Abstract:AimTo investigate whether Rho kinase inhibitor fasudil inhibits high glucose (HG)-induced cell adhesion to human umbilical vein endothelial cells (HUVEC) and search for its underlying mechanisms.MethodsThe adhesion of monocytes to HUVEC was determined using fluorescence-labeled monocytes.The mRNA and protein levels of vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemotactic protein 1（MCP-1） were measured using real-time polymerase chain reaction (RT-PCR) and Western blot.The amounts of RhoA, ROCK1, p-MYPT and MYPT were determined using Western blot analysis.ResultsFasudil significantly suppressed HG-induced adhesion of THP-1 to HUVEC in a dose manner: namely by about 33.4% in the presence of low dose (10-6 mmol/L) and by 42.8% in high dose (10-5 mmol/L) at 24 hours (both P<0.05); the same for 12 h.Fasudil significantly suppressed the HG-induced increase of mRNA and protein expression of VCAM-1 and MCP-1 in dose and time manner.HG markedly increased ratio of p-MYPT/MYPT, while fasudil inhibited HG-induced activation of Rho/ROCK pathway.Conclusion The adhesion of monocytes to HUVEC and the expression of VCAM-1and MCP-1 induced by high glucose could be inhibited by fasudil，the same to activation of Rho/ROCK pathway.It suggests that fasudil may represent a new treatment for diabetic vascular injury.

Abstract:AimTo study the effect of the concentration of calcium chloride on the establishment of abdominal aortic aneurysm model, and provide basis for the research on abdominal aortic aneurysm.MethodsThirty male New Zealand white rabbits were chosen and randomly divided into three groups, including experimental group one, experimental group two and control group.Their abdominal aorta were respectively underwent infiltration of 0.5 mol/L calcium chloride, 0.75 mol/L calcium chloride, and physiological saline.Six weeks later, the diameter of the aorta was measured, and the aortic tissue was exercised for histological examination.ResultsDilatation rate of abdominal aorta of experimental group one, experimental group two and control group was 87.31%, 124.12% and 12.10% respectively.Compared with experimental group one, experimental group two had more significant destruction and degradation of elastic fiber, decrease of smooth muscle cell, and dilation of abdominal aorta.ConclusionInfiltration method with 0.75 mol/L calcium chloride is a simple, feasible method to establish rabbit abdominal aortic aneurysm model.

Abstract:AimTo investigate the influence of glucose and irbesartan on expression of lectin-like oxidized low density lipoprotein receptor-1（LOX-1） mRNA and protein in cultured THP-1 cells. MethodsTHP-1 cells were differentiated by incubation with 160 nmol/L PMA for 72 hours.The cells were divided into three groups: control group, glucose group (5 mmol/L, 8 mmol/L, 12 mmol/L, 15 mmol/L), irbesartan intervention group.Then the cells in glucose group were incubated with several concentrations glucose for 24 hours. The intervention group was first treated with irbesartan for 2 h, then co-incubated with 15 mmol/L glucose for 24 h.Real time PCR and enzyme-linked immunosorbent assay technology were used to detect LOX-1 mRNA and protein expression.ResultsCompared with control group,the expression of LOX-1 mRNA and protein showed no significance in 5 mmol/L glucose group(P>0.05),but the expression in high glucose groups increased significantly (P<0.05),and the increase was dependent on high glucose concentration.Irbesartan group markedly decreased high glucose-induced LOX-1 mRNA and protein expression.ConclusionHigh glucose can upregulate LOX-1 mRNA and protein expression in a concentration-dependent manner and may contribute to the pathogenesis of atherosclerosis.Irbesartan can block the effect of high glucose and may produce anti-atherosclerosis.

