Abstract:Aim To evaluate potential acylation stimulating protein (ASP) resistance in both adipocytes and preadipocytes under the conditions which produce insulin resistance by 17β-estradiol on both receptor level and post-receptor level. Methods 3T3-L1 preadipocytes were induced differentiated and 0 mol/L (17β-estradiol-free DMEM/F12), 10-8 mol/L, 10-7 mol/L and 10-6 mol/L 17β-estradiol was added to cultured 3T3-L1 adipocytes and preadipocytes overnight. RT-PCR and flow cytometry were used to detect mRNA and cell surface expression of ASP receptor. Both non-17β-estradiol treated and 17β-estradiol treated 3T3-L1 cells were cultured with 5.0 μmol/L ASP for 4 hours. Then the cell proteins were extracted and the expressions of Gβ, Gαq/11, p-PKCα and p-PKC ζ were measured by Western Blot. Results High dose 17β-estradiol suppressed C5L2 mRNA and protein expression in 3T3-L1 adipocytes but not preadipocytes. At 10-8 mol/L, 17β-estradiol increased C5L2 mRNA and protein expression slightly in both adipocytes and preadipocytes (P>0.05, respectively). At 10-6 mol/L 17β-estradiol inhibited C5L2 mRNA and cell surface C5L2 expression by 40% (P<0.05) and 21% (P<0.05), respectively. After overnight incubation with 17β-estradiol (in adipocytes and preadipocytes), Gαq/11, Gβ, p-PKCα and p-PKCζ were downregulated in the presence of ASP treatment to a certain degree. In adipocytes, at 10-6 mol/L, 17β-estradiol inhibited the ASP-induced Gαq/11, Gβ, p-PKCα and p-PKCζ expression by 17%,23%,15% and 15% (P>0.05, respectively). Whereas high dose 17β-estradiol effectively blocked ASP-stimulated Gαq/11 significantly by 24% (P<0.05) in preadipocytes. However, 17β-estradiol counteracted ASP-stimulated Gαq/11, Gβ, p-PKCα and p-PKCζ to some extent. Conclusion 17β-estradiol induces ASP resistance in adipocytes and preadipocytes. ASP resistance may contribute to the physiological abnormalities associated with insulin resistance induced by 17β-estradiol.

Abstract:Aim To explore the effect of niacin on reverse cholesterol transport in mice in vivo and the possible mechanism. Methods 14 C57BL/6 mouse were treated with ordinary diet and niacin in diet for 4 weeks, then the semice were injected intraperitoneally with 3 H-cholesterol-labeled and cholesterol-loaded raw 264.7 macrophages (0.5 mL/mice, the amount of cells achieve 5.0×106). After 24 h, feces were collected and analyzed the appearance of 3 H-tracer (as the percentage of the total injected counts). Reverse transcription polymerase chain reaction (RT-PCR) were used to evaluate ABCG5; CYP7α, LXRα mRNA of liver and ABCG5, LXRα mRNA of intestina expression. And the level of ABCG5, LXRα proteins of liver and intestina was analyzed by Western blot. Results It was observed that niacin increased reverse cholesterol transport by 21% in mice in vivo in contrast to the control (P<0.05). Niacin increated ABCG5; CYP7α, LXRα mRNA of liver and ABCG5, LXRα mRNA of intestina expression, while having the same effect on the expression of liver, and intestina, ABCG5 and LXRα protein. Conclusion These results suppose that niacin promtes reverse cholesterol transport by increasing ABCG5 and CYP7αexpression which is stimulated by LXRα.

Abstract:Aim To observe the effect of Krppel-like factor 4 (KLF4) overexpression on heat stress-induced apoptosis of C2C12 cells. Methods C2C12 cells transfected with pcDNA3.1 or pcDNA3.1-KLF4 plasmids were exposed to heat stress (42.5℃) for 1 h and recovered at 37℃ for 12 h. Flow cytometry (FCM) and Hoechst33258 staining assays were performed to assess the apoptosis; the expression of Bcl-2 and Bax was detected by Western blots. Results After heat stress, FCM showed that apoptotic cells significantly increased and the percent of apoptosis was 19.6% in the cells overexpressed with KLF4. It was determined were characterized with classical morphologic changes including apoptotic body and nuclear condensation in the cells overexpressed with KLF4.And the expression of Bcl-2 and Bax also significantly changed in the cells overexpressed with KLF4. Conclusion KLF4 overexpression promoted the apoptosis of C2C12 cells.

