial cells expressed TF.TF present in all macrophages and smooth muscle cells in all stages of atherosclerosis.There was some IL-18 positive areas in the specimens(14/40,35%),TF present in all the IL-18 positive areas.Conclusion The expression of TF is closely associated with the development of atherosclerosis.

Abstract:Aim To explore the effect of niacin on cholesterol efflux in fully differentiated 3T3-L1 cells and the possible mechanism.Methods Fully differentiated 3T3-L1 cells were incubated in the medium containing various concentration of niacin(0～1.0 mmol/L) for 24 h(n=6).Reverse transcription polymerase chain reaction(RT-PCR) was used to evaluate adipocytes mRNA expression.Cholesterol efflux rate was determined through measuring release of radioactivity from 3H-cholesterol prelabled cells into medium containing apolipoprotein A_Ⅰ.Results Niacin could dose-dependently increase adenosine triphosphate binding cassette transporter A1(ABCA1) mRNA expression and apolipoprotein A_Ⅰ-mediated cholesterol efflux in 3T3-L1 cells,but had no effect on scavenger receptor class B type Ⅰ mRNA expression.Peroxisome proliferator-activated receptor gamma(PPARγ) presented high level expression in fully differentiated 3T3-L1 cells.Compared with control(0.38±0.29),niacin significantly enhanced PPARγ mRNA expression.The maximal induction was obtained with 1.0 mmol/L of niacin.Conclusion Niacin could enhance the ABCA1 pathway in adipocytes,up-regulate PPARγ mRNA expression,then increase cholesterol efflux.This new action of niacin might provide rational explaination to the mechanisms of raising high-density lipoprotein cholesterol.

Abstract:Aim To establish a hyperlipidemia animal model and to study the immunologic injury and inflammtional mechanisms in the pathogenesis of hyperlipidemia.Methods 40 BALB/C mice were randomly divided into the hyperlipidemia group and normal control group.Hyperlipidemia group were fed with high-cholesterol diet(HCD);Control group were fed with normal diet.The changes of lipid metabolism and 5 kinds of Th1/Th2 cytokines were observed in two groups.Results Interleukin-2(IL-2),IL-4,IL-5,tumour-necrosis factor-α(TNF-α) and Interferon-γ(IFN-γ)were 3.75±0.07,6.16±0.55,18.70±4.61,57.97±6.57 and 6.17±0.76 ng/L in hyperlipidemia group.Compared with control group,the levels of total cholesterol(TC),triglyceride(TG),IL-5 and TNF-α were significantly higher in hyperlipidemia group(p<0.01).But no difference was noted in the levels of IFN-γ,IL-2 and IL-4(p>0.05).And there was obviously positive correlation between IL-5 and TNF-α(r=0.79,p<0.05).Conclusion Immunologic injury and inflammtional mechanisms played an important role in the occurrence and development of hyperlipidemia and atherosclerosis.

Abstract:Aim To explore the clinic,neuroimage and cerebrovascular hemodynamics changes in patients with chronic progressive occlusion of middle cerebral artery stem.Methods Seventy five patients with middle cerebral artery stem occlusion were measured by MRI,CT and transcranial Doppler.Results Sixty five patients(86.7%)presented cerebral ischemic symptom and sign.Seventy patients(93.3%) showed different cerebral infarction in MRI or CT.The hypoperfusion watershed infarct and lacunar infarct were most common.The blood flow velocity of occlusion side middle cerebral artery sample depth remarkably reduced in 60 patients(80.0%).Seventy patients(93.3%)showed blood flow velocity compensative acceleration of the occlusion side anterior cerebral artery.Forty five patients(60.0%) showed blood flow velocity compensative acceleration of the occlusion side posterior cerebral artery.Conclusions For patients with chronic progressive occlusion of middle cerebral artery stem,transcranial Doppler and neuroimage might help to evaluate the patient' condition and prognosis,they might be important and objective index in understanding the compensative state of collateral circulation and in studying deeply the pathologic mechanism of ischemic cerebrovascular disease.

Abstract:Aim To evaluate the effects of Helicobacter pylori(Hp) eradication on coronary events and the serum levels of inflammation substances in acute coronary syndrome patiens.Methods 46 Unstable angina patiens and 110 acute myocardial infarction patiens with evidence of Hp infection were randomized into control group(n=82) and treatment group(n=74).Treatment group were treated with Omeprazole,clarithromycin and amoxicillin for ten days in addition to conventional therapy(control group) were used for Hp eradication.Results The follow up period was one year.The incidence of the coronary artery events were 6.76% in treatment group and 14.63% in control group,with a reduction of relative risk by 48.85% for treatment group(P=0.0246),Among them,incidence of non-fatal myocardial infarction reduced by 65.87% in treatment group compared to control group(5.41 %vs15.85%,P=0.0387);the incidence of the other events also decreased by 61.92% in treatment group compared to control group(14.86% vs 39.02%,P=0.005),Among them,the demand for PCI/CABG was 4.05% in treatment group and 14.63% in control group,with a reduction of relative risk by 72.35% in treatment group(P= 0.0252),readmission with angina reduced by 60.88% in treatment group compared to control group(8.11% vs 20.73%,P=0.0269).The serum levels of IgG,high sentsitivity C-reactive protein(hsCRP),soluble intercellular adhesion molecule-1(sICAM-1) and interleukin-6(IL-6) in treatment group were distinctly lower than those in control group after treatment(p<0.01 or p<0.05).Conclusion Hp eradication can significantly decrease the incidence of nonfatal myocardial infarction and readmission with angina and the demand for PCI/CABG.