Abstract:AimTo observe the effects of amlodipine only or with the enalapril on aorta remodeling and the activities of Na+ pump and Ca2+ pump, and the mRNA expressions of α1 subunit of Na+ pump and plasma membrane calcium pump isoform 1(PMCA1), and investigate the possible mechanisms of aorta remodeling attenuation by amlodipine and enalapril.MethodsRenovascular hypertensive rat model was induced by two-kidney-one-clip method, and rats were consecutively treated with normal saline(model group), amlodipine ［5 mg/(kg·d)］, enalapril ［10 mg/(kg·d)］ and amlodipine +enalapril ［2.5 mg/(kg·d)+ 5 mg/(kg·d)］ for 6 weeks（n=6).Sham -operated rats were treated with normal saline as controls(n=6).Aortic morphology and structural changes in the aortic media，the ratio of media thick-ness/ luminal diameter (MT/ LD), and media area/ luminal area (MA/LA) were observed by HE staining, immunohistochemical staining and Masson staining.The content of angtension Ⅱ(AngⅡ) was measured by radioimmunoassay, the activity of Na+ pump and Ca2+ pump in aortic media were determined by enzyme colorimetry, and the mRNA expressions of α1 subunit of Na+ pump and PMCA1 were detected by Real-time PCR, respectively.ResultsIn the model group, the ratio MT/ LD and MA/ LA of aorta and the AngⅡ content were significantly increased while the activities and the mRNA levels of Na+ pump and Ca2+ pump were obviously decreased（p<0.05～p<0.01）.In the amlodipine group and the enalapril group, the blood pressure ，the ratio MT/ LD and MA/ LA and the AngⅡ content were obviously decreased while the Na+ pump and Ca2+ pump activities were obviously increased(all p<0.01).In the amlodipine group, the mRNA level of PMCA1 was significantly increased.But in the enalapril group, the mRNA levels of both sodium pump a1-subunit and PMCA1 were obviously increased(all p<0.01).In combination group, the amelioration of the ratio MT/ LD and MA/ LA，ion pump activity and mRNA levels of PMCA1 were significantly greater than in single-drug intervened group (all p<0.01), and the AngⅡcontent also was decreased more greater than in single-drug intervened group (all p<0.01), and there were synergistic effects.ConclusionsThe combination of amlodipine and enalapril is better than single-drug therapy in the attenuation of aorta remodeling, and there may be some synergistic effects in inhibiting tissue RAS and increasing the activities of ion pumps.Ca2+ pump activity stimulated by amlodipine may be partially mediated through up-regulating PMCA1 mRNA.

Abstract:AimTo observe the regulatory effect of RhoA/Rho kinase in expression of tissure factor(TF) protein influenced by fluvastatin, so as to explore the noval target point of fluvastatin in anti-artherosclerosis and anti-thrombosis.MethodsThe same human umbilical vein endothelial cells (HUVEC) was cultured and passage cultured, and was then divided into control group, TNF-α group, Flu group, and TNF-α+Flu group, TNF-α+AngⅡ group, AngⅡ group and TNF-α+Y27632 group.RT-PCR method was used to examine the expression of TF mRNA.Western blot method was used to examine the TF protein expression and RhoA activity. TF protein expression of HUVECs in control group, TNF-α+AngⅡ group, AngⅡ group, TNF-α group and TNF-α+Y27632 group was examined using Western blot method. ResultsIn the meanwhile of inducing the HUVECS TF expression, TNF-α also activated RhoA.Fluvastatin inhibited the activation of RhoA.AngⅡ could enhance the TF expression in HUVECS which induced by TNF-α, while Y27632 could inhibit the TF expression in HUVECS which induced by TNF-α.ConclusionsThe inhibitive effect of fluvastatin on the TF expression might be achieved through the RhoA/ Rho signal regulatory pathway at small G protein level.