Abstract:Aim To investigate the effect of culture of vascular smooth muscle cell (VSMC)in vitro on its phenotype transformation and to survey the best parameters for characterizing the phenotype. Methods The primary VSMC and the fourth passage of VSMC in vitro culture were compared by their morphological characteristics under electron microscope, and the α-SM actin expression measured by immunohistochemical staining, the osteopontin (OPN), SM22α, matrix Gla protein (MGP) mRNA transcription levels measured by RT-PCR were all comparatively studied. Results The myofilament was abundant, the dense patch—a typical structure of contractile phenotype of VSMC well-distributed, while the endoplasmic reticulum, Golgi's complex were less-distributed in primary VSMC. The VSMC in fourth passage, however, showed scattered distribution of myofilament in cytosol, but much more endoplasmic reticulum, Golgi's complex, and mitochondria under electron microscope observation. The immunohistochemical staining showed much strongerα-SM actin expression in primary VSMC compared with the fourth passage (P<0.05). OPN mRNA level increased very significantly (P<0.01) in fourth passage of VSMC compared with primary ones. The transcription level of SM22α mRNA in fourth passage of VSMC decreased compared with primary VSMC, but the difference was not significant (P>0.05). MGP mRNA transcription levels increased in fourth passage of VSMC (P<0.05). Conclusions Four passages of VSMC in vitro can transform the typical contractile phenotype into the typical synthetic phenotype, which indicated that the VSMC had the trait of easy-dedifferentiation, this may suggest some implication in VSMC research. Electron microscope observation, α-SM actin, OPN showed significant difference between contractile phenotype and synthetic phenotype, which may represent a good marker for distinguishing the two different phenotypes. The SM22α and the matrix Gla had some difference, but overlaps were also significant between the two different phenotypes.

Abstract:Aim To study the effects of simvastatin on L-type calcium current and Cav1.2 protein levels in atrial myocytes of rats with diastolic heart failure. Methods 30 SD rats were randomly divided into 3 groups: control group, DHF group, Simvastatin group. DHF model was produced by abdominal aortic coarctation in the rats in the operating group. The rats in Simvastatin group were given with Simvastatin 2 mg/(kg·d) by intragastric administration for 4 weeks, the others were treated with equal isotonic Na chloride. After 4 weeks, hemorheology was assessed by catheterization. Single atrial myocytes were isolated by enzymatic dissociation and L-type calcium current were investigated using a whole-cell patch-clamp technique. The expression levels of ICa-L alpha-subunit (Cav1.2) in atrial myocytes were measured by Western-blot analysis. Results (1) Compared with control group, DHF rats exhibited increases in LVSP, LVEDP and Tau. They also showed a decrease in -dp/dtmax. Compared with DHF group, LVSP, LVEDP and Tau were decreased and -dp/dtmax was increased in Simvastatin group. There were no differences in heart rate, the maximum ascendsus ratio for +dp/dtmax and Cm among three groups. (2) Compared with control group, the peak densities of atrial ICa-L in DHF group were decreased, while activation curves, inactivation curves and recovery curves were not altered. Compared with the DHF group, Simvastatin increased the peak density of atrial ICa-L and made the inactivation curves shift to more positive potentials in DHF rats, but did not change activation curves and recovery curves. (3) Campared with control group, DHF rats exhibited decreases in the protein levels of Cav1.2 in atrial myocytes. Simvastatin increased Cav1.2 protein levels in atrial myocytes in DHF rats. Conclusion Simvastatin increases the peak density of atrial ICa-L in DHF rats and the effects are related with the increases in Cav1.2 protein levels.

Abstract:Aim To investigate the apoptosis and mitochondrial membrane potential (MMP) of nerve cell after cerebral ischemia-reperfusion for 2 hours and intervention of extraction from Piper Kadsura Ohwi. Methods The models of middle cerebral artery occlusion (MCAO) and reperfusion were set up. The apoptosis and MMP of nerve cell were detected among sham operated group, ischemic group, DMSO-treated (dimethyl sulfoxide) group and extraction from Piper Kadsura Ohwi-treated group in the cerebral tissue around infarction 2 hours after ischemia-reperfusion separately through flow cytometry. Results 2 hours after ischemia-reperfusion, the apoptosis rate of nerve cell of Piper Kadsura Ohwi-treated group (47.0%±3.8%) decreased obviously accompanied with obvious increase of MMP (0.182±0.023) compared with ischemic group (P<0.01). The apoptosis rate of nerve cell (5.8%±1.8%) and MMP (0.331±0.016) of sham operated group were different from those of ischemic group. Conclusion Extraction from Piper Kadsura Ohwi plays a significant role in protecting cerebral tissue through decreasing the apoptosis and stopping decrease of MMP of nerve cell caused by ischemia-reperfusion.