Abstract:Aim to investigate the diagnostic value of 64 CT angiography in determining myocardial bridge(MB) and mural coronary artery(MCA).Methods 1 422 patients were underwent 64 CT angiography.The CTA data were post-processed and reconstructed in the work-station.All the cases with MB were submitted to CAG studies.The length,thickness of MB and the stenoses of MCA were recorded for study.The results of CT and DSA were compared and analyzed finally.Results 104 cases with 119 MB segments were found in the study,with mean length 5.0±2.7 mm,mean thickness 2.0 1.6 mm.The mean stenosis rate of MC is 31%±17%.42 cases with 44 MB segments were found by DSA study.The length and thickness were statistically deferent from CT results.Conclusion CTA can clearly characterize MB and MCA,and has important clinical values.

Abstract:Aim To investigate difference of endothelium-dependent vasodilation or arterial elasticity in patients with different grade of coronary artery pathologic change.Methods Ninety six subjects were divided into four groups by results of coronary angiography:22 cases of normal coronary artery,20 cases of early coronary artery lesions,54 cases of coronary heart disease;coronary heart disease group was divided into two groups by the Gensini scoring:19 cases of gentle coronary artery lesions(Gensini score<20),35 cases of severe coronary artery lesions(Gensini score≥20).Flow-mediated dilation in the brachial artery was evaluated by ultrasound Doppler flow method.They were also underwent a noninvasive assessment of large artery and small artery elasticity index by using pulse wave analysis.Results The flow-mediated dilation of normal coronary artery,early coronary artery lesions,gentle coronary artery lesions,severe coronary artery lesions were 9.79%±4.33%,8.21%±4.38%,5.34%±3.67% and 4.59%±2.20% respectively.The large artery elasticity index of the four groups were 917.3±315.8,870.7±266.9,849.8±346.7,795.6±134.6 μL/Pa,respectively.The small artery elasticity index of the four groups were 5.05±2.01,4.68±1.43,4.02±2.10,3.23±1.81 mL/Pa respectively.The difference among the four groups was significant on flow-mediated dilation and small artery elasticity index(p<0.05).However,there was no significant difference among the four groups on large artery elasticity index.Conclusions Following the increase of grade of coronary artery pathologic change,the impaired endothelium-dependent vasodilation and reduced small artery elasticity index were worsened.The present study suggested that the measurement of flow-mediated dilation or small artery elasticity index might be used as a novel index for the determination of the pathological change grade of coronary artery.

Abstract:Aim To study the relationship between serum bilirubin levels and coronary artery disease.Methods 120 patients undergoing diagnostic coronary arteriography(CAG) were divided into two groups:the control group(the results of CAG is normal) with 40 patients,the coronary heart disease group(≥50% diameter narrowing) with 80 patients.15 cases of coronary heart disease group were mild,32 cases of coronary heart disease group were moderate and 33 cases of coronary heart disease group were severe.The serum bilirubin was determined with Malloy-Evelyn.Results Serum Level of bilirubin in coronary heart disease group were statistical significantly compared with control group(p<0.05).There was positive correlation between the serum level of bilirubin and the degree of coronary aretry narrowing(r=-0.26,p<0.05).Conclusions Low serum level of bilirubin may be a new dangerous factor for coronary heart disease.There is correlation between serum level of bilirubin and the degree of coronary aretry narrowing.

Abstract:Aim To investigate the changes and the clinical significances of blood lipids in patients with systemic lupus erythematosus(SLE).Methods Retrospective analysis was done on the changes of blood lipids in 85 SLE patients who hadn't been treated with steroid,56 SLE patients who had been treated with steroid and 60 healthy individials.Results The level of triglyceride(TG),total cholesterol(TC) and low density lipoprotein cholesterol(LDLC) were higher in SLE patients who had been treated with steroid than that of control and that of SLE patients who hadn't been treated with steroid(p<0.05),but had no significant difference between SLE patients who hadn't been treated with steroid and control(p>0.05).The level of blood lipids was abnormal in 51.8%(44/85) of the patients who hadn't been treated with steroid,and in 22/25 patients who had renal involvement.It had significant difference between SLE patients who had renal involvement and control(p<0.01).Conclusions The levels of blood lipids were abnormal in SLE patients.It is associated with renal involvement and high dose corticosteroids.The measurement of blood lipids is important for the patient with SLE to prevent and treat the complication of the disease of high blood lipids.