Abstract:AimTo investigate the effects of peroxisome proliferator-activated receptor-γ(PPARγ) on cholesterol accumulation and CD36 expression in THP-1 macrophages.MethodsMononuclear cells were induced to differentiate into THP-1 macrophages by phorbol myristate acetate (160 nmol/L) co-incubation for 24 h.THP-1 macrophages were co-incubated with 50 mg/L oxidize low-density lipoprotein (ox-LDL) and PPARγ agonist Ciglitazone（10 μmol/L;C）and PPARγ antagonist GW9662（10 μmol/L;D）for 24 h, and the cells mixed with (B) and without (A) ox-LDL were as the control groups.Cellular total cholesterol was determined by high performance liquid chromatography analysis.CD36 mRNA and protein levels were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot respectively.ResultsHigh performance liquid chromatography analysis demonstrated the amount of cellular total cholesterol were 76.28±10.36(A), 121.63±13.32(B), 136.23±14.78(C), 98.52±11.45(D) mg per gram protein.PPARγ antagonist GW9662 inhibited CD36 mRNA and protein synthesis.Ciglitazone increased CD36 mRNA and protein synthesis.The ratios of CD36/GAPDH were 0.78(A), 0.94(B), 1.12(C), 0.52(D) respectively.Western blot re-sults conform to RT-PCR outcomes. ConclusionAntagonist of PPARγ may decrease cholesterol accumulation and downregulate ox-LDL-induced CD36 expression in THP-1 macrophages.

Abstract:AimTo investigate the protective effects and mechanism of N-acetylcysteine (NAC) on myocardial injury in diabetic rats.MethodsThe diabetic model was established with streptozotocin (STZ).The fifty male SD rats were randomly divided into five groups: the normal group, the diabetic group, the low, middle and high dose N-acetylcysteine treatment group.N-acetylcysteine of 50, 100 and 200 mg/kg was respectively given to the low, middle and high dose drug treatment groups by gavage 12 weeks.Rats’ heart function, body weight (BW), heart weight (HW) and left heart weight (LHW) were tested, HW/BW and LHW/BW were calculated, the activities of SOD and GSH-PX in serum and myocardial tissue were measured.Morphological change was observed and myocardial collagen volume fraction was measured by Masson staining.The protein expression levels of TGF-β1, SMAD3 and SMAD7 were determined by immunohistochemistry.ResultsCompared with the control group, LVEDP of the diabetic group was significantly increased (P<0.01) and LVSP, ±dp/dtmax were significantly reduced (P<0.01).All the cardiac mass index and left ventricular mass index were higher (P<0.01).The activities of SOD and GSH-PX were significantly reduced (P<0.01).The levels of TGF-β1 and SMAD3 were significantly up-regulated (P<0.01), the level of SMAD7 was declined significantly (P<0.01).Compared with the diabetic group, all above indexes of NAC treatment groups had been improved by gavage 12 weeks, high dose group had been improved obviously (P<0.01).ConclusionsNAC has a protective effect on myocardial in diabetic rats, the mechanism may be related to the inhibition of oxidative stress, reducing the expression of TGF-β1, SMAD3 and increasing the expression of SMAD7.

Abstract:AimTo observe whether rosuvastatin postconditioning (RPO) and ischemic postconditioning (IPO) could attenuate ischemia-reperfusion injury (IRI) in T2DM rats, and to investigate the potential cardioprotective mechanisms involved.MethodsInduced by streptozotocin plus nicotinamide, a T2DM rat model was successfully created in 54 healthy Wistar male rats, which were randomly allocated into seven groups (n=9): Sham group, IRI group, RPO+IPO group, 5-HD group, diazoxide group, HMR-1098 group, and Cromakalim group.Infarct size, ultrastructure, serum cTnT were determined at the end of ischemia-reperfusion, which underwent 45 min ischemia and 120 min reperfusion.ResultsCompared with IRI group, the myocardial infarct size significantly decreased in RPO+IPO group and diazoxide group, HMR-1098 group, Cromakalim group (p<0.05).The myocardial infarct size in 5-HD group significantly increased than that in RPO group and diazoxide group, HMR-1098 group, and Cromakalim group (p<0.05).TEM revealed that the myocardial cell mitochondria ultrastmctural damages were serious in IRI group and 5-HD group.In RPO+IPO group, diazoxide group, HMR-1098 group and Cromakalim group, the structure of most mitochondria maintained as originally on the whole.As compared with IRI group, the level of cTnT in RPO+IPO group and diazoxide group, HMR-1098 group, Cromakalim group was significantly reduced (p<0.05).The level of cTnT in 5-HD group was significantly increased than that in RPO+IPO group and diazoxide group, HMR-1098 group, Cromakalim group (p<0.05).ConclusionsRPO+IPO could significantly attenuate IRI in vivo T2DM rats.mitoKATP channel but not sarcKATP channel plays the major role in the protection effects of rosuvastatin postconditioning.