Abstract:Aim To investigate the effects of Smad3 on the growth of C2C12 cells induced by transforming growth factor-β1 (TGF-β1). Methods C2C12 cells were transfected with siRNA-Smad3 in vitro, and the cell proliferations were examined with MTT methods. Results After the C2C12 cells interfered with different concentrations of TGF-β1 (0, 1, 5, 10 μg/L) for 24 h, the MTT (OD) values were 0.096±0.015, 0.177±0.014, 0.240±0.028, 0.312±0.012 respectively (P<0.01). While the C2C12 cells were transfected with 200 pmol/L siRNA-Smad3 for 24 h and then treated with 5 μg/L of TGF-β1, the MTT(OD) values in experimental group (0.063±0.011) were decreased compared with internal control group (0.137±0.016, P<0.01). Conclusion The TGF-β1 could promote C2C12 cells proliferation by the Smad3 pathway and showed dose dependent manner, siRNA-Smad3 could block the signal transduction and decreased C2C12 cell proliferation effectively.

Abstract:Aim To investigate the effect of advanced glycation end products (AGE) on number of endothelial progenitor cells (EPC). Methods AGE was prepared by incubating D-glucose and human serum albumin (HSA) for 90 days, and then identified by fluorescence and SDS-PAGE.The mononuclear cells (MNCs) were isolated from peripheral blood of healthy adults,then were plated on fibronectin-coated culture 6-well plates respectively in EC basal medium-2.After cultured 7 days, attached cells were identified with antibodies recognizing human CD34,CD133 and KDR, several groups of attached cells plated in the absence or presence of AGE (2 mg/L, 20 mg/L, and 200 mg/L) for 0 h, 24 h, 48 h, and 72 h respectively,the cells were quantified by viewing 10 random microscopic fields. Results AGE showed the absorption and fluorescent spectra. After cultured 7 days, attached cells were identified with antibodies, the results demonstrated the positive expression of antibodies were KDR (78.60%±2.20%), CD34 (8.60%±2.00%) and CD133 (2.80%±0.60%). The decrease number of EPC per field was observed under 200 mg/L AGE at 24 h (146.67±4.98), while reached to the minimum (73.67±3.76) at 72 h. However, no statistical change in cell number was identified when cells were treated under low density AGE (2 mg/L and 20 mg/L). Conclusion High AGE concentration may inhibit EPC augmentation.

Abstract:Aim To investigate the effect of chitosan on the endothelial regeneration and neointimal proliferation after balloon angioplasty of right carotid artery in rat. Methods Endothelial denudation of artery in rat were performed with 2F balloon catheter. 50 SD rats were randomly divided into the modle group and the chitosan group. Artery were harvested on 0 d, 7 d, 14 d, 28 d and 35 d after the injury, respectively. The reendothelialization and the neointimal hyperplasia were observed cross-sections stained with hematoxylin-eosin from the injured segments processed for histological and morphological study by computer-assisted picture analysis system. Results Chitosan enhanced the reendothelialization of the injured arterial determined vascular endothelial growth factor (VEGF) staining. The absolute reendothelialization was observed on 14 d in chitosan group but on 28 d in the modle group. Intimal hyperplasia and lumen stenosis can be seen on the 7 d after injury. At the 28th day the intimal thicken reached at a peak. In the chitosan group the intimal thicken reached at a peak at the 14 th day. There were significant difference of neointimal in the chitosan group and the modle group on the 14th day, 28th day and 35th day. Conclusions Chitosan is effective on intimal hyperplasia and lumen stenosis after arterial injury by inhibiting neointimal proliferation which is related to the reendothelialiazation.