Abstract:AimTo observe the effects of atorvastatin on NF-κB and its related inflammatory factors of ICAM-1, VCAM-1, MCP-1, IL-6, TNF-α in balloon injury model of common carotid artery in rats.MethodsIn the experiment, a total of 72 male healthy Wistar rats between 300 and 350 gram were randomly divided into three groups.They were as the followings: ① blank control group which were operated without balloon injury or atorvastatin; ②placebo control group which were operated and injured by balloon without atorvastatin; ③experimental group which were operated and injured by balloon with atorvastatin.In the experiment, the experimental group were given atorvastatin by 10mg/（kg·d) while the two control groups were given the same amount of water instead.After 3 days, all the rats were operated.However, blank control group were operated only without balloon injury.In each group, 6 of them were killed at 1, 3, 7, 14 days after operation and their blood samples and carotid arteryspecimens were taken respectively.Intimal hyperplasia was detected by histomorphology.NF-κB and its downstream factors were detected by immunohistochemistry.ResultsThe hyperplasia area of intima after balloon injury in the experiment group was significantly smaller than the placebo control group (P<0.01);The expression of NF-κB and its related factors in vascular wall and serum of the placebo control

Abstract:AimTo investigate the value of myeloid-related protein (MRP) in the early diagnosis of acute coronary syndromes (ACS).Methods81 patients were sent to the hospital within three hours of acute chest pain in this study.These patients had undergone coronary angiography.According to final clinical diagnosis, patients were designated as ACS group and non-ischemic chest pain (NICP) group.Blood samples were detected for MRP in the chest pain 0-3 h, 3-6 h, 6-12 h time periods.Compare different time, different ways of MRP sampling variation to evaluate the value of MRP in the clinical diagnosis of ACS.ResultsMRP difference of ACS group had statistical significance compared with NICP group in the chest pain 0-3 h, 3-6 h and 6-12 h time periods (p<0.05).13 cases of ACS coronary artery MRP levels difference were statistically significant compared with venous blood MRP levels (p<0.05).Receiver operating characteristic curve (ROC curve) analysis was used to determine the cut-off value of this assay for identifying individuals with ACS from NICP, sensitivity and specificity at the optimal cut-off value of 8.5 mg/L were 60.7% and 95.0%.The area under ROC curve was 0.898 (95%CI 0.815-0.981).ConclusionsMRP had high sensitivity in the diagnosis of ACS.MRP can be regarded as a early diagnosis biochemical marker of ACS.

Abstract:AimTo investigate the relationship between ambulatory blood pressure parameters and arterial stiffness in prehypertensive patients.MethodsAccording to their blood pressure level, 204 individuals were divided into three groups: namely normotensive controls (n=63), prehypertensive participants (n=74) and hypertensive patients (n=67).The 24 h ambulatory blood pressure monitoring was used to record 24 h systolic blood pressure (24h SBP), 24 h diastolic blood pressure (24h DBP), daytime systolic blood pressure (dSBP), daytime diastolic blood pressure (dDBP), nighttime systolic blood pressure (nSBP), nighttime diastolic blood pressure (nDBP), 24 h pulse pressure (24h PP), daytime pulse pressure (dPP) and nighttime pulse pressure (nPP).Carotid-radial pulse wave velocity (crPWV) was obtained by Complior device.Results24h SBP, 24h DBP, dSBP, dDBP, nSBP, 24h PP, dPP and nPP were higher in prehypertensive than those in normotensives, but lower in hypertensives (p<0.05 or p<0.01).The crPWV in prehypertensives was 9.67±1.12 m/s, which was higher than that in normotensives (8.27±0.99 m/s), but was lower in hypertensives (10.55±1.71 m/s).Multiple linear regression analysis showed that 24h SBP, 24h PP and nSBP were risk factors for crPWV (β=0.385, 0351 and 0.247；all p<0.05).ConclusionArterial stiffness had been increased in prehypertensives.24h SBP, 24h PP and nSBP are the important influencing factors of arterial stiffness.

Abstract:AimThe present study investigated the effect of lifestyle modification on arterial stiffness in patients with impaired glucose tolerance (IGT).MethodsThere were 162 subjects with IGT were randomized to either a usual care control group (n＝78) or an intensive lifestyle intervention group (n＝84).Both two groups, their height, weight, body mass index and blood pressure were measured.The carotid intima-media thickness (IMT) was measured by ultrasonography, the brachial-ankle pulse wave velocity (baPWV) was measured by automatic atherosclerosis assay instrument at the baseline and after intervention.ResultsThe intervention group showed a significant difference in triglycerides, oral glucose tolerance test 2-hour plasma glucose (OGTT2hPG) following the 24 months of intervention compared with baseline (p<0.01), without differences in changes of the values of carotid IMT and baPWV.At 24 months, significant improvements were noted in most anthropometric values in the intervention group than the control group (p<0.05 or p<0.01), and the values of carotid IMT were significantly lower than the control group (p<0.05), without significant differences in baPWV between the two groups.ConclusionThe finding suggests the active intervention may have beneficial effects on arterial stiffness for the patients at-risk for diabetes through improving anthropometric and certain metabolic outcomes.

Abstract:AimTo investigate the risk factors of leukoaraiosis (LA) in hospitalized patients.MethodsBetween January 2010 to June 2010 in our hospital 509 patients diagnosed by brain magnetic resonance imaging (MRI) as leukoaraiosis were the case group, and 509 cases with no-leukoaraiosis were the control group during the corresponding period.The risk factors of the two groups were inquired according to case-control study design.A database was used and statistical analysis was undergone by SPSS 12.0.ResultsThe unconditional multiple Logistic regression analysis showed that：female ［OR and 95% CI:7.857 (5.139-12.011)］，old age，drink，hypertension in diastolic pressure，cerebral infarction ［OR and 95% CI: 5.591 (3.660-8.541)］，metabolic syndrome were independent risk factors of leukoaraiosis, significantly increasing the risk of leukoaraiosis (all p<0.05).But in these patients, the risk of leukoaraiosis ［OR and 95% CI: 0.699 (0.599-0.815)］ was decreased in patients with increased total cholesterol.ConclusionThe causes of hospitalized patients with leukoaraiosis are various, as a result, early intervention should be aimed at different risk factors to prevent the occurrence of leukoaraiosis.

Abstract:AimTo evaluate the association between carotid arterial stiffness and silent cerebral infarction(SCI) in hemodialysis patients.MethodsTotally 218 hemodialysis patients in our hospital from January 2006 to January 2011 were included in this study.Their clinical and laboratory data were collected.Artherial stiffness index β of common carotid artery was evaluated by an ultrasonic phase-lock Echo-tracking system.Carotid intima media thickness (IMT) was measured by ultrasonography.Carotid plaque score was calculated to quantify the extent of carotid atherosclerosis (CAs) using Sutton’methods.ResultsPearson’s correlation analysis revealed that artherial stiffness parameter β was positively correlated with age, duration of dialysis, mean IMT, and plaque score (p<0.05).Stepwise regression analysis revealed that pulse pressure (β=0.501, p<0.001) and age (β=0.488, p<0.001) were found to be independent determinants of stiffness parameter β, while mean IMT and plaque score as covariates.In addition, stiffness parameter β in patients with SCI was significantly higher than those in patients without SCI (p<0.001), and yet mean IMT and plaque score had no significant difference in both groups.ConclusionThe increased carotid arterial stiffness was associated with the presence of arteriosclerosis and with the presence of SCI in hemodialysis patients.