Abstract:Aim To investigate the anti-atherosclerosis mechanism of phlegm-clearing treatment, Gualou Xiebai Banxia Decocation(GXBD), a typical decoction of phlegm-clearing treatment was studied in the effect of modulating the metablism of proteoglycans in aorta. Methods Thirty rabbits were randomly divided into three groups, normal group, model group, and treatment group. Model group and treatment group were fed with high lipid food to induce the pathological change of atherosclerosis, besides, treatment group was given with GXBD everyday, while normal group was fed with normal food as control. After six weeks, blood fat and aorta atherosclerosis pathological changes was examined, proteoglycans was extracted and turned to glycosaminoglycan by enzymolysis, electrophoretic analysis was used to determine the consist and content of every kinds of glycosaminoglycan. Results The atherosclerosis pathological changes was evident, in conrtol group, the contents of cholesterin and low density lipoprotein were 13.43±3.12 mmol/L and 8.53±1.37 mmol/L, the contents of chondroitin and dermatan sulfate were 21.93±1.82 mg/g, 15.56±1.61 mg/g; whereras in model group, cholesterin and low density lipoprotein increased to 23.63±4.31 mmol/L, 15.63±1.27 mmol/L (P<0.01), chondroitin and dermatan sulfate increased to 31.23±1.41 mg/g, 19.36±1.64 mg/g (P<0.01 or P<0.05); contrast to model group, the atherosclerosis pathological changes in treatment group were evidently imporved, the contents of chondroitin and dermatan sulfate decreased to 22.33±1.58 mg/g, 14.36±1.71 mg/g(P<0.01 or P<0.05), the content of cholesterin, low density lipoprotein of blood shows little changes. Conclusions The gualou xiebai banxia decocation exerts the anti-atherosclerosis effect by modulating the metablism of proteoglycans rather than modulating the blood fat.

Abstract:Aim To investigate the relationship between glycosylated haemoglobin (HbA1c) levels and intima-media thickness of the common carotid artery (CCA-IMT) and the predicting effects of HbA1c on CCA-IMT in newly diagnosed type 2 diabetes (T2DM). Methods The CCA-IMT was assessed using non-invasive high resolution B-mode ultrasonography. Age, sex, metabolic parameters including body mass index (BMI), fasting plasma glucose (FPG), HbAlc, serum lipids, blood pressure, 24 h albuminuria (UALB) and CCA-IMT were compared among T2DM without subclinical atherosclerosis (As) (T2DM group), T2DM with subclinical atherosclerosis (As group) and T2DM with clinical atherosclerosis (CHD group). The 156 newly diagnosed T2DM (duration≤1 year) without atherosclerosis received the multifactorial targeted intervention, including taking aspirin and controlling blood glucose, blood pressure, blood lipid and body weight. The differences of metabolic control were analyzed between CCA-IMT progressive group and non-progressive group. Logistic regression analysis was used to disclose the correlation between the CCA-IMT and macrovascular risk factors. Results HbAlc levels were higher in CHD group than those in T2DM group and atherosclerosis group (P<0.01). CCA-IMT had a linear correlation with HbAlc in T2DM patients(r=0.106, P=0.049). After multifactorial intervention for 2 years, △CCA-IMT had a linear correlation with baseline HbA1c (r=-0.189, P=0.024)and △HbA1c(r=0.166, P=0.041). △HbA1c was lower in the CCA-IMT progressive group than those in the CCA-IMT non-progressive group. Logistic regression analysis showed that △HbA1c was closely correlated with CCA-IMT. Conclusions HbAlc level was one of risk factors for CCA-IMT in T2DM. Under the multifactorial intervention for 2 years, baseline HbA1c and △HbAlc may predict the progression of CCA-IMT in patients with newly diagnosed type 2 diabetes.

Abstract:Aim To investigate malondialdehyde-modified (MDA) low-density lipoprotein (LDL), its immune complexes (LDL-IC) and blood lipid levels in patients with rheumatoid arthritis (RA), and to explore the mechanism of high incidence from cardiovascular disease in patients with RA. Methods 55 patients with RA, 13 of which had cardiovascular disease (CAD), 42 of which had simply RA, and 60 healthy controls were randomly chosen. Plasma MDA-LDL and its immune complexes (LDL-IC) levels were determined by enzyme linked immunosorbent assay (ELISA). Blood lipid levels and inflammatory markers were also studied. All data was subjected to statistical analysis. Results Compared with the control, plasma lipids and apolipoproteins levels in patients with RA remained unchanged, except that apolipoprotein B levels increased in RA patients (P<0.05); meanwhile no difference was found in plasma lipids and apolipoproteins concentrations between simple RA patients and RA patients with CAD. MDA-LDL level in RA with CAD, simple RA and control were 187.81±90.89 mg/L, 102.01±57.73 mg/L and 32.65±27.00 mg/L, respectively. LDL-IC level in the three groups were 2.58±1.69 AU, 1.87±0.74 AU and 1.21±0.38 AU, respectively. Compared with the control, MDA-LDL and LDL-IC levels increased in simple RA patients and RA patients with CAD (P<0.01), furthermore, MDA-LDL and LDL-IC levels in RA patients with CAD were both found significantly higher than those in simple RA (P<0.01 or 0.05). LDL-IC level was found related with C-reactive protein (r=0.301, P=0.026), and MDA-LDL level tended to be related with erythrocyte sedimentation rate (r=0.263, P=0.057). Conclusions MDA-LDL and LDL-IC were both increased in RA patients, and may play an important role in atherocslerosis.

Abstract:Aim To study the effect of G4A polymorphism on serum lipid level of Han population in Hunan province. Methods G4A Polymorphisms of the scavenger receptor class B type I (SR-BI) gene were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in atherosclerotic cerebral infarction (ACI) patients, cerebral hemorrhage (CH) patients and healthy controls. Results The serum level of high density lipoprotein cholesterol (HDLC) in SR-BI G4A GA+AA genotype subgroup was markedly higher than that in GG genotype subgroup of the same group in ACI group (P<0.05), and it was the same with CH group. The serum level of low density lipoprotein cholesterol (LDLC) in SR-BI G4A GA+AA genotype subgroup was evidently lower than that in GG genotype subgroup of the same group in ACI group (P<0.05), and it was the same with CH group. The serum level of HDLC in SR-BI G4A GA+AA genotype subgroup was obviously higher than that in GG genotype subgroup of the same group in control group (P<0.05). The serum levels of LDLC and total cholesterol (TC) in SR-BI G4A GA+AA genotype subgroup was significantly higher than those in GG genotype subgroup of the same group in control group (P<0.05). The serum level of HDLC in male ACI group and GA+AA genotype subgroup of CH group was markedly higher while LDLC was markedly lower than that in GG genotype subgroup of the same group. There was no significant difference of body mass indexes and serum level in different genotype subgroups of female ACI group and CH group. Conclusions A allele of SR-BI G4A is possibly associated with the metabolism of HDL and LDL.

Abstract:Aim To assess the effect of postprandial triglyceride and serum level of NO,ET-1 on the endothelial function in elderly type 2 diabetic patients. Methods Subjects were assigned to two groups: the observed group of 26 fasting normotriglyceridemic elderly type 2 diabetic patients who were divided into two subgroups: subgroup 1[4 hour postprandial blood triglyceride level (TG≤2.0 mmol/l)] and subgroup 2 (TG>2.0 mmol/l)according to the level of TG±2S in control group; the healthy control group of 10 normal subjects. Total triglyceride(TG)、NO、ET-1 were measured before and 2, 4 and 6 hours after a fatty meal (600 kcal, 56%fat, 6.5%protein, 37.5%carbohydrate). Carotid IMTc、fasting FMD and 4h after the meal were measured by ultrasonography. Results 2, 4, 6 hours after a fatty meal, there was a slightly postprandial rise of TG and significant differences in TG4h between the observed group and healthy controls(P<0.05). NO/ET-1 was positively correlated with FMD and IMTc. Conclusion There are postprandial triglyceride metabolic disturbance in the elderly diabetes, which leads to compensate increasing production of NO and moment descendent of ET-1 level. The disturbance of the equilibrium between TG and NO, ET-1 which induced endothelial dysfunction may be correlated with diabetic angioplasty.

Abstract:Aim To determine endothelial function and chemokine activity of peripheral blood monocytes in individuals with primary hypertension, and to explore the role of asymmetric dimethylarginine in this process. Methods 42 patients with essential hypertension and 40 healthy controls in the out-patient clinic and admission department of the hospital were enrolled in the present study. Flow-mediated dilation on brachial artery was assessed and the plasma levels of asymmetric dimethylarginine and monocyte chemoattractant protein-1, and the expression of CCR2 protein expression in peripheral blood mononuclear cells were also determined. Results Endothelial dysfunction were found in patients with primary hypertension, manifesting that flow-mediated dilation was decreased, the levels of asymmetric dimethylarginine were elevated, and the levels of nitric oxide were decreased (P<0.05). Compared with controls, plasma levels of monocyte chemoattractant protein-1 were markedly elevated and the expression of CCR2 protein in separated peripheral blood mononuclear cells in hypertensive patients was significantly upregulated (P<0.05). Incubation of mononuclear cells with exogenous asymmetric dimethylarginine (30 μM) for 24 h further upregulated the expression of CCR2 protein in hypertensive patients (P<0.05). Conclusions Asymmetric dimethylarginine may promote endothelial dysfunction and activation of monocytes existed in patients with essential hypertension.