Abstract:AimTo investigate the change of plasma growth arrest-specific gene 6 (Gas6) level and the relationship of plasma Gas6 level to high sensitivity C-reactive protein (hs-CRP), visfatin, the pulse wave velocity (PWV), and the ankle brachial index (ABI) in the patients with different glucose metabolic status.MethodsPlasma Gas6 and visfatin level were assayed by enzyme-linked immunosorbent assay in type 2 diabetic patients with lower extremity artery diseases (T2DM-LEAD), type 2 diabetic patients (T2DM), patients with impaired glucose regulation (IGR) and normal glucose tolerance (NGT).PWV and ABI were tested by the non-invasive vascular screening device.ResultsPlasma Gas6 level in T2DM-LEAD group was lower than that in T2DM group (p<0.05), and it was significantly reduced in T2DM group compared with NGT group (p<0.01).Pearson’s correlation analysis demonstrated that plasma Gas6 level was negatively correlated with age, duration of diabetes, fasting plasma glucose, 2 h plasma glucose after glucose loading, glycosylated hemoglobin (HbA1c), waist to hip ratio, visfatin, hs-CRP and PWV (p<0.01 or p<0.05), and positively correlated with ABI (p<0.01).Multiple stepwise regression analysis showed waist to hip ratio, HbA1c and hs-CRP were independently related with plasma Gas6 level.ConclusionsThe plasma Gas6 level is lower in patients with IG-R, and is significantly reduced in patients with T2DM and T2DM-LEAD.The change of Gas6 level is associated with inflammation, peripheral vessels atherosclerosis and visceral fat, which may be a potential risk factor of diabetes mellitus.

Abstract:AimTo explore difference of risk factors and coronary artery lesion between Han nationality and Uygur nationality female patients with premature coronary heart disease.Methods490 case of female patients(age≤55 years) clinical data were analyzed retrospectively in our hospital.The coronary artery lesions were evaluated by the dominant type of coronary vessel, location, count, type and Gensini scores.ResultsCompared with the Han group, Uygur group had higher prevalence rate of diabetes, but lower prevalence rate of hypertension (p<0.05).The incidences of left circumflex branch and three-vessel lesion were significantly higher in uygur group than those of Han group（53.8% vs 34.0%,p<0.01;30.3% vs 14.0%, p<0.01）, and the incidences of single-vessel lesion were lower（45.5% vs 67.0%,p<0.01）.The rate of type A was the highest in both Han and Uygur groups.Compared with the Han group, the rate of type A was lower（55.9% vs 72.0%,p<0.001）, and the rate of type B was higher in Uygur group（27.6% vs 16.0%,p<0.05）.Gensini scores of Uyger group was significantly higher than that of Han group(33.8±28.6 vs 21.5±19.5,p<0.01).ConclusionThe main risk factors of female premature coronary heart disease are different between Uygur patients and Han patients, diabetes is the main risk factor in Uygur patients, while hypertension in the Han patients.Severity of coronary artery lesions in Uygur patients is significantly more serious than that in Han patients.

Abstract:AimTo explore the clinical effects of hemoperfusion treatment on hyperlipidemia.Methods We collected 36 cases of hyperlipidemia patients, observed the cilincal indices change of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), fibrinogen and so on before and after hemoperfusion.ResultsAfter hemoperfusion, total cholesterol, triglyceride, low density lipoprotein cholesterol and fibrinogen all decreased significantly, the differences were statistically significant (p<0.05).ConclusionsHemoperfusion cartridge has a strong function to eliminate blood lipid.Hemoperfusion technique is effective in treatment for hyperlipidemia. It’s one of the effective methods to treat hyperlipidemia.

Abstract:AimTo discuss and evaluate the diagnosis, differenis surgical techniques and prognosis of superior mesenteric artery occlusion(SMAO). Methods38 cases of acute superior mesenteric artery occlusion were treated with individualized surgical methods.Acute abdominal pain was the important clinical presentation.All the patients were diagnosed as SMAO by computed tomography arteriography (CTA) before treatment.According to individual condition, 38 patients accepted revascularizations including transcatheterizing thrombolysis, thromboembolectomy, ballon dilatation and stent implant, autologous vein or vascular prosthesis bypass/transplantation and resection of necrotic bowel respectively.ResultsIncision healed by first intention in all patients were followed up 6 months.The circulation of the intestine returned to normal in 30 patients by reexamination of color doppler ultrasonography.4 cases developed short-gut syndrome after operation and 3 cases died, and 2 cases developed intestinal fistula with death of 1 case; Severe infection and multiple organ failure was found in 3 patients and 2 cases died; And there were 6 patients with reperfusion injury, and 6 patients with deterioration of the primary affection and 2 of them died of heart failure.The overall incidence of postoperative complication was 55.2% and the overall mortality rate was 21.1%.ConclusionEarly recognization by appropriate examination and early treatment with individualized surgical methods will improve the prognosis of SMAO.