Abstract:Aim To investigate the plasma concentration of amino-terminal pro-brain natriuretic peptide (NT-proBNP) and prognosis impact in patients suffering from of cerebrovascular accident. Methods We selected 137 patients suffering from acute cerebrovascular accident in our department, from August 2005 to April 2006; and 106 healthy people, who came for body-check, as control. All the people received blood test for NT-proBNP, and all patients received echocardiogram examination for left ventricular ejection fraction (LVEF), Glasgow coma score (GCS) and Glasgow outcome score (GOS). According to the NT-proBNP result, the patients were divided into two groups:the high concentration group and the low concentration group. We compared the NT-proBNP concentration between the cases and control, the incidence of heart failure between different concentration groups of NT-proBNP;analyzed the linear correlation relationship between the concentration of NT-proBNP and GCS and GOS. Results The plasma concentrations of NT-proBNP in cerebral infarction group, cerebral hemorrhage group, and subarachnoid hemorrhage group were higher than in control (P<0.01), further more, the subarachnoid hemorrhage group showed highest level than the other groups (P<0.05). The high NT-proBNP concentration group showed the same incidence of heart failure as low group (P>0.05). There were negative linear correlation relationship between the plasma concentration of NT-proBNP and GCS (r=-0.696, P=0.039), GOS (r=-0.726, P=0.008). Conclusions cerebrovascular accident can independently cause the raise of plasma concentration of NT-proBNP, the degree of that can perhaps reflect the severity and prognosis of disease.

Abstract:Aim To explore functional status of the main arteries of cerebral anterior and posterior circulation system in elderly patients with vascular vertigo, clinical images of cerebral blood flow features were detected and carotid atherosclerosis or plaque were observed. Methods The color doppler flow sonographic features (Siemens Acuson Sequoia 512 ) and transcranial Dopper (TCD) indexes (MT-3000) of carotid arteries in 185 patients with vascular vertigo were observed and evaluated prospectively. Results Simple function change of cerebral anterior or posterior circulation system were found in 14 cases(7.57%) and 42 cases (22.7%) vertigo patients. There was significant difference between the two groups (χ2 =7.67, P<0.01). Both function change of cerebral anterior and posterior circulation system were found in 105 cases (56.8%) vertigo patients. Cerebral atherosclerosis or carotid vessels elasticity decrease is common in 137 cases (74.1%). Carotid atherosclerosis plaques was found in 97 cases (52.4%). The plague was mostly located at the bifureation of the carotid artery, only 3 cases at the vertebrobasilar arterial system. Conclusion Among patients, vascular vertigo occurrence is related not only with the function abnormalities of posterior circulation, but also with the anterior part, even only manifesting with the simple anterior circulation lesion. Atherosclerosis or plaque form is also the most important factor for cerebral circulation insufficiency.

Abstract:Aim To evaluate the effect and diagnosis value on oxidized low density lipoprotein (ox-LDL) in the developing process of acute cerebral ischemia. Methods Eighty acute cerebral ischemia patients were chosen during June to August, 2007. These patients were divided two groups: the acute cerebral infarction (ACI) group and transient ischemic attack (TIA) group, based on the functional MR examination. The serum ox-LDL antibody was tested by enzyme-linked immunosorbent assay (ELISA) method, the main serum biochemistry indexes were detected to all patients and statistics analysis was performed. Results The patients in ACI group had a 38.07±32.14 ku/L ox-LDL antibody level and in TIA group had a 25.18±9.17 ku/L ox-LDL antibody level. A significant difference as compared with the patients in the twe group (P<0.05). The other biochemics index had no dependability in the two groups. Conclusions The patients with The ox-LDL antibody level is distinct in different type of cerebral ischemia patients and a higher level may give a danger signal with acute cerebra infarction.

Abstract:Aim To analyse the cost and effect of nifedipine, captopril or combined treatment used as anti-essential hypertension therapy programs. Methods This study was a randomised single blind controlled clinical trial conducted in outpatient. The trial randomised 330 patients with mild to moderate hypertension aged 18～70 years were divided into three treatment groups: captopril (50 mg daily) group, nifedipine (40 mg daily) group, and captopril+nifedipine group for a mean follow up of 8 week. Results The effects of captopril group and nifedipine group after 8 week administration had similar result. But there was significant difference in blood pressure between captopril+nifedipine group and captopril or nifedipine group. Conclusion Nifedipine combined with captopril lowers blood pressure sufficiently to achieve therapeutic goal in most patients with hypertension.