Abstract:AimTo study the relationship between apolipoprotein C3 gene C3175G polymorphism and overweight-obesity in male, as well as the risk factors of overweight-obesity.MethodsA total 154 normal weight subjects (control group) and 184 overweight-obese subjects(case group) were selected from one hospital in Chongqing during the period of May, 2008 to Sep, 2008.PCR-RFLP was applied to analyze the polymorphism, and logistic regression was used for the analysis of the risk factors of overweight-obesity.ResultsGenotype frequeney distribution of CC,CG and GG were 51.78%, 39.94%, 8.28% respectively, C and G allele frequencies were 71.75% and 28.25%.There was no significant difference of CC genotype, CG genotype, GG genotype and C allele, G allele between case group (47.28%, 44.57%,8.15%, 69.57%, 30.43%) and control group(57.14%, 34.42%, 8.44%, 74.35%,25.65%).In each group, there was no significant difference for the level of age, serum total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) between the 2 C3175G genotypes, except waist-hip ratio (WHR), triglyceride (TG) between the 2 C3175G genotypes in control group.The logistic regression analysis showed that TG may be the risk factor of overweight-obesity.ConclusionThe polymorphism of Apolipoprotein C3 gene C6175G may not be associated with overweight-obesity, but the persons with CC gene type are more likely to suffer from central obesity.

Abstract:AimTo investigate the prevalence of metabolic syndrome(MS) among Xingjiang Han population aged 30-80 and compare the differences of three diagnostic criteria used for MS, which are the National Cholesterol Education Program(ATPⅢ),International Diabetes Federation(IDF) and Chinese Medical Association Diabetes Branch(CDS).MethodsA cross-sectional study was conducted on 2656 Xingjiang Han people aged 30-80.The prevalence of MS and the degree of agreement were both calculated according to the three definitions and the results of MS components were also analyzed.ResultsAccording to the three definitions(ATPⅢ,IDF and CDS),The prevalence(adjusted prevalence) of MS were 27.03%(27.18%),22.40%(20.39%) and 16.04%(16.02%).According to the three definitions(ATPⅢ,IDF and CDS),the prevalence of subjects presenteding at least 3 risk factors were 98.64%,97.9% and 92.81%.Among all the MS subjects, the MS diagnostic criteria of ATPⅢand IDF were in good accordance with Kappa index(0.7801),Youden index(0.7928) and concordance rate(0.9026).CDS was in relatively weak agreement when comparing with other definitions (ATPⅢ and IDF).ConclusionOur findings revealed big differences in the prevalence, the degree of agreement and aggregation of risk components on MS among Xingjiang Han population.IDF criteria’s detection rate of MS was the most, ATPⅢ criteria’s detection rate of subjects presenteding at least 3 risk factors was the most,the concordance of ATPⅢ criteria and IDF was better.

Abstract:It has long been recognized that depression is a risk factor for the development of type 2 diabetes which increased the risk of type 2 diabetes.Recent results suggest that not only depression but also other emotional stress plays an important role in the development of type 2 diabetes.The present review provides an overview of research status of associations between different forms of emotional stress and the development of type 2 diabetes mellitus.

Abstract:20-hydroxyeicosatetraenoic acid（20-HETE) is one of the products of cytochrome P-450 (CYP) catalyzing the ω-hydroxyl of arachidonic acid (AA).With the development of research,it has been discovered that 20-HETE played an important role in the process of organism blood pressure regulation,cerebral blood flow,myocardial contractility and renal function.Furthermore,20-HETE has a close relationship with the occurrence and development of diseases such as tumour,inflammatory function and so on.This article is to summarize the effects of 20-HETE in organism blood pressure and vascular function.